Identification of new up-regulated genes under drought stress in soybean nodules

The Legume Root Nodule: From Symbiotic Nitrogen Fixation to Senescence

“…The accumulation of catalytic Fe has been detected during dark stress (Gogorcena et al, 1997;Becana & Klucas, 1992) and drought stress (Gogorcena et al, 1995) and participates in OH  production during dark stress (Becana & Klucas, 1992). The modification of iron metabolism during SIS is also noticeable through the up regulation of ferritin and metallothionein during drought stress (Clement et al, 2008) and dark stress (Perez Guerra et al, 2010). These proteins sequester free Fe to decrease the Fenton reaction and protect the cellular primary components.…”

Plant N Fluxes and Modulation by Nitrogen, Heat and Water Stresses: A Review Based on Comparison of Legumes and Non Legume Plants

“…Moreover, it has been shown that under severe drought conditions, the nodule leghemoglobin content was negatively affected (Manavalan et al, 2009;Naya et al, 2007), impacting once more on the O 2 availability to the bacteroids (Marino et al, 2006). In parallel, a gene coding for a ferritin is largely up-regulated under water stress conditions, suggesting an important iron trafficking in stressed nodules, being the consequence of the leghemoglobin degradation (Clement et al, 2008). Another mechanism related to the inhibition of N 2 fixation involves a N feedback, mediated by various molecules depending on the plant species such as asparagine (Bacanamwo & Harper, 1997), glutamine (Neo & Layzell, 1997), proline, histidine, tryptophane (Larrainzar et al, 2009), ureides (Serraj et al, 2001 or even a combination between high rates of nodule asparagine and ureide and a transport of amino acids from the leaves (King & Purcell, 2005).…”

Molecular Responses to Osmotic Stresses in Soybean

“…Furthermore, the homologue of a member of CaM-binding protein family was identified from a suppression subtractive hybridization (SSH) cDNA library from nodular tissue of soybean under drought stress (Clement et al, 2008). Interestingly, nodules development and maintenance are affected by osmotic stresses via a Ca 2+ mediated pathway (Guenther et al, 2003) Under salt and drought stresses, a CDPK residing on the soybean symbiosome membrane can phosphorylate nodulin 26, a water channel on the same membrane, to control its water permeability (Guenther et al, 2003).…”

“…GmSTY1 can be induced by salt and drought treatments but not exogenous ABA, indicating that this protein kinase may respond to abiotic stresses in an ABA-independent pathway. From a soybean SSH library (Clement et al, 2008), a putative Ste-20 related kinase was identified. In yeast, Ste-20 is a kinase essential for osmotic stress signalling via the SHO1 branch of the high osmolarity glycerol (HOG) MAPK pathway, as shown by complementation tests using yeast mutants (Raitt et al, 2000).…”