Identification of NDRG1 as an early inducible gene during in vitro maturation of cultured mast cells

Taketomi, Yoshitaka; Sugiki, Toshihiko; Saito, Takanori; Ishii, Shin; Hisada, Masato; Suzuki-Nishimura, Tamiko; Uchida, Masaatsu K.; Moon, Tae Chul; Chang, Hyeun Wook; Natori, Yasuhiro; Miyazawa, Shinobu; Kikuchi-Yanoshita, Rei; Murakami, Makoto; Kudo, Ichiro

doi:10.1016/s0006-291x(03)00942-2

Cited by 48 publications

(71 citation statements)

References 28 publications

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“…Molecules involved in cancer metastasis might serve as markers for early detection of metastasis, prognostic judgment and/or as targets for therapeutic intervention [24,25] . Most studies showed that NDRG1 was involved in cellular growth [4,[26][27][28] , differentiation [29][30][31] , tumorigenesis [32] , metastasis [14] and poor clinical outcome of some tumor [33] . Our study showed that in normal colon NDRG1 protein was expressed mainly on the surface of epithelial cells that border the gut lumen, and the result was in accordance with recent reports [12] , indicating that NDRG1 protein was expressed late during differentiation, just before apoptosis and shedding of cells into the colon lumen.…”

Section: Discussionmentioning

confidence: 99%

Correlation of N-myc downstream-regulated gene 1 overexpression with progressive growth of colorectal neoplasm

Wang¹,

Wang²,

Wang³

et al. 2004

WJG

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AIM:To study the function of N-myc downstream-regulated gene 1 (NDRG1) in colorectal carcinogenesis and its correlation with tumor lymph node metastasis. METHODS:NDRG1 was detected at its protein level by immunohistochemistry (IHC) and image analysis (IA), and NDRG1 mRNA was detected by in situ hybridization (ISH) in formalin-fixed and paraffin-embedded sections with a total of 190 specimens including 38 normal colorectal mucosae, 31 colorectal adenomas, 45 non-metastatic colorectal carcinomas (CRCs), 38 metastatic primary CRC and subsequently regional lymph nodes respectively. At the same time, the correlations of NDRG1 with sex, age of patients and histological types of colorectal carcinomas were observed. RESULTS: NDRG1 proteins were gradually increased in colorectal carcinogenesis (P<0.05 or P<0.01). There was a significant difference in the expression of NDRG1 between non-metastatic and metastatic CRCs (P<0.05), and the correlation was positive (P<0.01, r s =0.329). However, there was no obvious difference in the expression of NDRG1 between the primary sites of CRCs and that in the metastatic sites of corresponding regional lymph nodes, nor was there an apparent difference in sex, age, and histological types. The expression of NDRG1 mRNA was generally in concordance with that of NDRG1 protein.CONCLUSION: NDRG1 gene may play an important role in colorectal carcinogenesis. In addition, NDRG1 may be a putative tumor metastasis promoter gene and is regarded as one of the molecular biological markers that can forecast early metastasis of CRCs. NDRG1 gene in the metastatic sites of regional lymph nodes may preserve its expression characteristics in the primary sites of CRCs to some extent. The expression of NDRG1 is not affected by sex, age and histological types. The role of NDRG1 in tumor metastatic process can be demonstrated by in vivo and in vitro.

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Section: Discussionmentioning

confidence: 99%

Correlation of N-myc downstream-regulated gene 1 overexpression with progressive growth of colorectal neoplasm

Wang¹,

Wang²,

Wang³

et al. 2004

WJG

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“…2F). Even after coculture with fibroblasts for 1 week, a period sufficient to induce functional maturation of mast cells (11)(12)(13)(14), IgE/Ag-stimulated release of ␤-HEX (Fig. 2G) (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Culture and Activation of BMMCs, Macrophages, and Dendritic Cells-To prepare BMMCs, bone marrow cells were obtained from femurs and tibias of mice and cultured in IL-3-containing BMMC complete medium consisting of DMEM, 10% FBS, 2 mM L-glutamine, 100 IU/ml penicillin, 100 g/ml streptomycin, 100 mM nonessential amino acids, and an optimal amount of recombinant mouse IL-3 that had been produced by the baculovirus/insect cell system, as described previously (11)(12)(13)(14). After 4 -5 weeks of culture, the majority of floating cells was confirmed to be immature BMMCs as assessed by Alcian blue-positive and safranin O-negative staining of their granules.…”

