Identification of mycobionts in an achlorophyllous orchid, Cremastra aphylla (Orchidaceae), based on molecular analysis and basidioma morphology

Yagame, Takahiro; Funabiki, Eriko; Yukawa, Tomohisa; Nagasawa, Eiji

doi:10.1016/j.myc.2017.08.001

Cited by 9 publications

(13 citation statements)

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“…The genus Coprinellus, also saprophytic fungi, was first described as very frequently associated with the terrestrial orchid Epipogium roseum by Yamato et al (2005), they suggest the existence of a highly specific relationship between this orchid species and members of the Psathyrellaceae family, owing to the high abundance of pelotons observed in their roots. Yagame et al (2013) confirmed the mycorrhizal status of Coprinellus by symbiotic cultivation of seeds from Cremastra appendiculata, a photosynthetic terrestrial orchid, and confirmed the presence and mycorrhiza formation by Coprinellus in Cremastra aphylla (Yagame et al 2018). Isolates belonging to this genus were detected very frequently in our study, a result which also confirm previous findings by Salazar et al (2020).…”

Section: Orchid Species Description-pleurothallis Coriacardiasupporting

confidence: 90%

Root endophytic fungi promote in vitro seed germination in Pleurothallis coriacardia (Orchidaceae)

et al. 2020

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Seeds of many orchids rely on the presence of fungi to trigger the germination process and even to initiate the full development of protocorms. While attention has been directed towards the study of mycorrhizal fungi, the diversity and functions of endophytic fungi from orchid roots remain underexplored, and few studies have verified their ecological role. This is the case of Pleurothallis coriacardia, an endemic green Neotropical orchid with both epiphytic and lithophytic habits growing in High-Andean montane forests. In the present study, we screened the cultivable fraction of the endophytic fungi colonizing the roots of mature plants of P. coriacardia using ITS rDNA markers. We also tested the potential of these endophytic fungi to improve embryo development and seed germination. Most of the isolated endophytes were classified within Psathyrellaceae. Some isolates, identified as members of the Ilyonectria and Coprinellus genera, significantly promoted embryo development in vitro in P. coriacardia seeds, a result that highlights the ecological roles these endophytic fungi may play in nature. Keywords: Andean montane forest, embryo development, epiphytic orchids, Ilyonectria, lithophytic orchids, Psathryrellaceae

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Section: Orchid Species Description-pleurothallis Coriacardiasupporting

confidence: 90%

Root endophytic fungi promote in vitro seed germination in Pleurothallis coriacardia (Orchidaceae)

et al. 2020

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Section: Diversity Of Mycobionts In Sap-mhpsmentioning

confidence: 99%

“…Lange (Yagame et al 2008 ) and Coprinellus domesticus (Bolton) Vilgalys, Hopple & Jacq. Johnson (Yagame et al 2018 ), respectively, by sporocarp morphology and molecular identification. Besides Agaricales fungi, SAP symbionts include wood-decay fungi from other Basidiomycota orders.…”

Section: Diversity Of Mycobionts In Sap-mhpsmentioning

confidence: 99%

“…Epipogium roseum was reported to be an SAP-MHP (Yamato et al 2005). Eulophia and Cremastra contain both leafy and leafless species, and SAP-MH was reported for both Eulophia zollingeri (Ogura-Tsujita and Yukawa 2008;Suetsugu et al 2020b) and C. aphyllla (Yagame et al 2018). The mycoheterotrophic genus Yoania includes four species, three of which are SAP-MHPs (Suetsugu et al 2020b;Yamashita et al 2020).…”

Section: Phylogeny and Evolution Of Sap-mhpsmentioning

confidence: 99%

“…Because of their saprotrophic nature, mycobionts of MHPs isolated from plant roots are amenable to pure culture, facilitating the identification of fungal species. Fungal isolates often develop basidiocarps on culture medium and can be identified morphologically (Burgeff 1932;Kikuchi et al 2008a;Terashita and Chuman 1987;Xu and Guo 2000;Yagame et al 2008Yagame et al , 2018. Most leaf-litter-decaying fungi that associate with MHPs belong to families in Agaricales, such as Mycenaceae, Marasmiaceae, and Omphalotaceae, which are the main fungal partners of Gastrodia and Didymoplexis (Table 1).…”

Section: Diversity Of Mycobionts In Sap-mhpsmentioning

confidence: 99%

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Evolutionary histories and mycorrhizal associations of mycoheterotrophic plants dependent on saprotrophic fungi

2021

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Mycoheterotrophic plants (MHPs) are leafless, achlorophyllous, and completely dependent on mycorrhizal fungi for their carbon supply. Mycorrhizal symbiosis is a mutualistic association with fungi that is undertaken by the majority of land plants, but mycoheterotrophy represents a breakdown of this mutualism in that plants parasitize fungi. Most MHPs are associated with fungi that are mycorrhizal with autotrophic plants, such as arbuscular mycorrhizal (AM) or ectomycorrhizal (ECM) fungi. Although these MHPs gain carbon via the common mycorrhizal network that links the surrounding autotrophic plants, some mycoheterotrophic lineages are associated with saprotrophic (SAP) fungi, which are free-living and decompose leaf litter and wood materials. Such MHPs are dependent on the forest carbon cycle, which involves the decomposition of wood debris and leaf litter, and have a unique biology and evolutionary history. MHPs associated with SAP fungi (SAP-MHPs) have to date been found only in the Orchidaceae and likely evolved independently at least nine times within that family. Phylogenetically divergent SAP Basidiomycota, mostly Agaricales but also Hymenochaetales, Polyporales, and others, are involved in mycoheterotrophy. The fungal specificity of SAP-MHPs varies from a highly specific association with a single fungal species to a broad range of interactions with multiple fungal orders. Establishment of symbiotic culture systems is indispensable for understanding the mechanisms underlying plant–fungus interactions and the conservation of MHPs. Symbiotic culture systems have been established for many SAP-MHP species as a pure culture of free-living SAP fungi is easier than that of biotrophic AM or ECM fungi. Culturable SAP-MHPs are useful research materials and will contribute to the advancement of plant science.

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Mycobiont identity and light conditions affect belowground morphology and physiology of a mixotrophic orchid Cremastra variabilis (Orchidaceae)

Yagame,

Figura,

Tanaka

et al. 2024

Mycorrhiza

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Identification of mycobionts in an achlorophyllous orchid, Cremastra aphylla (Orchidaceae), based on molecular analysis and basidioma morphology

Cited by 9 publications

References 13 publications

Root endophytic fungi promote in vitro seed germination in Pleurothallis coriacardia (Orchidaceae)

Root endophytic fungi promote in vitro seed germination in Pleurothallis coriacardia (Orchidaceae)

Evolutionary histories and mycorrhizal associations of mycoheterotrophic plants dependent on saprotrophic fungi

Mycobiont identity and light conditions affect belowground morphology and physiology of a mixotrophic orchid Cremastra variabilis (Orchidaceae)

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