Identification of Novel Candidate Biomarkers for Oral Squamous Cell Carcinoma Based on Whole Gene Expression Profiling

Kengkarn, Sudaporn; Petmitr, Songsak; Boonyuen, Usa; Reamtong, Onrapak; Poomsawat, Sopee; Sanguansin, Sirima

doi:10.1007/s12253-020-00828-w

Cited by 14 publications

(12 citation statements)

References 31 publications

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“…These observations imply that CK17 has a key function in OSCC. The high positive rate of CK17 in OSCC found in our study (90.4%) as well as several studies (79–100%) strongly supports that CK17 plays a pivotal role in OSCC [ 3 , 5 , 6 , 9 , 24 ]. Additionally, CK17 may be an essential protein in OSCC.…”

Section: Discussionsupporting

confidence: 91%

“…Using cDNA microarray analyses of 31 CK genes, CK17 was strongly upregulated in OSCC compared to normal mucosal samples [ 4 ]. Additionally, our previous work has shown that gene expression of CK17 was in the top 20 upregulated genes among 2,724 genes [ 5 ]. The mRNA of CK17 was increased by 25 fold compared with their paired normal oral tissues.…”

Section: Introductionmentioning

confidence: 99%

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Diagnostic Value of Cytokeratin 17 during Oral Carcinogenesis: An Immunohistochemical Study

Sanguansin

Kosanwat

Juengsomjit

et al. 2021

International Journal of Dentistry

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Background. Little is known about the role of cytokeratin 17 (CK17) during oral carcinogenesis. CK17 expression in oral leukoplakia (OL), the most encountered oral potentially malignant disorders and oral squamous cell carcinoma (OSCC), remains very limited. To determine the role of CK17 during oral carcinogenesis and its potential diagnostic marker in oral premalignant and malignant lesions, this study evaluated CK17 expression in OL without dysplasia, OL with dysplasia, and OSCC. CK17 expression in these tissues was compared with those of normal oral mucosa (NOM). Additionally, the relationship between CK17 expression and clinicopathologic factors of OSCC was investigated. Methods. CK17 expression was evaluated in 186 samples consisting of 12 NOM, 33 OL without dysplasia, 58 OL with dysplasia, and 83 OSCC using immunohistochemistry. The proportion of positively immunostained cells was evaluated and scored. Results. CK17 was expressed in 8.3%, 54.5%, 74.1%, and 90.4% of NOM, OL without dysplasia, OL with dysplasia, and OSCC, respectively. NOM had a significantly lower CK17 score than OL with dysplasia ( p = 0.0003 ) and OSCC ( p < 0.0001 ). A significant association between CK17 expression and histopathologic differentiation of OSCC was found. Tumors with well differentiation had high CK17 expression compared with those of moderate and poor differentiation. Conclusion. CK17 was overexpressed in OL with dysplasia and OSCC, suggesting that CK17 plays a pivotal role in the development of premalignant lesions and OSCC. Of clinical significance, CK17 may be a good diagnostic marker for oral premalignant lesions and OSCC. Additionally, CK17 could be used as an objective tool to classify histopathologic grade in OSCC. The findings that CK17 expression is high in OSCC but low in NOM imply that CK17 may serve as a potential therapeutic target for OSCC.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Diagnostic Value of Cytokeratin 17 during Oral Carcinogenesis: An Immunohistochemical Study

Sanguansin

Kosanwat

Juengsomjit

et al. 2021

International Journal of Dentistry

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“…KRT17, one of the keratin proteins overexpressed in our data, activates Akt/mTOR pathway in OSCC cells and favors cell migration and invasion (Khanom et al, 2016). Higher expression of these proteins has been reported in OSCC with the potential of being used for diagnostic purposes (Kengkarn et al, 2020).…”

Section: Discussionmentioning

confidence: 57%

Proteomic Alterations Associated with Oral Cancer Patients with Tobacco Using Habits

