Identification of Novel Bovine Cumulus Cell Molecular Markers Predictive of Oocyte Competence: Functional and Diagnostic Implications1

Bettegowda, Anilkumar; Patel, Osman V.; Lee, Kyung Bon; Park, Ki Eun; Salem, Mohamed; Yao, Jianbo; Ireland, James J.; Smith, George W.

doi:10.1095/biolreprod.107.067223

Cited by 123 publications

(113 citation statements)

References 65 publications

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“…Although a potential effect of the enzymatic method in cumulus cell gene expression profile cannot be ruled out, we thought that mechanical isolation of cumulus cells before ICSI could jeopardise the success of our patients' fertility treatment rendering our strategy unethical. Nevertheless, hyaluronidase has been used by other researchers in cumulus cell gene expression studies even in non-human mammals (cows) [2,3].…”

Section: Discussionmentioning

confidence: 99%

LH receptor gene expression in cumulus cells in women entering an ART program

Papamentzelopoulou

Mavrogianni

Partsinevelos

et al. 2012

J Assist Reprod Genet

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Purpose Luteinizing hormone (LH) exerts its actions through its receptor (LHR), which is mainly expressed in theca cells and to a lesser extent in oocytes, granulosa and cumulus cells. The aim of the present study was the investigation of a possible correlation between LHR gene and LHR splice variants expression in cumulus cells and ovarian response as well as ART outcome. Methods Forty patients undergoing ICSI treatment for male factor infertility underwent a long luteal GnRH-agonist downregulation protocol with a fixed 5-day rLH pretreatment prior to rFSH stimulation and samples of cumulus cells were collected on the day of egg collection. RNA extraction and cDNA preparation was followed by LHR gene expression investigation through real-time PCR. Furthermore, cumulus cells were investigated for the detection of LHR splice variants using reverse transcription PCR. Results Concerning LHR expression in cumulus cells, a statistically significant negative association was observed with the duration of ovarian stimulation (odds ratio00.23, p00.012). Interestingly, 6 over 7 women who fell pregnant expressed at least two specific types of LHR splice variants (735 bp, 621 bp), while only 1 out of 19 women that did not express any splice variant achieved a pregnancy. Conclusions Consequently, the present study provide a step towards a new role of LHR gene expression profiling as a biomarker in the prediction of ovarian response at least in terms of duration of stimulation and also a tentative role of LHR splice variants expression in the prediction of pregnancy success.

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Section: Discussionmentioning

confidence: 99%

LH receptor gene expression in cumulus cells in women entering an ART program

Papamentzelopoulou

Mavrogianni

Partsinevelos

et al. 2012

J Assist Reprod Genet

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“…Several cathepsin transcripts, including CTSB and CTSZ, are inversely related to oocyte quality. In one study, cumulus derived from COCs of prepubertal heifers, a model of poor oocyte competency, contained more CTSB mRNA than COCs from mature cows (Bettegowda et al 2008). Oocyte quality and postfertilization development could be improved by exposing COCs to a membrane-permeable cathepsin inhibitor (Bettegowda et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Fibroblast growth factor 10 enhances bovine oocyte maturation and developmental competence in vitro

Zhang¹,

Hansen²,

Ealy³

2010

Reproduction

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The ability of oocytes to resume meiosis, become fertilized, and generate viable pregnancies is controlled during folliculogenesis by several endocrine and paracrine factors. The aim of this work is to determine whether fibroblast growth factor 10 (FGF10) is an oocyte competent factor. Transcripts for each of the four FGF receptor types (FGFR) were present in cumulus and oocytes after their extraction from the follicles. FGFR1 transcripts predominated in cumulus cells whereas FGFR2 was most abundant in oocytes. Exposing the cumulus-oocyte complexes to FGF10 during in vitro maturation did not affect cleavage rates, but increases (P!0.05) in the percentage of embryos at the 8-16-cell stage on day 3 and at the blastocyst stage on day 7, which were evident in FGF10-supplemented oocytes. The progression of oocytes through meiosis and cumulus expansion was increased (P!0.05) by FGF10. The importance of the endogenous sources of FGFs was examined by adding anti-FGF10 IgG during oocyte maturation. Blocking endogenous FGF10 activity decreased (P!0.05) the percentage of oocytes developing into blastocysts and limited (P!0.05) cumulus expansion. Expression profiles of putative cumulus and oocyte competency markers were examined for their involvement in FGF10-mediated responses. FGF10 influenced the expression of CTSB and SPRY2 in cumulus cells and BMP15 in oocytes. In summary, this work provides new insight into the importance of FGFRs and locally derived FGF10 during oocyte maturation in cattle. Its subsequent impact on in vitro embryo development implicates it as a noteworthy oocyte competent factor.

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“…The quality of oocytes and the fate of embryos are determined by the maternal legacy (Bettegowda et al, 2008;Lechniak et al, 2008). It has been considered that some of the specific molecules, which are not well known, determine oocyte quality.…”

Section: Introductionmentioning

confidence: 99%

“…With that, FST could have a potential and functional role in embryonic development that affects embryo quality (Patel et al, 2007;Bettegowda et al, 2008). Therefore, it was hypothesized that FST supplementation would enhance the embryonic development when it was treated in culture medium during in vitro culture of porcine embryos after IVF.…”

Section: Introductionmentioning

confidence: 99%

Follistatins have potential functional role in Porcine Embryogenesis

Kim¹,

Chun²,

Lee³

et al. 2016

Korean Journal of Agricultural Science

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In animal reproduction, the quality of oocytes and embryos has been evaluated by the expression of specific molecules. Follistatin (FST), which was isolated from follicular fluid, binds and bio-neutralizes the TGF-β superfamily members. Previous studies using the bovine model showed FST could be an important molecular determinant of embryo developmental competence. However, the effect of FST treatment on porcine embryo developmental competence has not been established. In this study, the effect of exogenous FST on porcine embryo developmental competence was investigated during in vitro culture. FST (10 ng/ml) treatment induced a significant decrease in the rate of cell arrest at the 4-cell stage. The expression levels of DNA-methyltransferase 1 (DNMT1), histone deacetylase 1 (HDAC1), and histone deacetylase 2 (HDAC2) were decreased in 4-cell stage embryos. FST treatment also resulted in significant improvements in developmental competence of embryos in terms of blastocyst formation rate and OCT-4 mRNA levels, the latter being related to pluripotency. In conclusion, during in vitro culture, FST treatment significantly ameliorated 4-cell block during embryonic development and improved embryo developmental competence. Therefore, FST treatment may potentially have a functional role in porcine embryogenesis that is broadly applicable to enhance in vitro embryo development.

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Identification of Novel Bovine Cumulus Cell Molecular Markers Predictive of Oocyte Competence: Functional and Diagnostic Implications1

Cited by 123 publications

References 65 publications

LH receptor gene expression in cumulus cells in women entering an ART program

LH receptor gene expression in cumulus cells in women entering an ART program

Fibroblast growth factor 10 enhances bovine oocyte maturation and developmental competence in vitro

Follistatins have potential functional role in Porcine Embryogenesis

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