Identification of new sources for brown planthopper resistance from rice germplasm and introgression lines derived from <i>O. nivara</i>

Akanksha, I; Lakshmi, V. Jhansi; Chirutkar, PM; Rao, LV Subba; Sarla, N.; Ram, T.

doi:10.5958/2249-5266.2017.00037.6

Cited by 2 publications

(1 citation statement)

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“…Bph17 was reported at map distances of 3.2 cM from RM5953, while the mapped QTL in this study is 3.5 cM from RM5953. Furthermore, resistance screening showed a differential response to resistance, as RPBio4918-230S was found to be resistant at the maximum tillering stage while Rathu Heenati did not show tolerance at this stage [29]. It has also been reported that Rathu Heenati is resistant to all four BPH biotypes at the seedling stage but susceptible at the tillering stage [30].…”

Section: Discussionmentioning

confidence: 98%

Identification and Functional Characterization of Two Major Loci Associated with Resistance against Brown Planthoppers (Nilaparvata lugens (Stål)) Derived from Oryza nivara

Srivastava,

Pusuluri,

Balakrishnan

et al. 2023

Genes

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The brown planthopper (BPH) is a highly destructive pest of rice, causing significant economic losses in various regions of South and Southeast Asia. Researchers have made promising strides in developing resistance against BPH in rice. Introgression line RPBio4918-230S, derived from Oryza nivara, has shown consistent resistance to BPH at both the seedling and adult stages of rice plants. Segregation analysis has revealed that this resistance is governed by two recessive loci, known as bph39(t) and bph40(t), contributing to 21% and 22% of the phenotypic variance, respectively. We later mapped the genes using a backcross population derived from a cross between Swarna and RPBio4918-230S. We identified specific marker loci, namely RM8213, RM5953, and R4M17, on chromosome 4, flanking the bph39(t) and bph40(t) loci. Furthermore, quantitative expression analysis of candidate genes situated between the RM8213 and R4M17 markers was conducted. It was observed that eight genes exhibited up-regulation in RPBio4918-230S and down-regulation in Swarna after BPH infestation. One gene of particular interest, a serine/threonine-protein kinase receptor (STPKR), showed significant up-regulation in RPBio4918-230S. In-depth sequencing of the susceptible and resistant alleles of STPKR from Swarna and RPBio4918-230S, respectively, revealed numerous single nucleotide polymorphisms (SNPs) and insertion–deletion (InDel) mutations, both in the coding and regulatory regions of the gene. Notably, six of these mutations resulted in amino acid substitutions in the coding region of STPKR (R5K, I38L, S120N, T319A, T320S, and F348S) when compared to Swarna and the reference sequence of Nipponbare. Further validation of these mutations in a set of highly resistant and susceptible backcross inbred lines confirmed the candidacy of the STPKR gene with respect to BPH resistance controlled by bph39(t) and bph40(t). Functional markers specific for STPKR have been developed and validated and can be used for accelerated transfer of the resistant locus to elite rice cultivars.

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Section: Discussionmentioning

confidence: 98%