Identification of novel and conserved microRNAs involved in fruit development and ripening in Fragaria vesca

Abstract

MicroRNAs (miRNAs) are class of noncoding RNAs that regulate gene expression at the post-transcriptional level, either by endonucleolytic cleavage or by translational inhibition. Strawberry is a popular worldwide fresh fruit and is believed to benefit human health. However, the function of miRNAs during this fruit development and ripening remains unknown and miRNAs for specific for these processes are expected to be discovered. In the study, we identified 218 conserved miRNAs and 87 novel miRNAs in <em>Fragaria vesca</em>. Expression profiling of miRNAs during fruit development and ripening was performed, and the expression of targets of the miRNAs was validated by qRT-PCR (quantitative reverse transcription polymerase chain reaction). This study provided data for further research on molecular mechanisms involved in fruit development and ripening.

Identification of Known and Novel MicroRNAs in Raspberry Organs Through High-Throughput Sequencing

“…Compared with the annotated miRNAs of miRBase, we found that the raspberry miRNAs were relatively conserved with those of higher model and closely relative plants, such as Arabidopsis (Wang et al, 2004), Fragaria vesca (Han et al, 2019), Zea mays (Aydinoglu and Lucas, 2019), and sweet cherry (Wang et al, 2019). A large number miRNAs, such as miR159, miR157 and miR394 have similar base composition with miRNAs in closely relative plants.…”

“…In recent years, miRNAs have been identified in some plants species through cloning and computational approaches (Saliminejad et al, 2019;Smoczynska et al, 2019), and it has also been shown that miRNAs may be predicted using modern machine learning approaches (Parveen et al, 2019;Esposito et al, 2020). Recent reports have identified hundreds of miRNAs in several species, including Fragaria vesca (Han et al, 2019), cardamom (Anjali et al, 2019), sweet cherry (Wang et al, 2019), and Brazilian pine (Galdino et al, 2019) through highthroughput sequencing. Currently, 38,589 entries representing hairpin precursor miRNAs and 48,860 mature miRNAs have been discovered from 271 organisms, and these are available in the public miRNA database miRbase (Release 22.1, October 2018) 1 (Kozomara et al, 2018).…”