Identification of neural stem and progenitor cell subpopulations using DC insulator-based dielectrophoresis

Liu, Yameng; Jiang, Alan; Kim, Estelle; Ro, Clarissa C.; Adams, Tayloria N.G.; Flanagan, Lisa A.; Taylor, Thomas J.; Hayes, Mark A.

doi:10.1039/c9an00456d

Cited by 27 publications

(31 citation statements)

References 34 publications

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“…First is that the effects of biologically important changes may not induce a measurable change in the cells using the electric field effects. The current study undermines this concept, along with many previous quantitative assessments (Jones et al, 2014(Jones et al, , 2015Crowther et al, 2019;Hilton and Hayes, 2019;Liu et al, 2019). The second theme is that the variation in the biological entities will be too great to decipher.…”

Section: Discussionmentioning

confidence: 70%

“…Dielectrophoresis and cellular impedance spectroscopy have demonstrated a capability to differentiate cells based upon changes in the biochemical makeup of the cellular structure with labels (Labeed et al, 2003(Labeed et al, , 2011Chin et al, 2006;Coley et al, 2007;Flanagan et al, 2008;Jones et al, 2015;Su et al, 2016;Fernandez et al, 2017;Rohani et al, 2018;Crowther et al, 2019;Liu et al, 2019; Hilton et al, 2020). When fully developed and vetted, this approach of serotyping will require less expense and expertise compared to producing antisera in agglutination test utilizing O and H antiserum and will not require expert genomic information interpretation skills in whole genome sequencing for identifying Salmonella (Ng and Kirkness, 2010;Ashton et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Differential Biophysical Behaviors of Closely Related Strains of Salmonella

Liu

Hayes

2020

Front. Microbiol.

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Salmonella is an important pathogen and is a worldwide threat to food safety and public health. Surveillance of serotypes and fundamental biological and biochemical studies are supported by a wide variety of established and emerging bioanalytical techniques. These include classic serotyping based on the Kauffmann-White nomenclature and the emerging whole genome sequencing strategy. Another emerging strategy is native whole cell biophysical characterization which has yet to be applied to Salmonella. However, this technique has been shown to provide high resolution differentiation of serotypes with several other paired strains of other microbes and pathogens. To demonstrate that biophysical characterization might be useful for Salmonella serotyping, the closely related strains sv. Cubana and sv. Poona were chosen for study. These two serovars were subjected to biophysical measurements on a dielectrophoresisbased microfluidic device that generated full differentiation of the unlabeled and native cells. They were differentiated by the ratio of electrophoretic (EP) to dielectrophoretic (DEP) mobilities. This differentiation factor is 2.7 ± 0.3 × 10 10 V/m 2 for sv. Cubana, versus 2.2 ± 0.3 × 10 10 V/m 2 for sv. Poona. This work shows for the first time the differentiation, concentration, and characterization of the Salmonella serotypes by exploiting their biophysical properties. It may lead to a less expensive and more decentralized new tool and method for microbiologists, complimenting and working in parallel with other characterization methods.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Differential Biophysical Behaviors of Closely Related Strains of Salmonella

Liu

Hayes

2020

Front. Microbiol.

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“…Another great example for an area expanded from EK research is the electrophysiological characterization of neural stem and progenitor cells [55]. Liu et al.…”

Section: Analyzing and Sensing Of Mammalian Cellsmentioning

confidence: 99%

Analysis of microorganisms with nonlinear electrokinetic microsystems

Hakim

Lapizco‐Encinas

2021

Electrophoresis

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Nonlinear electrokinetics (EK), specifically electrophoresis of the second kind, dielectrophoresis (DEP) and electrorotation (EROT), have gained significant interest recently for their flexibility and labeless discriminant manner of operation. The current applications of these technologies are a clear advancement from what they were when first discovered, but also still show strong signs of future growth. The present review article presents a discussion of the current uses of microscale nonlinear EK technologies as analytical, sensing, and purification tools for microorganisms. The discussion is focused on some of the latest discoveries with various nonlinear EK microfluidic techniques, such as DEP particle trapping and EROT for particle assessments, for the analysis of microorganisms ranging from viruses to parasites. Along the way, special focus was given to key research articles from within the past two years to provide the most up‐to‐date knowledge on the current state‐of‐the‐art within the field of microscale EK, and from there, an outlook on where the future of the field is headed is also included.

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“…2E). Other designs feature one [68] or several constrictions [69,70] or classify particles due to deflection into different outlets [71,72]. Electrode-based separators usually operate at low voltages (around 10 V) and high frequencies to exploit differences in the first cross-over frequency of cells (Fig.…”

Section: Devices For Dielectrophoretic Separationmentioning

confidence: 99%

A review of dielectrophoretic separation and classification of non‐biological particles

Pesch

2020

Electrophoresis

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A review of dielectrophoretic separation and classification of non-biological particles Dielectrophoresis (DEP) is a selective electrokinetic particle manipulation technology that is applied for almost 100 years and currently finds most applications in biomedical research using microfluidic devices operating at moderate to low throughput. This paper reviews DEP separators capable of high-throughput operation and research addressing separation and analysis of non-biological particle systems. Apart from discussing particle polarization mechanisms, this review summarizes the early applications of DEP for dielectric sorting of minerals and lists contemporary applications in solid/liquid, liquid/liquid, and solid/air separation, for example, DEP filtration or airborne fiber length classification; the review also summarizes developments in DEP fouling suppression, gives a brief overview of electrocoalescence and addresses current problems in high-throughput DEP separation. We aim to provide inspiration for DEP application schemes outside of the biomedical sector, for example, for the recovery of precious metal from scrap or for extraction of metal from low-grade ore.

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Identification of neural stem and progenitor cell subpopulations using DC insulator-based dielectrophoresis

Abstract: This study presents an unbiased high-resolution separation and characterization of NSPC subpopulations using direct current insulator-based dielectrophoresis.

Cited by 27 publications

References 34 publications

Differential Biophysical Behaviors of Closely Related Strains of Salmonella

Differential Biophysical Behaviors of Closely Related Strains of Salmonella

Analysis of microorganisms with nonlinear electrokinetic microsystems

A review of dielectrophoretic separation and classification of non‐biological particles

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