Identification of novel genes expressed during mouse tooth development by microarray gene expression analysis

Abstract

To identify genes heretofore undiscovered as critical players in the biogenesis of teeth, we have used microarray gene expression analysis of the developing mouse molar tooth (DMT) between postnatal day (P) 1 and P10 to identify genes differentially expressed when compared with 16 control tissues. Of the top 100 genes exhibiting increased expression in the DMT, 29 were found to have been previously associated with tooth development. Differential expression of the remaining 71 genes not previously associated with tooth development was confirmed by quantitative reverse transcription-polymerase chain reaction analysis. Further analysis of seven of the latter genes by mRNA in situ hybridization found that five were specific to the developing tooth in the craniofacial region (Rspo4, Papln, Amtn, Gja1, Maf). Of the remaining two, one was found to be more widely expressed (Sp7) and the other was found to be specific to the nasal serous gland, which is close to, but distinct from, the developing tooth (Vrm). Developmental Dynamics 236: 2245-2257, 2007.

Contrasting Evolutionary Dynamics of the Developmental Regulator PAX9, among Bats, with Evidence for a Novel Post-Transcriptional Regulatory Mechanism

“…However, the possibility of this regulation is contingent upon whether co-expression of Pax9 and Musashi in fact occurs. Important to the findings of the current study, it has been found that Msi-2 was among the top 100 most highly expressed novel gene discoveries in the developing mouse molar [51]. Additional survey of that microarray data set confirmed the co-expression of Msi-2 and Pax9.…”

SLC13A5is the second gene associated with Kohlschütter–Tönz syndrome

“…SLC13A5 expression has been detected from embryonic day 14 in mouse cap stage tooth prior to ameloblasts differentiation and amelogenesis 41. In two independent gene expression profile studies, SLC13A5 showed increased expression during amelogenesis; in particular, SLC13A5 expression was increased in enamel matrix secreting ameloblasts 42 43. Therefore, hypoplastic AI in SLC13A5 -associated KTZS is probably a result of the disturbance of citrate homeostasis, which impairs enamel mineralisation and deprives ameloblasts of citrate necessary for energy metabolism.…”

Chapter 8. Biodiversity and Biogeography of the Moss-mice of New Guinea: A Taxonomic Revision of Pseudohydromys (Muridae: Murinae)

“…Rodent models (especially Mus musculus) are the primary empirical avenue for studies of mammalian developmental dental biology and genetics (cf. Keranen et al, 1998;Line, 2003;Kangas et al, 2004;Ohazama and Sharpe, 2004;Plikus et al, 2005;Osmundsen et al, 2007;Pemberton et al, 2007;Fleischmannova et al, 2008;Caton and Tucker, 2009), and particular genes are known to simultaneously influence molar number, size, shape, and occlusal morphology in Mus. Genomic and developmental studies of hydromyins, particularly Pseudohydromys species if such research ever proves feasible, should offer key insights into the genetic control of molar development.…”