Identification of New Diterpenoids from Euphorbia calyptrata Cell Cultures

Abstract

Four new diterpenoids, helioscopinolides F (2), H (4), I (5), and L (6), were isolated from three different cultured cell lines of Euphorbia calyptrata var. involucrata. Helioscopinolide B (1) and jolkinolide E (3), previously found in Euphorbia helioscopia and Euphorbia jolkini, respectively, were also isolated from the same cultures. Structures were identified by spectroscopic methods.

Regulated Expressions of MMP-2, -9 by Diterpenoids from Euphorbia formosana Hayata

“…The aerial parts of E. formosana were extracted initially using methanol, and further partitioned between CHCl 3 and H 2 O. The CHCl 3 layer was then subjected to gravity silica column separation followed by HPLC purification to give two new diterpenoids 1 and 2 , along with helioscopinolide A ( 3 ) [ 9 ], helioscopinolide B ( 4 ) [ 10 ], helioscopinolide C ( 5 ) [ 11 ] and ent -(5β,8α,9β,10α,12α)-12-hydroxyatis-16-ene-3,14-dione ( 6 ) [ 12 ].…”

13C-NMR Data of Three Important Diterpenes Isolated from Euphorbia Species

“…Affected by the α,β-unsaturated γ-lactone, the carbon chemical shifts of Me-17 is very low at δ C 6.7–10.0, as shown in 1 – 7 , 12 , 14 – 18 , 21 – 23 , 25 and 30 – 34 . Thus, the assignment of the four methyl groups (C-17, C-18, C-19 and C-20) in compounds 19 – 20 and 26 – 29 [ 40 , 42 ] were doubtful. In their 13 C-NMR spectra, chemical shifts around δ C 8.0 should be assigned to C-17 instead of C-20, δ C 27.0-34.0 should be assigned to C-18 instead of C-17, and δ C 16.0–23.0 should be assigned to C-19 instead of C-18.…”

Bioactive 18(4 → 3)-abeo-abietanoid derivatives from the leaves of Tripterygium wilfordii

“…[9][10][11][12] Anti-inammatory, immunosuppressive and anti-cancer activities of those diterpene lactone epoxide compounds have been extensively reported. [14][15][16][17][18][19] In consideration of the chemical investigations of T. wilfordii, several abietanes and 18(4 / 3)-abeo-abietanes were obtained. As a result, six new abietane derivative tripterlides A-F (1-6), together with six known 18(4 / 3)-abeoabietanoids were isolated from the ethanolic extract of the leaves of T. wilfordii by several chromatographic technologies ( Fig.…”