Identification of Multiple Low-Level Resistance Determinants and Coselection of Motility Impairment upon Sub-MIC Ceftriaxone Exposure in Escherichia coli

Ching, Carly; Zaman, Muhammad H

doi:10.1128/msphere.00778-21

Cited by 11 publications

(7 citation statements)

References 67 publications

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“…7 Regarding the assay results, underdosed antibiotics could be responsible for therapy failures and antibiotic resistance, which can lead to the reduction or absence of effectiveness of first-line therapies. 5,[35][36][37][38] The WHO has recently reported high resistance levels for Escherichia coli and Salmonella spp. to ciprofloxacin.…”

Section: Prevalence Of Sf Medicines and Cause Of Noncompliancementioning

confidence: 99%

Prevalence of Poor Quality Ciprofloxacin and Metronidazole Tablets in Three Cities in Cameroon

Tchounga

Sacré

Hamuli

et al. 2023

The American Journal of Tropical Medicine and Hygiene

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Quality is one of the essential components of medicines and needs to be ensured to preserve the population’s health. This can be achieved through post-marketing quality control of medicines and is one of the most important duties of national regulatory authorities. In collaboration with the Cameroonian National Drug Quality Control and Valuation Laboratory, the decision was made to initiate a prevalence study to assess the quality of antiinfective medicines in Cameroon. A total of 150 samples of ciprofloxacin tablets and 142 samples of metronidazole tablets were collected from 76 licensed pharmacies and 75 informal vendors in three cities in Cameroon using a random strategy wherever possible and a mystery shopper approach. Three tests were carried out on each of the samples. Visual inspection allowed to find two falsified samples (0.7%) due to lack of information about the manufacturing company, and five more samples (1.7%) were deemed to be substandard due to flaws in the product. An additional 13 samples (4.5%) failed disintegration testing, and six (2.1%) others failed high-performance liquid chromatography assay testing due to insufficient active pharmaceutical ingredient (API) content. All samples were found to contain some API. A prevalence of 7.9% substandard or falsified (SF) medicines was found. Moreover, the prevalence of outlets selling SF medicines was greater in the informal sector (26.7%) than in the formal sector (2.6%). Although the prevalence of SF medicines found was low, efforts need to be made by national regulatory authorities to monitor the pharmaceutical market more closely.

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Section: Prevalence Of Sf Medicines and Cause Of Noncompliancementioning

confidence: 99%

Prevalence of Poor Quality Ciprofloxacin and Metronidazole Tablets in Three Cities in Cameroon

Tchounga

Sacré

Hamuli

et al. 2023

The American Journal of Tropical Medicine and Hygiene

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“…The OmpR showed the highest conservation among the proteins explored with 97% (233/239 sites) of conserved amino acid sites (Table S9 ). Two important sites (G63 and R150 24 , 25 ) were conserved in all genomes.…”

Section: Resultsmentioning

confidence: 99%

In-depth analysis of Klebsiella aerogenes resistome, virulome and plasmidome worldwide

Morgado,

Fonseca,

Freitas

et al. 2024

Sci Rep

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Klebsiella aerogenes is an emergent pathogen associated with outbreaks of carbapenem-resistant strains. To date, studies focusing on K. aerogenes have been small-scale and/or geographically restricted. Here, we analyzed the epidemiology, resistome, virulome, and plasmidome of this species based on 561 genomes, spanning all continents. Furthermore, we sequenced four new strains from Brazil (mostly from the Amazon region). Dozens of STs occur worldwide, but the pandemic clones ST93 and ST4 have prevailed in several countries. Almost all genomes were clinical, however, most of them did not carry ESBL or carbapenemases, instead, they carried chromosomal alterations (omp36, ampD, ampG, ampR) associated with resistance to β-lactams. Integrons were also identified, presenting gene cassettes not yet reported in this species (blaIMP, blaVIM, blaGES). Considering the virulence loci, the yersiniabactin and colibactin operons were found in the ICEKp10 element, which is disseminated in genomes of several STs, as well as an incomplete salmochelin cluster. In contrast, the aerobactin hypervirulence trait was observed only in one ST432 genome. Plasmids were common, mainly from the ColRNAI replicon, with some carrying resistance genes (mcr, blaTEM, blaNDM, blaIMP, blaKPC, blaVIM) and virulence genes (EAST1, senB). Interestingly, 172 genomes of different STs presented putative plasmids containing the colicin gene.

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“…Two previous studies of sub-MIC exposure to ciprofloxacin and ceftriaxone in E. coli have found that low dose antimicrobials selected for resistant isolates, but that in only a minority of isolates could the elevated MICs be explained by known resistance mechanisms [69,70]. Both studies found novel mutations that could explain the increased MICs.…”

Section: Discussionmentioning

confidence: 97%

Effect of Erythromycin Residuals in Food on the Development of Resistance in Streptococcus pneumoniae: An In Vivo Study in Galleria mellonella

Baranchyk,

Gestels,

Van Den Bossche

et al. 2024

Preprint

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BackgroundThe use of antimicrobials to treat food animals may result in antimicrobial residues in foodstuffs of animal origin. The European Medicines Association (EMA) and World Health Organization (WHO) define safe antimicrobial concentrations in food based on acceptable daily intakes (ADIs). It is unknown if ADI doses of antimicrobials in food could influence the antimicrobial susceptibility of human-associated bacteria. ObjectivesThis aim of this study was to evaluate if the consumption of ADI doses of erythromycin could select for erythromycin resistance in a Galleria mellonella model of Streptococcus pneumoniae infection. Methods A chronic model of S. pneumoniae infection in G. mellonella larvae was used for the experiment. Inoculation of larvae with S. pneumoniae was followed by injections of EMA (0.0875 μg/ml) and WHO (0.012 μg/ml) ADI doses of erythromycin proceeded by isolation of S. pneumoniae colonies on selective agar plates. Minimum inhibitory concentrations (MICs) of resistant colonies were measured, and whole genome sequencing (WGS) was performed followed by variant calling to determine the genetic modifications.   Results Exposure to single doses of both EMA and WHO ADI doses of erythromycin resulted in the emergence of erythromycin resistance in S. pneumoniae. Emergent resistance to erythromycin was associated with a mutation in rplA. Conclusion In our in vivo model, even single dose of erythromycin that are classified as acceptable by the WHO and EMA induced significant increases in erythromycin MICs in S. pneumoniae. These results suggest the need to include the induction of antimicrobial resistance (AMR) as a significant criterion for determining ADIs.

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Identification of Multiple Low-Level Resistance Determinants and Coselection of Motility Impairment upon Sub-MIC Ceftriaxone Exposure in Escherichia coli

Abstract: Ceftriaxone is a widely consumed antibiotic used to treat bacterial infections. Bacteria, however, are increasingly becoming resistant to ceftriaxone.

Cited by 11 publications

References 67 publications

Prevalence of Poor Quality Ciprofloxacin and Metronidazole Tablets in Three Cities in Cameroon

Prevalence of Poor Quality Ciprofloxacin and Metronidazole Tablets in Three Cities in Cameroon

In-depth analysis of Klebsiella aerogenes resistome, virulome and plasmidome worldwide

<strong></strong>Effect of Erythromycin Residuals in Food on the Development of Resistance in <em>Streptococcus pneumoniae</em>: An <em>In Vivo</em> Study in <em>Galleria mellonella</em>

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