“…Three potential immune modulators in the transcript database, sigma class glutathione transferase (Sigma class GST), cytoplasmic heat shock protein 90 (HSP90), and alpha-enolase (α-Enolase), were selected for further bioinformatic analysis. Protein sequences of recognised Sigma class GST, HSP90 family (alpha and beta isoforms) and α-Enolase from 13, 22, 14 different species, respectively, covering mammalians, nematodes, trematodes, and cestodes were retrieved from Genbank and NCBI Reference Sequence (see recognised Sigma class GST, HSP90 and α-Enolase proteins sequences in Supplementary Table S5 , [ 52 , 56 , 64 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 , 140 , 141 , 142 ]. Recognised sequences were blasted against the A. perfoliata transcriptome (cutoff set at 1.0 × 10 −15 , 1.0 × 10 −20 , and 1.0 × 10 −15 for Sigma GST, HSP90, and α-Enolase, respectively) and representative A. perfoliata sequences translated into protein sequences, as previously described.…”