Identification of mutations in porcine STAT5A that contributes to the transcription of CISH

Abstract

Identification of causative genes or genetic variants associated with phenotype traits benefits the genetic improvement of animals. CISH plays a role in immunity and growth, however, the upstream transcriptional factors of porcine CISH and the genetic variations in these factors remain unclear. In this study, we firstly identified the minimal core promoter of porcine CISH and confirmed the existence of STATx binding sites. Overexpression and RT-qPCR demonstrated STAT5A increased CISH transcriptional activity (P < 0.01) and mRNA expression (P < 0.01), while GATA1 inhibited CISH transcriptional activity (P < 0.01) and the following mRNA expression (P < 0.05 or P < 0.01). Then, the putative functional genetic variations of porcine STAT5A were screened and a PCR-SSCP was established for genotype g.508A>C and g.566C>T. Population genetic analysis showed the A allele frequency of g.508A>C and C allele frequency of g.566C>T was 0.61 and 0.94 in Min pigs, respectively, while these two alleles were fixed in the Landrace population. Statistical analysis showed that Min piglets with CC genotype at g.566C>T or Hap1: AC had higher 28-day body weight, 35-day body weight, and ADG than TC or Hap3: CT animals (P < 0.05, P < 0.05). Further luciferase activity assay demonstrated that the activity of g.508A>C in the C allele was lower than the A allele (P < 0.05). Collectively, the present study demonstrated that STAT5A positively regulated porcine CISH transcription, and SNP g.566C>T in the STAT5A was associated with the Min piglet growth trait.

Strand-Specific RNA Sequencing Reveals Gene Expression Patterns in F1 Chick Breast Muscle and Liver after Hatching

“…Among these, STAT5A has been linked to cell specification, proliferation, differentiation, and survival at the molecular level [42]. Additionally, STAT5A has been found to play a role in immunity and growth by positively regulating porcine CISH gene transcription, and the SNP (g.566C > T) of STAT5A was associated with the piglet growth trait [43]. TGFB2 is a critical ligand in the TGF-β signaling pathway [44].…”

Evaluation of potential genetic marker for growth and carcass traits in Sumba Ongole (Bos indicus) cattle

“…Animal growth parameters are important in livestock breeding programs. Some hormones that are associated with animal growth have been reported, including growth hormone receptor (GHR) [ 1 ], growth hormone-releasing hormone (GHRH) [ 2 ], pituitary-specific transcription factor-1 (PIT-1) [ 3 ], and signal transducer and activator of transcription 5A (STAT5A) [ 4 ]. The GHR acts as a transmembrane protein, and its primary function is to bind GH with great specificity and affinity.…”