Identification of neutrophil‐related genes and development of a prognostic model for cholangiocarcinoma

Li, Jianfeng; Xiong, Ji; Wang, Lin; Zhang, Mengyang; Yi, Jing; Liu, Longzi

doi:10.1002/jgm.3569

Cited by 1 publication

(1 citation statement)

References 54 publications

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“…Our findings on the paradoxical activation of EBF3 in melanoma, influenced by methylation changes, intersect with observations by Li et al on the IFN pathway in cholangiocarcinoma [ 61 ]. This comparison suggests a potentially broader role for the IFN pathway in cancer, beyond its known immune functions.…”

Section: Discussionsupporting

confidence: 92%

Targeted DNA Methylation Editing Using an All-in-One System Establishes Paradoxical Activation of EBF3

Banerjee,

Ajithkumar,

Keestra

et al. 2024

Cancers

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Cutaneous melanoma is rapidly on the rise globally, surpassing the growth rate of other cancers, with metastasis being the primary cause of death in melanoma patients. Consequently, understanding the mechanisms behind this metastatic process and exploring innovative treatments is of paramount importance. Recent research has shown promise in unravelling the role of epigenetic factors in melanoma progression to metastasis. While DNA hypermethylation at gene promoters typically suppresses gene expression, we have contributed to establishing the newly understood mechanism of paradoxical activation of genes via DNA methylation, where high methylation coincides with increased gene activity. This mechanism challenges the conventional paradigm that promoter methylation solely silences genes, suggesting that, for specific genes, it might actually activate them. Traditionally, altering DNA methylation in vitro has involved using global demethylating agents, which is insufficient for studying the mechanism and testing the direct consequence of gene methylation changes. To investigate promoter hypermethylation and its association with gene activation, we employed a novel approach utilising a CRISPR-SunTag All-in-one system. Here, we focused on editing the DNA methylation of a specific gene promoter segment (EBF3) in melanoma cells using the All-in-one system. Using bisulfite sequencing and qPCR with RNA-Seq, we successfully demonstrated highly effective methylation and demethylation of the EBF3 promoter, with subsequent gene expression changes, to establish and validate the paradoxical role of DNA methylation. Further, our study provides novel insights into the function of the EBF3 gene, which remains largely unknown. Overall, this study challenges the conventional view of methylation as solely a gene-silencing mechanism and demonstrates a potential function of EBF3 in IFN pathway signalling, potentially uncovering new insights into epigenetic drivers of malignancy and metastasis.

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Section: Discussionsupporting

confidence: 92%