Identification of Novel and Differentially Expressed MicroRNAs in Goat Enzootic Nasal Adenocarcinoma

Wang, Bin; Ni, Ye; Cao, Sanjie; Wen, Xintian; Huang, Yongxs; Yan, Qigui

doi:10.1101/057885

Cited by 2 publications

(2 citation statements)

References 52 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5), and we conclude that JSRV does not, in fact, encode a miRNA. This is consistent with small RNA-Seq studies of other betaretroviruses, including MMTV (31) and enzootic nasal tumor virus (83) and a bioinformatics screen of all retroviral genome sequences (30).…”

Section: Discussionsupporting

confidence: 88%

Jaagsiekte sheep retrovirus infection induces changes in microRNA expression in the ovine lung

Garcia

Karagianni

Vasoya

et al. 2021

Preprint

View full text Add to dashboard Cite

Ovine pulmonary adenocarcinoma (OPA) is an infectious neoplastic lung disease of sheep caused by jaagsiekte sheep retrovirus. OPA is an important veterinary problem and is also a valuable large animal model for human lung adenocarcinoma. JSRV infects type 2 alveolar epithelial cells in the lung and induces the growth of tumors, but little is known about the molecular events that lead to the activation of oncogenic pathways in infected cells. MicroRNAs (miRNAs) are small RNA molecules of approximately 22 nucleotides with important roles in regulating gene expression in eukaryotes and with well-established roles in cancer. Here we used small-RNA sequencing to investigate the changes in miRNA expression that occur in JSRV-infected ovine lung. After filtering out low abundance miRNAs, we identified expression of 405 miRNAs, 32 of which were differentially expressed in JSRV-infected lung compared to mock-inoculated control lung. Highly upregulated miRNAs included miR-182, miR-183, miR-96 and miR-135b, which have also been associated with oncogenic changes in human lung cancer. Network analysis of genes potentially targeted by the deregulated miRNAs identified their involvement in pathways known to be dysregulated in OPA. We found no evidence to support the existence of miRNAs encoded by JSRV. This study provides the first information on miRNA expression in OPA and identifies a number of targets for future studies into the role of these molecules in the pathogenesis of this unique veterinary model for human lung adenocarcinoma.IMPORTANCEOvine pulmonary adenocarcinoma is a neoplastic lung disease of sheep caused by jaagsiekte sheep retrovirus (JSRV). OPA is a significant welfare and economic concern for sheep producers and is a valuable large animal model for human lung adenocarcinoma. MicroRNAs are small RNA molecules of approximately 22 nucleotides with important functions in regulating gene expression in eukaryotes and with well-established roles in cancer. In this study, we examined the changes in microRNA expression that occur in the lung in response to JSRV infection. We identified differential expression of a number of host-encoded microRNAs in infected tissue, including microRNAs with roles in human cancer. We found no evidence that JSRV encodes a microRNA. This study provides new insights on the cellular response to JSRV infection in the ovine lung, which will inform future studies into the pathogenesis of OPA in sheep and its use as a model for human lung adenocarcinoma.

show abstract

Section: Discussionsupporting

confidence: 88%

Jaagsiekte sheep retrovirus infection induces changes in microRNA expression in the ovine lung

Garcia

Karagianni

Vasoya

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Six CVS and six SVS tissue samples were cut into pieces and dipped in RNAstore Reagent (TIANGEN, Beijing, China) immediately after resection and shipped on dry ice to Jing Neng Bio-Technology corporation (Shanghai, China) for high-throughput sequencing as described previously [37]. A miRNA library of VS tissue was created by combining the sequencing data from all twelve samples.…”

Section: Mirna Sequencingmentioning

confidence: 99%

chr19_34670, a newly identified miRNA, represses rapid tumor growth of cystic vestibular schwannomas by targeting transforming growth factor α and inhibiting MAPK pathway

Huo¹,

Yan²,

Wang³

et al. 2018

Oncotarget

View full text Add to dashboard Cite

Purpose: Cystic vestibular schwannoma (CVS) is an aggressive form of vestibular schwannoma (VS) with rapid growth and poor outcomes. The molecular basis of CVS remains unclear. In this study, we aimed to investigate whether microRNAs (miRNAs) contribute to the development of CVS. Methods: We identified miRNAs that were differentially expressed between CVS and solid VS (SVS) tissues using high-throughput sequencing and quantitative real-time PCR analysis. Gene targets of the identified miRNA were predicted by bioinformatics tools and validated by luciferase reporter analysis. The effects of the miRNA and its target genes on the proliferation of primary VS cells were examined using CCK-8, flow cytometry, colony formation assay, and western-blot. Results: We identified a novel miRNA, chr19_34670, downregulated in CVS compared to SVS. chr19_34670 directly targeted the 3' untranslated region of transforming growth factor α (TGFα) mRNA and suppressed its expression. TGFα overexpression motivated VS cell proliferation, while this effect could be attenuated by chr19_34670. We also found that chr19_34670 inhibited the function of key proteins in the MAPK pathway by suppressing phosphorylation of ERK and MEK. Conclusions: chr19_34670 suppresses the aggressive proliferation observed in CVS by inhibiting TGFα expression and phosphorylated proteins in the MAPK pathway.

show abstract

Identification of Novel and Differentially Expressed MicroRNAs in Goat Enzootic Nasal Adenocarcinoma

Cited by 2 publications

References 52 publications

Jaagsiekte sheep retrovirus infection induces changes in microRNA expression in the ovine lung

Jaagsiekte sheep retrovirus infection induces changes in microRNA expression in the ovine lung

chr19_34670, a newly identified miRNA, represses rapid tumor growth of cystic vestibular schwannomas by targeting transforming growth factor α and inhibiting MAPK pathway

Contact Info

Product

Resources

About