Identification of new genes of nodule bacteria Sinorhizobium meliloti involved in the control of efficiency of symbiosis with alfalfa Medicago sativa

Онищук, О. П.; Chizhevskaya, E. P.; Курчак, О. Н.; Andronov, E. E.; Симаров, Б. В.

doi:10.1134/s2079059715020070

Cited by 7 publications

(6 citation statements)

References 14 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PCR amplification was performed according to standard protocol (Weisburg et al, 1991). The obtained PCR fragments were isolated from agarose gel (Onishchuk et al, 2015) and sequenced using the ABI PRISM 3500xl (Applied Biosystems, Waltham, MA, USA). The sequences were compared with the sequence of the 16S rRNA gene of the strain Ch-13 B. subtilis, available in the GenBank database (Accession Number: MW050985).…”

Section: Identification Of Bacterial Strainsmentioning

confidence: 99%

Nitrogen use by plants and nitrogen flows after application of standard and biomodified nitrogen fertilizers on barley

Завалин

Chebotar

Алферов

et al. 2021

BioComm

View full text Add to dashboard Cite

The aim of our study was to assess the efficiency of application of biomodified nitrogen fertilizers for barley, to reveal the sources of nitrogen used for biomass formation with the use of the 15N stable isotope, and to study nitrogen flows in the system of fertilizers–soil–plants–atmosphere. We demonstrated in a model experiment the ability of the plant growth-promoting bacteria Bacillus subtilis Ch-13 to move from the granules of mineral fertilizers to plant roots and to colonize them effectively. The effectiveness of biomodified nitrogen fertilizers for barley, Nur variety, was assessed in a microfield trial. After the application of biomodified nitrogen fertilizers, the accumulation of 15N in the plants increased by 2–5 %, its incorporation in the soil decreased and gaseous losses were decreased by 7 % as compared with the use of the usual forms of fertilizers. The application of biomodified nitrogen fertilizers can be used in agricultural practice as a novel technology to regulate nitrogen flows in the system of fertilizers–soil–plants–atmosphere.

show abstract

Section: Identification Of Bacterial Strainsmentioning

confidence: 99%

Nitrogen use by plants and nitrogen flows after application of standard and biomodified nitrogen fertilizers on barley

Завалин

Chebotar

Алферов

et al. 2021

BioComm

View full text Add to dashboard Cite

show abstract

“…PCR amplifications were performed using Pfu polymerase (Thermo Fisher Scientific, Waltham, MA, USA) according to standard protocol: initial denaturation at 95°C for 3 min, followed by 30 cycles at denaturation at 94°C for 30 s, primer annealing at 52°C for 30 s, extension at 72°C for 1 min, and a final extension lasting 4 min. PCR fragments were extracted from agarose gel (Onishchuk et al, 2015), with each PsMT-gene ligated with a nifH promoter. The obtained ligase mixtures were then used as a matrix in the PCR with primers nifH-F and MT1-R (for fusion with PsMT1) or with primers nifH-F and MT2-R (for fusion with PsMT2).…”

Section: Bacterial Strain Constructionmentioning

confidence: 99%

Efficacy of a Plant-Microbe System: Pisum sativum (L.) Cadmium-Tolerant Mutant and Rhizobium leguminosarum Strains, Expressing Pea Metallothionein Genes PsMT1 and PsMT2, for Cadmium Phytoremediation

et al. 2020

View full text Add to dashboard Cite

Two transgenic strains of Rhizobium leguminosarum bv. viciae, 3841-PsMT1 and 3841-PsMT2, were obtained. These strains contain the genetic constructions nifH-PsMT1 and nifH-PsMT2 coding for two pea (Pisum sativum L.) metallothionein genes, PsMT1 and PsMT2, fused with the promoter region of the nifH gene. The ability of both transgenic strains to form nodules on roots of the pea wild-type SGE and the mutant SGECd t , which is characterized by increased tolerance to and accumulation of cadmium (Cd) in plants, was analyzed. Without Cd treatment, the wild type and mutant SGECd t inoculated with R. leguminosarum strains 3841, 3841-PsMT1, or 3841-PsMT2 were similar histologically and in their ultrastructural organization of nodules. Nodules of wild-type SGE inoculated with strain 3841 and exposed to 0.5 μM CdCl 2 were characterized by an enlarged senescence zone. It was in stark contrast to Cd-treated nodules of the mutant SGECd t that maintained their proper organization. Cadmium treatment of either wild-type SGE or mutant SGECd t did not cause significant alterations in histological organization of nodules formed by strains 3841-PsMT1 and 3841-PsMT2. Although some abnormalities were observed at the ultrastructural level, they were less pronounced in the nodules of strain 3841-PsMT1 than in those formed by 3841-PsMT2. Both transgenic strains also differed in their effects on pea plant growth and the Cd and nutrient contents in shoots. In our opinion, combination of Cd-tolerant mutant SGECd t and the strains 3841-PsMT1 or 3841-PsMT2 may be used as an original model for study of Cd tolerance mechanisms in legume-rhizobial symbiosis and possibilities for its application in phytoremediation or phytostabilization technologies.

show abstract

“… Kang et al (2019) revealed the potential of acdS gene to improve the growth of tomatoes and further tolerate salinity stress as a result of the regulation of secondary metabolite which goes with our study for being abundant in HR. mtaD gene induces plant growth, improves fruit ripening, and produce stimulates the production of the enzyme that belongs to the family of metallodependent hydrolases and involves phosphonates metabolism, together with various pesticides and herbicides ( Onishchuk et al, 2015 ). This gene is known for preventing the poisoning of crop plants caused by chemical pesticides and herbicides applied to farmland ( Loeschcke & Thies, 2015 ; Onishchuk et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Unraveling the functional genes present in rhizosphere microbiomes of Solanum lycopersicum

Adedayo¹,

Fadiji²,

Babalola³

2023

PeerJ

View full text Add to dashboard Cite

The microbiomes living in the rhizosphere soil of the tomato plant contribute immensely to the state of health of the tomato plant alongside improving sustainable agriculture. With the aid of shotgun metagenomics sequencing, we characterized the putative functional genes (plant-growth-promoting and disease-resistant genes) produced by the microbial communities dwelling in the rhizosphere soil of healthy and powdery mildew-diseased tomato plants. The results identified twenty-one (21) plant growth promotion (PGP) genes in the microbiomes inhabiting the healthy rhizosphere (HR) which are more predomiant as compared to diseased rhizosphere (DR) that has nine (9) genes and four (4) genes in bulk soil (BR). Likewise, we identified some disease-resistant genes which include nucleotide binding genes and antimicrobial genes. Our study revealed fifteen (15) genes in HR which made it greater in comparison to DR that has three (3) genes and three (3) genes in bulk soil. Further studies should be conducted by isolating these microorganisms and introduce them to field experiments for cultivation of tomatoes.

show abstract

Identification of new genes of nodule bacteria Sinorhizobium meliloti involved in the control of efficiency of symbiosis with alfalfa Medicago sativa

Cited by 7 publications

References 14 publications

Nitrogen use by plants and nitrogen flows after application of standard and biomodified nitrogen fertilizers on barley

Nitrogen use by plants and nitrogen flows after application of standard and biomodified nitrogen fertilizers on barley

Efficacy of a Plant-Microbe System: Pisum sativum (L.) Cadmium-Tolerant Mutant and Rhizobium leguminosarum Strains, Expressing Pea Metallothionein Genes PsMT1 and PsMT2, for Cadmium Phytoremediation

Unraveling the functional genes present in rhizosphere microbiomes of Solanum lycopersicum

Contact Info

Product

Resources

About