Identification of NBS-encoding genes linked to black rot resistance in cabbage (Brassica oleracea var. capitata)

Afrin, Khandker Shazia; Rahim, Abdur; Park, Jong-In; Natarajan, Sathishkumar; Kim, Hoy‐Taek; Nou, Ill–Sup

doi:10.1007/s11033-018-4217-5

Cited by 30 publications

(24 citation statements)

References 60 publications

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“…Finally, three NBS-encoding genes ( Bo1010559 , Bo129866 and Bo1042121 ) were recently shown to function in resistance to black rot in cabbage ( Brassica oleracea var. capitata ), with higher expression levels detected in the resistant line compared to the susceptible lines [43].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Characterization of NBS-Encoding Genes in Watermelon and Their Potential Association with Gummy Stem Blight Resistance

Hassan

Rahim

Jung

et al. 2019

IJMS

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Watermelon (Citrullus lanatus) is a nutritionally rich and economically important horticultural crop of the Cucurbitaceae family. Gummy stem blight (GSB) is a major disease of watermelon, which is caused by the fungus Didymella bryoniae, and results in substantial economic losses in terms of yield and quality. However, only a few molecular studies have focused on GSB resistance in watermelon. Nucleotide binding site (NBS)-encoding resistance (R) genes play important roles in plant defense responses to several pathogens, but little is known about the role of NBS-encoding genes in disease resistance in watermelon. The analyzed NBS-encoding R genes comprises several domains, including Toll/interleukin-1 receptor(TIR), NBS, leucine-rich repeat (LRR), resistance to powdery mildew8(RPW8) and coiled coil (CC), which are known to be involved in disease resistance. We determined the expression patterns of these R genes in resistant and susceptible watermelon lines at different time points after D. bryoniae infection by quantitative RT-PCR. The R genes exhibited various expression patterns in the resistant watermelon compared to the susceptible watermelon. Only six R genes exhibited consistent expression patterns (Cla001821, Cla019863, Cla020705, Cla012430, Cla012433 and Cla012439), which were higher in the resistant line compared to the susceptible line. Our study provides fundamental insights into the NBS-LRR gene family in watermelon in response to D. bryoniae infection. Further functional studies of these six candidate resistance genes should help to advance breeding programs aimed at improving disease resistance in watermelons.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Characterization of NBS-Encoding Genes in Watermelon and Their Potential Association with Gummy Stem Blight Resistance

Hassan

Rahim

Jung

et al. 2019

IJMS

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“…Furthermore, RCY1, an LRR class R protein, confers resistance to Cucumber Mosaic Virus in Arabidopsis [ 29 , 33 ]. A recent study of NBS-encoding R genes in heading cabbage found that expression of some candidate R genes was significantly higher in black rot-resistant lines when compared to susceptible lines [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Gummy Stem Blight Resistance in Melon: Inheritance Pattern and Development of Molecular Markers

Hassan

Rahim

Natarajan

et al. 2018

IJMS

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Gummy stem blight (GSB) causes enormous losses to melon (Cucumis melo L.) production worldwide. We aimed to develop useful molecular markers linked to GSB resistance. In this study, 168 F2 plants were obtained from the F1 population of a cross between the GSB-susceptible ‘Cornell ZPPM 339’ and the GSB-resistant ‘PI482399’ lines. A 3:1 ratio of susceptible and resistant genotypes was observed in the F2 population, indicating control by a single recessive gene. Nucleotide-binding site leucine-rich repeat (NBS-LRR) genes confer resistance against insects and diseases in cucurbits including melon. We cloned and sequenced the TIR-NBS-LRR-type resistance gene MELO3C022157, located on melon chromosome 9, from resistant and susceptible lines. Sequence analysis revealed deletions in the first intron, a 2-bp frameshift deletion from the second exon and a 7-bp insertion in the 4th exon of the resistant line. We developed two insertion/deletion (InDel) markers, GSB9-kh-1 and GSB9-kh-2, which were found in the first intron of MELO3C022157 linked to GSB resistance. We validated these markers with the F2 population and inbred lines. These InDels may be used to facilitate marker-assisted selection of GSB resistance in melon. However, functional analysis of overexpressing and/or knock-down mutants is needed to confirm the frameshift mutation.

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“…To date, no BRR gene has been cloned from cruciferous vegetables (Singh et al, ). Some nucleotide‐binding site (NBS) genes have been identified in cabbage and radish by QTL mapping and comparative transcriptomics (Afrin et al, ; Lee et al, ; Wang, Song, et al, ); however, whether these genes are associated with BRR remains unknown. In radish, we need to further fine map the resistance genes to different races, develop more usable markers for MAS and verify gene function in future studies for improving the persistence of BRR, although we have discovered some important QTLs of BRR in this study.…”

Section: Discussionmentioning

confidence: 99%

Construction of high‐density linkage map and identification of QTLs associated with resistance to black rot in radish (Raphanus sativus) by RAD sequencing

Yang

Yundan

et al. 2020

Plant Breeding

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High‐density marker‐based QTL mapping can serve as an effective strategy to identify novel genomic information to facilitate crop improvement. In this study, we genotyped an F2 population (KB12‐1 × PP12‐1) using a RAD‐seq approach and constructed a high‐density linkage map for radish. After a series of filtering procedures were performed, 17,124 SNPs and 3,336 indels with aa × bb genotyping were retained to obtain bin markers. Then, a linkage map comprising a total of 1,221 bin markers in nine linkage groups spanning 1,467.3 cM with an average marker interval of 1.2 cM was constructed. We evaluated the resistance of the F2 mapping population to black rot using F3 progeny, and two major QTLs related to black rot resistance were identified based on this map. Among these QTLs, qBRR2 on Chr.2 explained 26.97% of the phenotypic variation with a LOD score of 11.93, and qBRR7 on Chr.7 accounted for 27.06% of the phenotypic variation with a LOD score of 11.83. The additive effect of qBRR2 was positive (14.97); however, qBRR7 had the opposite effect (−11.99). The high‐density linkage map and the major QTLs qBRR2 and qBRR7 provide new important information for disease resistance gene discovery and utilization in genetic improvement.

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Identification of NBS-encoding genes linked to black rot resistance in cabbage (Brassica oleracea var. capitata)

Cited by 30 publications

References 60 publications

Genome-Wide Characterization of NBS-Encoding Genes in Watermelon and Their Potential Association with Gummy Stem Blight Resistance

Genome-Wide Characterization of NBS-Encoding Genes in Watermelon and Their Potential Association with Gummy Stem Blight Resistance

Gummy Stem Blight Resistance in Melon: Inheritance Pattern and Development of Molecular Markers

Construction of high‐density linkage map and identification of QTLs associated with resistance to black rot in radish (Raphanus sativus) by RAD sequencing

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