Identification of new biomarkers of human endometrial receptivity in the natural cycle

Haouzi, D.; Mahmoud, Kamel Z.; Fourar, M.; Bendhaou, K.; Déchaud, H.; Vos, John De; Rème, Thierry; Dewailly, Didier; Hamamah, S.

doi:10.1093/humrep/den360

Cited by 163 publications

(124 citation statements)

References 35 publications

Supporting

Mentioning

121

Contrasting

Unclassified

Order By: Relevance

“…EG-VEGF may support pregnancy through angiogenesis and VP regulation [5,25]. It has been reported that EG-VEGF is expressed in human periimplantation endometrium, which expression and receptor are elevated in human decidua during early pregnancy, and that EG-VEGF can mediate fetal-maternal dialogue by regulating hCG-mediated endometrial LIF expression [26,27].…”

Section: Discussionmentioning

confidence: 99%

Effects of EG-VEGF, VEGF and TGF-β1 on pregnancy outcome in patients undergoing IVF-ET treatment

Gao

Zhao

Lin

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To investigate the correlation of endocrine glandderived vascular endothelial growth factor (EG-VEGF), vascular endothelial growth factor (VEGF) and transforming growth factor beta 1 (TGF-β1) with the corresponding reproductive outcome in patients who received in vitro fertilizationembryo transfer (IVF-ET). Methods Sixty-seven women undergoing IVF-ET at a university tertiary hospital were recruited for a prospective study. Concentrations of EG-VEGF, VEGF and TGF-β1 were measured by enzyme-linked immunosorbent assay (ELISA) in follicular fluid (FF) collected during oocyte retrieval (OR) and in serum collected 2 days after OR. Results In FF, concentrations of both EG-VEGF and VEGF were negatively correlated with peak E2 and the number of MII oocytes retrieved, and positively correlated with each other. In serum, concentrations of all the three growth factors were positively correlated with the rate of good quality embryo, and with one another. Patients in the pregnancy group had lower peak E2 concentrations and higher serum EG-VEGF concentrations than those in the non-pregnancy group, but such tendency was not observed in the case of VEGF and TGF-β1. Conclusions Both concentrations of EG-VEGF and VEGF in FF were negatively correlated with ovarian response and oocyte maturation. Concentrations of all the three growth factors in serum were positively correlated with embryo quality, but only serum concentrations of EG-VEGF were associated with the pregnancy outcome.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of EG-VEGF, VEGF and TGF-β1 on pregnancy outcome in patients undergoing IVF-ET treatment

Gao

Zhao

Lin

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…The gene expression profile temporally associated with the acquisition of endometrial receptivity has been established in untreated normal women utilizing microarrays (Carson et al 2002, Kao et al 2002, Borthwick et al 2003, Riesewijk et al 2003, Mirkin et al 2005, Haouzi et al 2009, Talbi et al 2006, but the effect of LNG on global gene expression profile in the human endometrium has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Effect of single post-ovulatory administration of levonorgestrel on gene expression profile during the receptive period of the human endometrium

Vargas¹,

Tapia-Pizarro²,

Henríquez³

et al. 2011

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

The hypothesis that levonorgestrel (LNG) used as an emergency contraceptive interferes with endometrial receptivity remains unproven. We compared the endometrial gene expression profile during the receptive period after administering a single dose of LNG 1 . 5 mg or placebo on day 1 of the luteal phase. An endometrial biopsy was done on day LHC7 or LHC8 and samples were taken from seven volunteers, each one contributing with one cycle treated with placebo and another with LNG. The expression of 20 383 genes was determined using cDNA microarrays. Real-time RT-PCR was used 1) to confirm the differences found in DNA microarray analysis and 2) to determine the effect of LNG on transcript levels of C3, C4BPa, COX2, MAOA, S100A4, and SERPINB9, known to be upregulated during receptivity, and on cPLA2a, JAK1, JNK1, CTSL1, and GSTP1, known to respond to mifepristone. Additional endometrial biopsies were done during the pre-receptive (LHC3) and receptive (LHC7) period and samples were taken from eight untreated volunteers in order to determine the changes associated with acquisition of receptivity of 14 genes. Mean levels of PAEP, TGM2, CLU, IGF2, and IL6ST mRNAs increased after administering LNG while those of HGD, SAT1, EVA1, LOC90133, ANXA1, SLC25A29, CYB5A, CRIP1, and SLC39A14 decreased. Except for the level of ANXA1 transcript, all changes remained within the range observed in untreated controls, and none of the transcripts responding to mifepristone changed in response to LNG. Post-ovulatory administration of LNG caused minimal changes in gene expression profiling during the receptive period. Neither the magnitude nor the nature or direction of the changes endorses the hypothesis that LNG interferes with endometrial receptivity.

show abstract

“…Recent studies performed by Haouzi and associates have found new genes expressed during the implantation window by the human endometrium. [Haouzi et al, 2009] This information along with knowledge of previously discussed biomarkers can lead investigators to a more thorough approach when performing endometrial biopsies during a natural cycle especially in patients who have had unsuccessful IVF cycles. The goal of such investigation is to better understand the requirements of a hospitable environment for blastocyst implantation.…”

Section: Discussionmentioning

confidence: 99%

Biomarkers Related to Endometrial Receptivity and Implantation

Trolice¹,

Amyradakis²

2012

Advances in Embryo Transfer

View full text Add to dashboard Cite

Identification of new biomarkers of human endometrial receptivity in the natural cycle

Cited by 163 publications

References 35 publications

Effects of EG-VEGF, VEGF and TGF-β1 on pregnancy outcome in patients undergoing IVF-ET treatment

Effects of EG-VEGF, VEGF and TGF-β1 on pregnancy outcome in patients undergoing IVF-ET treatment

Effect of single post-ovulatory administration of levonorgestrel on gene expression profile during the receptive period of the human endometrium

Biomarkers Related to Endometrial Receptivity and Implantation

Contact Info

Product

Resources

About