Identification of Multiresistant
            <i>Salmonella</i>
            Isolates Capable of Subsisting on Antibiotics

Barnhill, Alison E.; Weeks, Katherine E.; Xiong, Nalee; Day, Tim A.; Carlson, Steve A.

doi:10.1128/aem.02516-09

Cited by 24 publications

(24 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Limitation of bacterial growth is most commonly considered to result from nutrient deficiency; however, growth can also be influenced by diverse compounds that are inhibitory when abundant but serve as nutrients at lower concentrations (Abbott, 1973;Kunz et al, 1992;Alvarez et al, 2009;Barnhill et al, 2010). Organic acids, which are key intermediates in global carbon cycles (McInerney et al, 2009), exemplify this paradox in both natural and applied settings (Lee et al, 1976;Louis et al, 2007;Sousa et al, 2009;Huang et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Microbial mutualism dynamics governed by dose-dependent toxicity of cross-fed nutrients

et al. 2016

View full text Add to dashboard Cite

Microbial interactions, including mutualistic nutrient exchange (cross-feeding), underpin the flow of energy and materials in all ecosystems. Metabolic exchanges are difficult to assess within natural systems. As such, the impact of exchange levels on ecosystem dynamics and function remains unclear. To assess how cross-feeding levels govern mutualism behavior, we developed a bacterial coculture amenable to both modeling and experimental manipulation. In this coculture, which resembles an anaerobic food web, fermentative Escherichia coli and photoheterotrophic Rhodopseudomonas palustris obligately cross-feed carbon (organic acids) and nitrogen (ammonium). This reciprocal exchange enforced immediate stable coexistence and coupled species growth. Genetic engineering of R. palustris to increase ammonium cross-feeding elicited increased reciprocal organic acid production from E. coli, resulting in culture acidification. Consequently, organic acid function shifted from that of a nutrient to an inhibitor, ultimately biasing species ratios and decreasing carbon transformation efficiency by the community; nonetheless, stable coexistence persisted at a new equilibrium. Thus, disrupting the symmetry of nutrient exchange can amplify alternative roles of an exchanged resource and thereby alter community function. These results have implications for our understanding of mutualistic interactions and the use of microbial consortia as biotechnology.

show abstract

Section: Introductionmentioning

confidence: 99%

Microbial mutualism dynamics governed by dose-dependent toxicity of cross-fed nutrients

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Soil bacteria from the orders Pseudomonadales and Burkholderiales have also been isolated based on their capacity to grow on a range of antibiotics as a single carbon source (Dantas et al, 2008). In another environment including clinical and nonclinical samples, Barnhill et al (2011) observed that multi-resistant Salmonella spp. were also able to subsist on antibiotics, highlighting the potential prevalence of the antibiotic subsistence phenotype in a clinical context.…”

Section: Discussionmentioning

confidence: 99%

“…Several members of the bacterial community present in the environment have been identified as reservoirs of antibiotic resistance genes (Riesenfeld et al, 2004;D'Costa et al, 2006). In addition to resistance, recent studies showed that few bacterial species present in soil, seawater and gut microbiota of humans, farm and zoo animals are able to use antibiotics as a sole carbon source, known as the subsistence phenotype (Barnhill et al, 2011, Dantas et al, 2008Dopazo et al, 1988;Xin et al, 2012, chapter 2 of this thesis). In chapter 2, different approaches were implemented to study the genetic determinants involved in this phenotype.…”

Section: Subsistence Phenotype: An Ecological Contextmentioning

confidence: 99%

“…This phenotype is commonly referred to as "antibiotic subsistence" (Dopazo et al, 1988;Dantas et al, 2008;Barnhill et al, 2011;Xin et al, 2012). In addition, the concept of bacteria subsisting on antibiotics has been referred to as "antibioticresistant extremophiles" (Gabani et al, 2012) or "antibiotrophs" (Woappi et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Recent work has shown that a large and diverse group of bacteria from soil, seawater and the gut microbiota from humans and farm animals was not only able to resist the toxic effects of antibiotics, but also they could utilize antibiotics as a sole carbon source, a phenotype commonly referred to as antibiotic subsistence (Dopazo et al, 1988;Dantas et al, 2008;Barnhill et al, 2011;Xin et al, 2012). Controversially, Walsh et al (2013) showed that soil bacteria could not utilize antibiotics as a carbon source since no degradation of antibiotics occurred.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Interplay between gut microbiota and antibiotics

Gonzalez¹

View full text Add to dashboard Cite

The human body is colonized by a vast number of microorganisms collectively defined as the microbiota. In the gut, the microbiota has important roles in health and disease, and can serve as a host of antibiotic resistance genes. Disturbances in the ecological balance, e.g. by antibiotics, can affect the diversity and dynamics of the microbiota. The extent of the disturbance induced by antibiotics is influenced by, among other factors, the class of antibiotic, the dose, and administration route. One of the most common consequences of excessive antibiotic use is the emergence of antibiotic resistant bacteria and the dissemination of the corresponding resistance genes to other microbial inhabitants of the gut community, in addition to affecting the colonization resistance and promoting the overgrowth of pathogens. These effects are particularly relevant for Intensive Care Unit (ICU) patients, which are frequently exposed to a high risk of hospital-acquired infections associated with antibiotic resistant bacteria.Due to the important roles that members of the gut microbiota play in the host, including their role as potential hubs for the dissemination of antibiotic resistance, recent research has focused on determining the composition and function of gut microorganisms and the antibiotic resistance genes associated with them.The objectives of the research described in this thesis were to study the diversity and dynamics of the gut microbiota and resistome in ICU patients receiving antibiotic prophylactic therapy, and to assess the colonization dynamics with antibiotic resistant bacteria focusing on the commensal microbiota as a reservoir of antibiotic resistance genes by using culture dependent and independent techniques. Furthermore, the genetic background involved in the subsistence phenotype was investigated to disentangle the links between resistance and subsistence.Bacteria harbor antibiotic resistance genes that participate in a range of processes such as resisting the toxic effects of antibiotics, but could also aid in the utilization of antibiotics as sole carbon source, referred to as antibiotic subsistence phenotype.In chapter 2, the potential of gut bacteria from healthy human volunteers and zoo animals to subsist on antibiotics was investigated.Various gut isolates of Escherichia coli and Cellulosimicrobium spp. displayed the subsistence phenotype, mainly with aminoglycosides. Although no antibiotic degradation could be detected, the number of colony forming units increased during growth in medium with only the antibiotic as a carbon source. By using different approaches to study the aminoglycoside subsistence phenotype, we observed that laboratory strains carrying the aminoglycoside 3'phosphotransferase II gene also displayed the subsistence phenotype on aminoglycosides and that glycosylhydrolases seem to be involved in the subsistence phenotype. As the zoo animals for which the subsistence phenotype was investigated also included a number of nonhuman primates, the applicability of Human Intesti...

show abstract

Ecological and Clinical Consequences of Antibiotic Subsistence by Environmental Microbes

Dantas¹,

Sommer²

2011

Antimicrobial Resistance in the Environment

View full text Add to dashboard Cite

Identification of Multiresistant Salmonella Isolates Capable of Subsisting on Antibiotics

Cited by 24 publications

References 9 publications

Microbial mutualism dynamics governed by dose-dependent toxicity of cross-fed nutrients

Microbial mutualism dynamics governed by dose-dependent toxicity of cross-fed nutrients

Interplay between gut microbiota and antibiotics

Ecological and Clinical Consequences of Antibiotic Subsistence by Environmental Microbes

Contact Info

Product

Resources

About