Identification of Neuroactive Constituents of the Ethyl Acetate Fraction from Cyperi Rhizoma Using Bioactivity-Guided Fractionation

Present study reports metabolites profiling, antioxidant, and antidiabetic effects of Cuscuta reflexa grown on Casearia tomentosa. The UHPLC-QTOF-MS/MS method was used to characterize phytochemicals in most potent extract. Thirteen bioactive compounds/metabolites including (2S)-3-(β-D-galactopyranosyloxy)-2-(hexanoyloxy)propyl nonanoate, 4-O-β-D-glucosyl-4-coumaric acid, isorhamnetin-3-O-glucoside, 7-(α-D-glucopyranosyloxy)-2,3,4,5,6-pentahydroxyheptanoic acid, 13Shydroxyoctadecadienoic acid, astragaloside derivative, salicylic acid, oleanolic acid, citric acid, pinellic acid, quinic acid, caffeic acid derivative and caffeic acid-3-glucoside were detected in 80% hydroethanolic (HE) extract. Among different extracts (aqueous, 20-80% HE and pure ethanolic), 80% HE extract showed maximum TPC (186.19 ± 2.15 mg GAE/g DE) and TFC (106.50 ± 1.68 mg RE/g DE). A strong correlation was observed between TPC, TFC, DPPH radical scavenging (IC 50 = 60.64 ± 1.74 µg/mL) and TAP activity (235.96 ± 1.33 mg AAE/g DE) of 80% HE extract among others. The same extract also depicted highest α-amylase and α-glucosidase enzyme inhibition with IC 50 of 71.84 ± 1.06 and 57.25 ± 1.40 µg/mL, respectively. Molecular docking was performed to explore possible role of identified phytochemicals. Binding affinity data as well as interaction patterns have revealed possible interaction of the identified bioactives against α-glucosidase and α-amylase. Moreover, intra-molecular charge transfer, energies of the FMOs, HOMO-LUMO-E gaps , MEP and IP were also explained. The present findings suggested that, C. reflexa is a promising natural antioxidant and antidiabetic agent, thus supporting the use of this plant for designing related functional foods and nutra-pharmaceuticals.

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“…[66] This compound is reported to exhibit mild antioxidant effect based on DPPH antiradical assay with IC 50 value >500 μM. [67] Compound (13)…”

Section: Uhplc-qtof-ms/ms Based Metabolite Profile Of C Reflexamentioning

confidence: 99%

UHPLC-QTOF-MS/MS metabolites profiling and antioxidant/antidiabetic attributes of Cuscuta reflexa grown on Casearia tomentosa: exploring phytochemicals role via molecular docking

Nadeem

Mumtaz

Danish

et al. 2020

International Journal of Food Properties

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“…However, to the best of our knowledge, there is no evidence supporting the effect of active constituents of CRE in gynecologic disorders including endometriosis. In a previous study, we found that α-cyperone is the most abundant and active component of CRE [ 44 , 45 , 46 , 47 ]. In these regards, we have examined the anti-endometriotic effect of α-cyperone in human endometriotic 12Z cells.…”

Section: Discussionmentioning

confidence: 99%

The Anti-Endometriotic Effect of Cyperi Rhizoma Extract, Inhibiting Cell Adhesion and the Expression of Pain-Related Factors through Akt and NF-kB Pathways

et al. 2022

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Rhizomes of Cyperus rotundus have been widely used as a traditional medicine in Asia for the treatment of gynecological diseases. However, there is no scientific evidence demonstrating the effect of C. rotundus rhizomes on endometriosis, which is characterized by the adhesion of endometrial tissues outside the uterus, resulting in chronic and severe pelvic pain. The aim of this study was to investigate the effects of Cyperi rhizoma extract (CRE) on cell adhesion and the expression of pain-related factors (neurotrophins) in endometriotic cells, and to elucidate the underlying molecular mechanisms. CRE inhibited the adhesion of human endometriotic 12Z cells to peritoneal mesothelial Met5A cells using by adhesion assays. The mRNA expression of adhesion molecules [P-cadherin and matrix metalloproteinase (MMP)-2] was downregulated by CRE treatment. In addition, CRE significantly inhibited the mRNA expression of neurotrophins (BDNF, NGF, NT-3 and NT-4/5) in 12Z cells. Moreover, Akt overexpression markedly neutralized the inhibition of cell adhesion by CRE and expression of neurotrophins in 12Z cells. Furthermore, it was found that CRE suppressed NF-kB activation through the Akt pathway. These data suggest that CRE exerts anti-endometriotic activities by the inhibition of cell adhesion and neurotrophin expression, through the negative regulation of the Akt and NF-kB pathways in endometriotic cells.

