Identification of new S-RNase self-incompatibility alleles and characterization of natural mutations in Iranian almond cultivars

Hafizi, Akram; Shiran, Behrouz; Maleki, Bahram; Imani, Ali; Banović, Bojana

doi:10.1007/s00468-012-0803-7

Cited by 11 publications

(12 citation statements)

References 61 publications

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“…A table of almond cross-incompatibility was constructed using the results of the present work and the plentiful data reported by other authors (Ortega et al, 2006;López et al, 2006;Barckley et al, 2006;Kodad et al, 2008;Valizadeh et al, 2009;Kodad and Socias i Company, 2009;Halász et al, 2010;Kodad et al, 2010;Fernández i Martí et al, 2010;Martínez-García et al, 2011;Mousavi et al, 2011;Hafizi et al, 2013;Curró et al, 2015). This new table was not intended to be merely an update of the earlier ones, but a compilation or review table in which all the self-incompatible almond cultivars S-genotyped to date were to be included, and thus it was made after an accurate correction of the inconsistencies detected in previous works.…”

Section: Revision and Updating Of Almond Cross-incompatibility Groupsmentioning

confidence: 84%

“…Other primers have also been used to assess the Sgenotype in almond (Channuntapipat et al, 2003;López et al, 2006;Barckley et al, 2006;Halász et al, 2008;Kodad et al, 2008;Kodad et al, 2010;Curró et al, 2015). The information obtained with the different methods and primer sets indicated has been included in different versions of a table of almond cross-incompatibility groups (Kester et al, 1994;Bošković et al, 2003;Barckley et al, 2006;Ortega et al, 2006;Kodadand Socias i Company, 2009;Hafizi et al, 2013). However, to date no attempt has been made to reconcile the information of all these tables neither to correct possible inconsistencies.…”

Section: Introductionmentioning

confidence: 99%

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Cross-incompatibility in the cultivated almond (Prunus dulcis): Updating, revision and correction

Gómez

Dicenta

Batlle

et al. 2019

Scientia Horticulturae

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Most almond cultivars are self-incompatible, and so to obtain a yield they need to be pollinated with cross-compatible cultivars. For an efficient use of these cultivars in growing and breeding, over the years many have been S-genotyped. This information has been included in consecutive tables of crossincompatibility groups, which are essentially an update of the previously proposed. However, the information of these tables has not always been reconciled and their inconsistencies have not been corrected. In this work the S-genotypes of 15 Spanish almond local cultivars, included in a research program for the preservation of almond biodiversity, were determined for the first time. For this, PCR with consensus primers for the Prunus S-RNases, PCR using S-RNase allele specific primers designed herein, and also cloning and sequencing were performed. As a result, a new S-RNase allele numbered as S53 could be identified, and a compilation table of almond cross-incompatibility groups is provided including the information from this and previous studies. In this novel Table 12 new crossincompatibility groups have been established, two former groups were omitted, and those cultivars with inconsistent genotypes have been removed. The information of this table will facilitate the use of a very high number of almond cultivars in research and breeding. Determination of the S-genotype might be considered as an important addition to a set of identity markers of almond genetic resources, what is of particular interest in the characterization of the agricultural biodiversity.

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Section: Revision and Updating Of Almond Cross-incompatibility Groupsmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Cross-incompatibility in the cultivated almond (Prunus dulcis): Updating, revision and correction

Gómez

Dicenta

Batlle

et al. 2019

Scientia Horticulturae

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“…One exact way to determine S-genotype of pear cultivars is by identification of self-incompatibility alleles using allele-specific PCR amplification. This method is already used for identification of S-genotypes in almond (Ortega et al 2006;Valizadeh and Ershadi 2009;Hafizi et al 2013;Mousavi et al 2014), apple Janssens et al 1995;Ershadi et al 2006), apricot (Vilanova et al 2006;Murathan et al 2017), sweet cherry (Szikriszt et al 2013), Japanese pear (Ishimizu et al 1999;Takasaki et al 2004;Kim et al 2007) and European pear (Moriya et al 2007;Mota et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Self-Incompatibility Alleles in Iranian Pear Cultivars

Bagheri

Ahmad

2019

Preprint

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“…In Prunus, including almond, SI is gametophytic and under the genetic control of complex and highly variable S loci. Based on the results of experimental crosses, there are thought to be at least 50 variants at the almond S-locus [1][2][3][4][5] . Sequencing of a 71,953 bp region of one haplotype (S 7 , also known as S c ) showed that the almond S locus includes S-locus F-box (SLF), stylar RNase (S-RNase) and S-haplotype-specific F-box (SFB) genes, other open reading frames and pairs of long-terminal-repeat retrotransposons (LTRs) 6 .…”

mentioning

confidence: 99%

“…For this complex locus, variant forms of individual genes are referred to as alleles, while variant forms of the entire locus are referred to as haplotypes. Although the S 7 haplotype is the only one for which a nearly complete sequence has been published, the SLF, S-RNase and/or SFB alleles of some other haplotypes have been fully or partially sequenced [1][2][3][4][5] . Among haplotypes that have been physically mapped, the order and orientations of S-locus features are conserved, but the distances between these features vary 6 .…”

mentioning

confidence: 99%

Variation among S-locus haplotypes and among stylar RNases in almond

Goonetilleke

Croxford

March

et al. 2020

Sci Rep

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in many plant species, self-incompatibility systems limit self-pollination and mating among relatives. this helps maintain genetic diversity in natural populations but imposes constraints in agriculture and plant breeding. in almond [Prunus dulcis (Mill.) D.A. Webb], the specificity of self-incompatibility is mainly determined by stylar ribonuclease (S-Rnase) and S-haplotype-specific F-box (SFB) proteins, both encoded within a complex locus, S. prior to this research, a nearly complete sequence was available for one S-locus haplotype. Here, we report complete sequences for four haplotypes and partial sequences for 11 haplotypes. Haplotypes vary in sequences of genes (particularly S-RNase and SFB), distances between genes and numbers and positions of long terminal repeat transposons. Haplotype variation outside of the S-RNase and SFB genes may help maintain functionally important associations between S-RNase and SFB alleles. fluorescence-based assays were developed to distinguish among some S-RNase alleles. With three-dimensional modelling of five S-RNase proteins, conserved active sites were identified and variation was observed in electrostatic potential and in the numbers, characteristics and positions of secondary structural elements, loop anchoring points and glycosylation sites. A hypervariable region on the protein surface and differences in the number, location and types of glycosylation sites may contribute to determining S-RNase specificity.

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Identification of new S-RNase self-incompatibility alleles and characterization of natural mutations in Iranian almond cultivars

Cited by 11 publications

References 61 publications

Cross-incompatibility in the cultivated almond (Prunus dulcis): Updating, revision and correction

Cross-incompatibility in the cultivated almond (Prunus dulcis): Updating, revision and correction

Self-Incompatibility Alleles in Iranian Pear Cultivars

Variation among S-locus haplotypes and among stylar RNases in almond

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