Section: Methodsmentioning

confidence: 99%

“…Maturation of immature BMMCs toward CTMC-like cells was described previously in detail (11)(12)(13)(14). Briefly, 5 ϫ 10 6 BMMCs were seeded on a subconfluent Swiss 3T3 fibroblast monolayer in 100-mm culture dishes and cocultured for up to 4 days in the presence of the baculovirus/insect cell-produced soluble form of SCF with replacement of the medium every 2 days.…”

Section: Methodsmentioning

confidence: 99%

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Analysis of Two Major Intracellular Phospholipases A2 (PLA2) in Mast Cells Reveals Crucial Contribution of Cytosolic PLA2α, Not Ca2+-independent PLA2β, to Lipid Mobilization in Proximal Mast Cells and Distal Fibroblasts

Ueno

Taketomi

Yamamoto

et al. 2011

Journal of Biological Chemistry

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Background: Mast cells express cPLA 2 ␣ and iPLA 2 ␤. Results: Knock-out of cPLA 2 ␣, not iPLA 2 ␤, hampers arachidonic acid mobilization in mast cells and adjacent fibroblasts. Conclusion: Mast cell cPLA 2 ␣ is coupled with stromal synthesis of anti-allergic PGE 2 , whereas iPLA 2 ␤ is dispensable for mast cell function. Significance: The cPLA 2 ␣-dependent transcellular PGE 2 synthesis opens new insight into the lipid biochemistry and mast cell biology fields.

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“…The gene is known to be involved in cellular differentiation [6,7], proliferation and growth arrest [6], heavy metal response [3,8], DNA damage [4], hypoxia response [8], tumor progression and metastasis [9,10]. The role of NDRG1 in tumor progression, invasion and metastasis remains somewhat enigmatic and controversial.…”

Section: Introductionmentioning

confidence: 99%

Equivocal Differential Effect of NDRG1 in Human Ovarian Cancer Cells

Napso¹,

Zino²,

Azzam³

et al. 2013

JCT

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Inactivation of tumor suppressor genes is a key factor in cancer regulation. N-myc downstream regulated gene 1 (NDRG1) is a tumor suppressor gene well known to be involved in carcinogenesis of numerous cancer types. The present study was designed to investigate the role of NDRG1 in human ovarian cancer, using SKOV-3 and SW626 (moderately and well differentiated cancer cells, respectively). Our results revealed that over-expressed NDRG1 significantly up-regulated the differentiation marker p21, in the ovarian cancer cell lines. This regulation led to decrease in cell viability and DNA synthesis rates in SW626 cells (83% and 89.5%, respectively). However, no effect on viability or on DNA synthesis was observed in SKOV-3 NDRG1-transfected cells. These findings prove that NDRG1 is indubitably functional in human ovarian cancer cells, as it up-regulated p21 expression. Nevertheless, this regulation showed differential effect on cell viability and DNA formation thus promoting the perception that downstream regulation of p21 could be inefficient in some cancer cells, a concept that needs to be further explored in order to understand its disability to play as regulator of cell cycle progression.

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Identification of NDRG1 as an early inducible gene during in vitro maturation of cultured mast cells

Cited by 48 publications

References 28 publications

Correlation of N-myc downstream-regulated gene 1 overexpression with progressive growth of colorectal neoplasm

Correlation of N-myc downstream-regulated gene 1 overexpression with progressive growth of colorectal neoplasm

Analysis of Two Major Intracellular Phospholipases A2 (PLA2) in Mast Cells Reveals Crucial Contribution of Cytosolic PLA2α, Not Ca2+-independent PLA2β, to Lipid Mobilization in Proximal Mast Cells and Distal Fibroblasts

Equivocal Differential Effect of NDRG1 in Human Ovarian Cancer Cells

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