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“…Chuanxiong Rhizoma is known to improve blood circulation, removing blood stasis, promoting qi and relieving pain [2]. Cyperi Rhizoma can regulate qi, relieve depression, regulate menstruation and relieve pain [3]. The use of Chuanxiong Rhizoma-Cyperi Rhizoma combination is first stated in Danxi Xinfa (a comprehensive medical book written by Zhu Zhenheng in 1481) for the alleviation of headache with a syndrome of phlegm syncope and migraine headache [4].…”

Section: Introductionmentioning

confidence: 99%

Effects of Chuanxiong Rhizoma-Cyperi Rhizoma Combination on Migraine in a Murine Model

Niu¹

2021

FARMACIA

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Chuanxiong Rhizoma-Cyperi Rhizoma combination is a traditional anti-migraine treatment used for years in Traditional Chinese Medicine. This study aimed to evaluate the efficacy and the underlying mechanism of using this combination in a murine model of migraine. Forty-eight male Wistar rats were randomly divided into 6 groups, a normal group, a migraine model group, a Flunarizine group used as a positive therapeutic effect group and high, medium, and low dose Chuanxiong Rhizoma-Cyperi Rhizoma combination groups. Seven days before migraine induction, the animals from the Flunarizine group received 0.43 mg/kg bw/day flunarizine, the animals from Chuanxiong Rhizoma-Cyperi Rhizoma combination groups received 6, 3 and 1.5 g/kg bw/day of alcoholic extract powder, while the normal group and the model group received the same amount of saline solution. After the model induction, the behavioural signs of migraine, calcitonin gene-related peptide (CGRP), interleukin-1β (IL-1β), tumour necrosis factor-α (TNF-α) levels both in serum and brain tissue, nitric oxide (NO) levels, nitric oxide synthase (NOS) activity and nitric oxide/endothelin (NO/ET) ratio in serum significantly increased compared with the normal group, while the levels of 5-hydroxytryptamine (5-HT), β-Endorphin (β-EP) both in serum and brain tissue and ET in serum significantly decreased. The pre-treatment with Flunarizine and increasing doses of Chuanxiong Rhizoma-Cyperi Rhizoma combination groups significantly reversed. The effect on increasing the 5-HT levels and decreasing IL-1β, TNF-α n, NOS and NO levels was superior for the high dose group compared with the Flunarizine group. In conclusion, Chuanxiong Rhizoma-Cyperi Rhizoma combination showed beneficial effects in a migraine murine model by modulating the neurogenic inflammation and the levels of neurotransmitter and vasomotor factors. RezumatCombinația Chuanxiong Rhizoma-Cyperi Rhizoma este un tratament anti-migrenos folosit în medicina tradițională chineză. Acest studiu a avut ca scop evaluarea eficacității și a mecanismului ce stă la baza utilizării acestei combinații într-un model murin de migrenă. Patruzeci și opt de șobolani masculi Wistar au fost împărțiți în mod aleatoriu în 6 grupuri: un grup de control, un grup model de migrenă, un grup Flunarizină utilizat ca martor pozitiv și 3 grupuri cărora li s-a administrat combinația Chuanxiong Rhizoma-Cyperi Rhizoma în doză mare, medie și mică. Cu șapte zile înainte de debut, animalele din grupul Flunarizină au primit 0,43 mg/kg corp/zi flunarizină, animalele din grupurile cărora li s-a administrat combinația Chuanxiong Rhizoma-Cyperi Rhizoma au primit 6, 3 și 1,5 g/kg corp/zi de extract alcoolic, în timp ce grupul normal și grupul model au primit aceeași cantitate de soluție salină. După inducerea migrenei au fost evaluate semnele de comportament specifice și nivelurile peptidei asociate genei calcitoninei (CGRP), interleukinei-1β (IL-1β), factorului de necroză tumorală-α (TNF-α), în ser și în țesutul cerebral. Nivelurile oxidului nitric (NO), activita...

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Identification of Neuroactive Constituents of the Ethyl Acetate Fraction from Cyperi Rhizoma Using Bioactivity-Guided Fractionation

Cited by 12 publications

References 45 publications

UHPLC-QTOF-MS/MS metabolites profiling and antioxidant/antidiabetic attributes of Cuscuta reflexa grown on Casearia tomentosa: exploring phytochemicals role via molecular docking

UHPLC-QTOF-MS/MS metabolites profiling and antioxidant/antidiabetic attributes of Cuscuta reflexa grown on Casearia tomentosa: exploring phytochemicals role via molecular docking

The Anti-Endometriotic Effect of Cyperi Rhizoma Extract, Inhibiting Cell Adhesion and the Expression of Pain-Related Factors through Akt and NF-kB Pathways

Effects of Chuanxiong Rhizoma-Cyperi Rhizoma Combination on Migraine in a Murine Model

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