Bhat

Mohan

Patil

et al. 2021

OMICS: A Journal of Integrative Biology

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Tobacco abuse is a major risk factor associated with the development of oral squamous cell carcinoma. Differences in molecular aberrations induced by tobacco exposure by chewing or smoking form are not well studied in case of oral cancer. We used tandem mass tag-based quantitative proteomic approach to delineate proteomic alterations in oral cancer patients based on their history of tobacco using habits (patients who chewed tobacco, patients who smoked tobacco, and those with no history of tobacco consumption). Our data identified distinct dysregulation of biological processes and pathways in each patient cohort. Bioinformatics analysis of dysregulated proteins identified in our proteomic study revealed dysregulation of collagen formation and antigen processing/presentation pathway in oral cancer patients who smoked tobacco, whereas proteins associated with the process of keratinization showed enrichment in patients who chewed tobacco. In addition, we identified overexpression of proteins involved in immune pathways and downregulation of muscle contractionmediated signaling events in all three cohorts, irrespective of tobacco using habits. This study lays the groundwork for identification of protein markers that may aid in identification of high-risk patients for cancer development based on the history of tobacco exposure habits.

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“…It was reported that accumulation of S100A7 in the nucleus of cells from HNSC indicated poorer clinical outcomes and thereby served as a biomarker for prognostic prediction. 52 Another published paper demonstrated that S100A7 played an oncogenic role in HNSC through the activation of RAB2A and p38/MAPK pathway. 53 Very recently, it was suggested that S100A7 could act as a good hallmark for the early detection of oral squamous cell carcinoma.…”

Section: Discussionmentioning

confidence: 99%

Diverse Chromobox Family Members: Potential Prognostic Biomarkers and Therapeutic Targets in Head and Neck Squamous Cell Carcinoma

Hu¹,

Yao²,

Zhou³

et al. 2022

IJGM

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Background The Chromobox (CBX) family members were involved in a variety of physiological and oncological processes through the regulation of the epigenetic modification of chromatin. However, the comprehensive analysis of the CBX family in head and neck squamous cell carcinoma (HNSC) is lacking. Methods In this work, we used multiple online databases and tools to investigate the roles of CBX family in aspects of gene expression, prognostic evaluation, genetic alteration, immune micro-environment of tumor, and status of methylation. Results The mRNA expression levels of CBX1, CBX3, and CBX5 were aberrantly increased in patients with HNSC, while CBX7 was aberrantly decreased. Higher expression of CBX7 was significantly associated with longer OS. Within the 5–11% of genetic alteration rate of CBXs, CBX3 ranked the highest and CBX5/7 ranked the lowest. SPRR1B, S100A7, CASP14, CDSN, LCE3D were the top 5 neighbor genes with the strongest association with CBXs in HNSC patients. Signaling pathways such as epidermal cell differentiation, cornification, and peptide cross-linking were demonstrated to have a strong association with CBX genes. The profiles of immune cell infiltration had high similarity for the group of HNSC patients stratified by expression of CBXs. The methylation levels of CBX1 and CBX5 significantly decreased, while that of CBX7 significantly increased in HNSC samples when compared with normal tissue. Conclusion In conclusion, the CBX family showed its valuation for further investigation in HNSC. Our research highlighted that CBX7 had the potential to be a novel diagnostic and prognostic biomarker for patients with HNSC.

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Identification of Novel Candidate Biomarkers for Oral Squamous Cell Carcinoma Based on Whole Gene Expression Profiling

Cited by 14 publications

References 31 publications

Diagnostic Value of Cytokeratin 17 during Oral Carcinogenesis: An Immunohistochemical Study

Diagnostic Value of Cytokeratin 17 during Oral Carcinogenesis: An Immunohistochemical Study

Proteomic Alterations Associated with Oral Cancer Patients with Tobacco Using Habits

Diverse Chromobox Family Members: Potential Prognostic Biomarkers and Therapeutic Targets in Head and Neck Squamous Cell Carcinoma

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