Identification of Mycobacterium tuberculosis genes preferentially expressed during human infection

Kumar, Manoj; Khan, Farrah; Sharma, Sandeep; Kumar, Rahul; Faujdar, Jaya; Sharma, Rahul; Chauhan, D. S.; Singh, Rajinder; Magotra, Shyam K.; Khan, Inshad Ali

doi:10.1016/j.micpath.2010.10.003

Cited by 40 publications

(27 citation statements)

References 35 publications

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“…The plcABC locus, described as a probable virulence factor (47), is known as a preferential site for IS6110 transposition (48). Confirming this finding, the Zaragoza strain has three IS6110 copies, in plcC (Rv2349c), PPE38 (Rv2352c), and MT2423 (RvD4 region), within a region of around 10 kb.…”

Section: Discussionmentioning

confidence: 60%

Global Study of IS 6110 in a Successful Mycobacterium tuberculosis Strain: Clues for Deciphering Its Behavior and for Its Rapid Detection

et al. 2013

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eThe Mycobacterium tuberculosis insertion sequence IS6110, besides being a very useful tool in molecular epidemiology, seems to have an impact on the biology of bacilli. In the present work, we mapped the 12 points of insertion of IS6110 in the genome of a successful strain named M. tuberculosis Zaragoza (which has been referred to as the MTZ strain). This strain, belonging to principal genetic group 3, caused a large unsuspected tuberculosis outbreak involving 85 patients in Zaragoza, Spain, in 2001 to 2004. The mapping of the points of insertion of IS6110 in the genome of the Zaragoza strain offers clues for a better understanding of the adaptability and virulence of M. tuberculosis. Surprisingly, the presence of one copy of IS6110 was found in Rv2286c, as was recently described for a successful Beijing sublineage. As a result of this analysis, a rapid method for detecting this particular M. tuberculosis strain has been designed.

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Section: Discussionmentioning

confidence: 60%

Global Study of IS 6110 in a Successful Mycobacterium tuberculosis Strain: Clues for Deciphering Its Behavior and for Its Rapid Detection

et al. 2013

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“…The ribosome plays a major role in determining the overall gene expression profile of the cell (Starosta et al, 2014). Meanwhile, glyS (−2.51-fold) encoding a glycyl-tRNA synthetase has been suggested to be involved in DNA stabilization under extreme environmental conditions (Kumar et al, 2011). It has been previously shown that alterations of genes involved in translation can provoke slower growth of E .…”

Section: Discussionmentioning

confidence: 99%

The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of Zoonotic Streptococcus suis

Sun

Tan

Dong

et al. 2017

Front. Cell. Infect. Microbiol.

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Like eukaryotes, bacteria express one or more serine/threonine kinases (STKs) that initiate diverse signaling networks. The STK from Streptococcus suis is encoded by a single-copy stk gene, which is crucial in stress response and virulence. To further understand the regulatory mechanism of STK in S. suis, a stk deletion strain (Δstk) and its complementary strain (CΔstk) were constructed to systematically decode STK characteristics by applying whole transcriptome RNA sequencing (RNA-Seq) and phosphoproteomic analysis. Numerous genes were differentially expressed in Δstk compared with the wild-type parental strain SC-19, including 320 up-regulated and 219 down-regulated genes. Particularly, 32 virulence-associated genes (VAGs) were significantly down-regulated in Δstk. Seven metabolic pathways relevant to bacterial central metabolism and translation are significantly repressed in Δstk. Phosphoproteomic analysis further identified 12 phosphoproteins that exhibit differential phosphorylation in Δstk. These proteins are associated with cell growth and division, glycolysis, and translation. Consistently, phenotypic assays confirmed that the Δstk strain displayed deficient growth and attenuated pathogenicity. Thus, STK is a central regulator that plays an important role in cell growth and division, as well as S. suis metabolism.

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“…[9] used a genome-wide siRNA screen to identify 287 host factors that impact influenza A replication. Host cell molecules responsible for Mycobacterium tuberculosis intracellular survival and regulation of bacterial load in macrophages were identified using large-scale siRNA screens [10], [11]. A genome-wide siRNA screen identified 162 genes important for growth and persistence of the parasite Trypanosoma cruzi [12].…”

Section: Introductionmentioning

confidence: 99%

A Genome-Wide siRNA Screen to Identify Host Factors Necessary for Growth of the Parasite Toxoplasma gondii

2013

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Toxoplasma gondii is an obligate intracellular parasite that is able to infect virtually any nucleated cell of all warm-blooded animals. The host cell factors important for parasite attachment, invasion, and replication are poorly understood. We screened a siRNA library targeting 18,200 individual human genes in order to identify host proteins with a role in T. gondii growth. Our screen identified 19 genes whose inhibition by siRNA consistently and significantly lowered parasite replication. The gene ontology categories for those 19 genes represented a wide variety of functions with several genes implicated in regulation of the cell cycle, ion channels and receptors, G-protein coupled receptors, and cytoskeletal structure as well as genes involved in transcription, translation and protein degradation. Further investigation of 5 of the 19 genes demonstrated that the primary reason for the reduction in parasite growth was death of the host cell. Our results suggest that once T. gondii has invaded and established an infection, global changes in the host cell may be necessary to reduce parasite replication. While siRNA screens have been used, albeit rarely, in other parasite systems, this is the first report to describe a high-throughput siRNA screen for host proteins that affect T. gondii replication.

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Identification of Mycobacterium tuberculosis genes preferentially expressed during human infection

Cited by 40 publications

References 35 publications

Global Study of IS 6110 in a Successful Mycobacterium tuberculosis Strain: Clues for Deciphering Its Behavior and for Its Rapid Detection

Global Study of IS 6110 in a Successful Mycobacterium tuberculosis Strain: Clues for Deciphering Its Behavior and for Its Rapid Detection

The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of Zoonotic Streptococcus suis

A Genome-Wide siRNA Screen to Identify Host Factors Necessary for Growth of the Parasite Toxoplasma gondii

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