Identification of novel bioactive proteins and their produced oligopeptides from Torreya grandis nuts using proteomic based prediction

Durrani, Rabia; Yang, Meiyun; Yang, Baoru; Durand, Erwann; Delavault, André; Hou, Baorong; Huan, Weiwei; Yiyang, Long; Lili, Song; Gao, Fei

doi:10.1016/j.foodchem.2022.134843

Cited by 5 publications

(4 citation statements)

References 29 publications

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“…The major SDS-PAGE bands and characteristic HPLC peaks showed that protein fractions of higher intensities were consistent in toad venom, likely because of the structural stability of some peptides in toad venom [37]. The differences in the color depth of the bands and the size of the chromatographic peak areas indicated that the HZ and LYG specimens showed the greatest differences in protein quality.…”

Section: Discussionmentioning

confidence: 97%

Identification of Protein Quality Markers in Toad Venom from Bufo gargarizans

et al. 2023

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Toad venom is a traditional Chinese medicine with high medicinal value. The existing quality evaluation standards of toad venom have obvious limitations because of the lack of research on proteins. Thus, it is necessary to screen suitable quality markers and establish appropriate quality evaluation methods for toad venom proteins to guarantee their safety and efficacy in clinical applications. SDS-PAGE, HPLC, and cytotoxicity assays were used to analyze differences in protein components of toad venom from different areas. Functional proteins were screened as potential quality markers by proteomic and bioinformatic analyses. The protein components and small molecular components of toad venom were not correlated in content. Additionally, the protein component had strong cytotoxicity. Proteomics analysis showed that 13 antimicrobial proteins, four anti-inflammatory and analgesic proteins, and 20 antitumor proteins were differentially expressed extracellular proteins. A candidate list of functional proteins was coded as potential quality markers. Moreover, Lysozyme C-1, which has antimicrobial activity, and Neuropeptide B (NPB), which has anti-inflammatory and analgesic activity, were identified as potential quality markers for toad venom proteins. Quality markers can be used as the basis of quality studies of toad venom proteins and help to construct and improve safe, scientific, and comprehensive quality evaluation methods.

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Section: Discussionmentioning

confidence: 97%

Identification of Protein Quality Markers in Toad Venom from Bufo gargarizans

et al. 2023

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“…[15][16][17][18][19][20] However, the structure and function of antibacterial amino acid polymers were limited by the class of available amino acids with positive charge, such as lysine or arginine. [21][22][23][24] Herein, we designed the sulfonium cationic poly(methionine)s as a class of synthetic AMP synthetic mimics because of many advantages of poly(methionine)s, such as excellent biocompatibility, low-cost and nontoxicity (Fig. 1).…”

mentioning

confidence: 99%

Combating drug-resistant bacteria with sulfonium cationic poly(methionine)

Zhu,

Li,

Huan

2023

RSC Adv.

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“…Protein separation was performed using HPLC according to the method described by Gu et al, with some modifications [ 24 ]. The extracted saffron protein was filtered using a 0.22 µm filter membrane and then analyzed by HPLC.…”

Section: Methodsmentioning

confidence: 99%

Identification and Functional Validation of Two Novel Antioxidant Peptides in Saffron

Long,

Tao,

Wang

et al. 2024

Antioxidants

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Saffron (Crocus sativus L.) is one of the most expensive spices in the world, boasting rich medicinal and edible value. However, the effective development of active natural substances in saffron is still limited. Currently, there is a lack of comprehensive studies on the saffron stigma protein, and the main effect peptides have not been identified. In this study, the total protein composition of saffron stigmas was analyzed, and two main antioxidant peptides (DGGSDYLGK and VDPYFNK) were identified, which showed high antioxidant activity. Then, the stability of two peptides was further evaluated. Furthermore, our results suggested that these two peptides may protect HepG2 cells from H2O2-induced oxidative damage by significantly improving the activity of endogenous antioxidant enzymes and reducing the malondialdehyde (MDA) content. Collectively, we identified two peptides screened from the saffron protein possessing good antioxidant activity and stability, making them promising candidates for use as functional foods, etc., for health promotion. Our findings indicated that proteomic analysis together with peptide identification is a good method for exploitation and utilization of spice plants.

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Identification of novel bioactive proteins and their produced oligopeptides from Torreya grandis nuts using proteomic based prediction

Cited by 5 publications

References 29 publications

Identification of Protein Quality Markers in Toad Venom from Bufo gargarizans

Identification of Protein Quality Markers in Toad Venom from Bufo gargarizans

Combating drug-resistant bacteria with sulfonium cationic poly(methionine)

Identification and Functional Validation of Two Novel Antioxidant Peptides in Saffron

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