Identification of novel B-cell epitopes on the capsid protein of type 1 porcine astrovirus, using monoclonal antibodies

Replication of Porcine Astrovirus Type 1-Infected PK-15 Cells In Vitro Affected by RIG-I and MDA5 Signaling Pathways

“…To fix the PK-15 cells, cold acetone was applied for 30 min at −20°C, followed by five washes with PBS. PBS containing 0.5% Triton X-100 was used to permeabilize the cells for 20 min, and 5% BSA was then used to block the cells for 1 h. PAstV1 Cap-specific antibodies ( 49 ) were then added, and the cells were transferred to 4°C for 16 h. After five more washes with PBS, anti-mouse IgG(H+L)–Alexa Fluor 488 (Abmart, Inc., Shanghai, China) was added, and the cells were incubated for 1 h at 25°C. 4′,6-Diamidino-2-phenylindole (DAPI; Servicebio, China) was used for nuclear staining.…”