Identification of novel genes responsible for salt tolerance by transposon mutagenesis in <i>Saccharomyces cerevisiae</i>

Park, Won-Kun; Yang, Ji‐Won; Kim, Hyunsoo

doi:10.1007/s10295-015-1584-y

Cited by 7 publications

(4 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also note that neither haploids or diploids were put through a sexual cycle; allowing for sexual reproduction speeds the rate of adaptation ( McDonald, Rice & Desai, 2016 ). We also find greater fitness increase in complete medium (CM) plus NaCl stress than in CM alone, which was not surprising given what is known about adaptation to NaCl stress in S. cerevisiae ( Blomberg, 1995 ; Dhar et al, 2011 ; Kun, Yang & Kim, 2015 ; Tekarslan-Sahin, Alkim & Sezgin, 2018 ). In contrast, we were surprised to detect less fitness increase in CM plus EtOH stress than in CM alone.…”

Section: Discussionsupporting

confidence: 63%

High-throughput analysis of adaptation using barcoded strains ofSaccharomyces cerevisiae

Fasanello

Liu

Botero

et al. 2020

PeerJ

View full text Add to dashboard Cite

Background Experimental evolution of microbes can be used to empirically address a wide range of questions about evolution and is increasingly employed to study complex phenomena ranging from genetic evolution to evolutionary rescue. Regardless of experimental aims, fitness assays are a central component of this type of research, and low-throughput often limits the scope and complexity of experimental evolution studies. We created an experimental evolution system in Saccharomyces cerevisiae that utilizes genetic barcoding to overcome this challenge. Results We first confirm that barcode insertions do not alter fitness and that barcode sequencing can be used to efficiently detect fitness differences via pooled competition-based fitness assays. Next, we examine the effects of ploidy, chemical stress, and population bottleneck size on the evolutionary dynamics and fitness gains (adaptation) in a total of 76 experimentally evolving, asexual populations by conducting 1,216 fitness assays and analyzing 532 longitudinal-evolutionary samples collected from the evolving populations. In our analysis of these data we describe the strengths of this experimental evolution system and explore sources of error in our measurements of fitness and evolutionary dynamics. Conclusions Our experimental treatments generated distinct fitness effects and evolutionary dynamics, respectively quantified via multiplexed fitness assays and barcode lineage tracking. These findings demonstrate the utility of this new resource for designing and improving high-throughput studies of experimental evolution. The approach described here provides a framework for future studies employing experimental designs that require high-throughput multiplexed fitness measurements.

show abstract

Section: Discussionsupporting

confidence: 63%

High-throughput analysis of adaptation using barcoded strains ofSaccharomyces cerevisiae

Fasanello

Liu

Botero

et al. 2020

PeerJ

View full text Add to dashboard Cite

show abstract

“…Subsequently, 26S rRNA D1/D2 region of each isolate was amplified by polymerase chain reaction (PCR) (Eppendorf, Hamburg, Germany) using NL-1 and NL-4 as forward and reverse primers, respectively. The PCR was performed in a final volume of 25 µL containing 1.5 µL of extracted DNA, 10 µmol/ L of each primer, 0.5 µL of Taq DNA polymerase (2.5 U/µL), 2.5 µL of 10×Taq buffer, 2.0 µL of Mg (13). PCR products (2 µL) were further analyzed on 10 g/L agarose gel containing 0.7 µg/L of EB.…”

Section: T Mmentioning

confidence: 99%

Identification of Zygosaccharomyces mellis strains in stored honey and their stress tolerance

Liu

Tao

Zhu

et al. 2016

Food Sci Biotechnol

View full text Add to dashboard Cite

To screen yeast with high sugar tolerance and evaluate their stress tolerance, six yeast strains were selected from 17 stored honey samples. The species were identified through 26S rRNA sequencing. Their stress tolerance was determined via the Durham fermentation method and ethanol production ability was determined via flask fermentation. The results demonstrated that all the six strains were . Their sugar, ethanol, and acid tolerance ranges were 500-700 g/L, 10-12% (v/v), and pH 2.5-4.5, respectively. The SO tolerance was 250 mg/L. Among the six strains, 6-7431 had the best stress tolerance with sugar tolerance of 700 g/L, ethanol tolerance of 12% (v/v), and acid tolerance of pH 2.5. Furthermore, the strain of 6-7431 had the highest percentage of ethanol production at the same initial sugar content as the other strains. Therefore, the selected six yeast strains would be promising fermentation yeasts for wine-making, ethanol production, or other fermentation purposes.

show abstract

“…However, the regulatory mechanism of how salt-tolerant probiotics survive under salt stress and produce functional metabolites is still unclear. Typical salt-intolerant probiotics, including Lactobacillus, Saccharomyces cerevisiae , and Bifidobacterium , cannot grow when the salt concentration is increased by more than 8% NaCl ( Palomino et al, 2010 ; Park et al, 2015 ; Piqué et al, 2019 ). However, salt-tolerant probiotics have shown great potential for development.…”

Section: Introductionmentioning

confidence: 99%

Salt stress perception and metabolic regulation network analysis of a marine probiotic Meyerozyma guilliermondii GXDK6

et al. 2023

View full text Add to dashboard Cite

IntroductionExtremely salt-tolerant microorganisms play an important role in the development of functional metabolites or drug molecules.MethodsIn this work, the salt stress perception and metabolic regulation network of a marine probiotic Meyerozyma guilliermondii GXDK6 were investigated using integrative omics technology.ResultsResults indicated that GXDK6 could accept the salt stress signals from signal transduction proteins (e.g., phosphorelay intermediate protein YPD1), thereby contributing to regulating the differential expression of its relevant genes (e.g., CTT1, SOD) and proteins (e.g., catalase, superoxide dismutase) in response to salt stress, and increasing the salt-tolerant viability of GXDK6. Omics data also suggested that the transcription (e.g., SMD2), translation (e.g., MRPL1), and protein synthesis and processing (e.g., inner membrane protein OXA1) of upregulated RNAs may contribute to increasing the salt-tolerant survivability of GXDK6 by improving protein transport activity (e.g., Small nuclear ribonucleoprotein Sm D2), anti-apoptotic ability (e.g., 54S ribosomal protein L1), and antioxidant activity (e.g., superoxide dismutase). Moreover, up to 65.9% of the differentially expressed genes/proteins could stimulate GXDK6 to biosynthesize many salt tolerant-related metabolites (e.g., β-alanine, D-mannose) and drug molecules (e.g., deoxyspergualin, calcitriol), and were involved in the metabolic regulation of GXDK6 under high NaCl stress.DiscussionThis study provided new insights into the exploration of novel functional products and/or drugs from extremely salt-tolerant microorganisms.

show abstract

Identification of novel genes responsible for salt tolerance by transposon mutagenesis in Saccharomyces cerevisiae

Cited by 7 publications

References 44 publications

High-throughput analysis of adaptation using barcoded strains ofSaccharomyces cerevisiae

High-throughput analysis of adaptation using barcoded strains ofSaccharomyces cerevisiae

Identification of Zygosaccharomyces mellis strains in stored honey and their stress tolerance

Salt stress perception and metabolic regulation network analysis of a marine probiotic Meyerozyma guilliermondii GXDK6

Contact Info

Product

Resources

About