Identification of multiple active growth factors in basement membrane matrigel suggests caution in interpretation of cellular activity related to extracellular matrix components

Vukičević, Slobodan; Kleinman, Hynda K.; Luyten, Frank P.; Roberts, Anita B.; Roche, Nanette S.; Reddi, A. Hari

doi:10.1016/0014-4827(92)90397-q

Cited by 596 publications

(399 citation statements)

References 33 publications

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“…Matrigel, a soluble basement matrix containing various bioactive substances secreted by Engelbreth-HolmSwan mouse sarcoma cells (Vukicevic et al 1992), has been reported to maintain the expression of hepatocyte-specific genes, including Albumin, in adult hepatocytes (Bissell et al, 1987;Ben-Ze'ev et al, 1988;Schuetz et al, 1988;Caron, 1990). Here, we found that culture of the epithelium on Matrigel led to their differentiation into hepatocytes (Figs.…”

Section: Ecm Components Are Important For the Differentiation Of The mentioning

confidence: 59%

“…Moreover, this model that Activin/TGF-␤ signaling is required for FGF-driven BEC differentiation in vivo could explain our observation that single administration of FGF was able to induce BEC differentiation in vitro. Probably, synergy between the administrated bFGF and pre-existing TGF-␤ in the culture medium (Matrigel, or serum is known to contain small amount of TGF-␤; Vukicevic et al, 1992;Masui et al, 1986) would be able to induce the hepatoblasts to differentiate into BEC in vitro.…”

Section: Fgf Signaling Is Insufficient To Induce Bile Duct Differentimentioning

confidence: 99%

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FGF signaling segregates biliary cell‐lineage from chick hepatoblasts cooperatively with BMP4 and ECM components in vitro

Yanai

Tatsumi

Hasunuma

et al. 2008

Developmental Dynamics

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Intrahepatic bile ducts (IHBDs) are indispensable for transporting bile secreted from hepatocytes to the hepatic duct. The biliary epithelial cells (BECs) of the IHBD arise from bipotent hepatoblasts around the portal vein, suggesting the portal mesenchyme is essential for their development. However, except for Notch or Activin/ TGF-␤ signaling molecules, it is not known which molecules regulate IHBD development. Here, we found that FGF receptors and BMP4 are specifically expressed in the developing IHBD and the hepatic mesenchyme, respectively. Using a mesenchyme-free culture of liver bud, we showed that bFGF and FGF7 induce the hepatoblasts to differentiate into BECs, and that BMP4 enhances bFGF-induced BEC differentiation. The extracellular matrix (ECM) components in the hepatic mesenchyme induced BEC differentiation. Forced expression of a constitutively active form of the FGF receptor partially induced BEC differentiation markers in vivo. These data strongly suggest that bFGF and FGF7 promote BEC differentiation cooperatively with BMP4 and ECMs in vivo.

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Section: Ecm Components Are Important For the Differentiation Of The mentioning

confidence: 59%

Section: Fgf Signaling Is Insufficient To Induce Bile Duct Differentimentioning

confidence: 99%

FGF signaling segregates biliary cell‐lineage from chick hepatoblasts cooperatively with BMP4 and ECM components in vitro

Yanai

Tatsumi

Hasunuma

et al. 2008

Developmental Dynamics

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“…Here, we discuss the uses of a tumor-derived basement membrane extract for assessment of cancer cell behavior and growth in vivo. This basement membrane extract (known as BME, Matrigel) was first isolated from a murine tumor as a crude protein mixture that was a liquid at 4 C and gelled under physiological conditions at 24-37 C. 1,4 It is composed mainly of laminin-111, collagen IV, heparan sulfate proteoglycan, various growth factors, and additional components (Table 1). These hydrogels contain all of the components found in many embryonic basement membranes; however, these components do not exhibit cross-linking as seen in an in vivo basement membrane.…”

Section: Introductionmentioning

confidence: 99%

“…These hydrogels contain all of the components found in many embryonic basement membranes; however, these components do not exhibit cross-linking as seen in an in vivo basement membrane. 4,10 This basement membrane-like matrix is nevertheless biologically active with various cell types, including cancer cells. When normal endothelial or epithelial cells were plated on the gelled basement membrane-like extract and then cultured under standard conditions, highly specific differentiation was observed with reduced proliferation.…”

Section: Introductionmentioning

confidence: 99%

Multiple uses of basement membrane‐like matrix (BME/Matrigel) in vitro and in vivo with cancer cells

Benton

Kleinman

George

et al. 2011

Intl Journal of Cancer

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184

134

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Significant advances in our understanding of cancer cell behavior, growth, and metastasis have been facilitated by studies using a basement membrane-like extracellular matrix extract, also known as Matrigel. The basement membrane is a thin extracellular matrix that is found in normal tissues and contacts epithelial and endothelial cells, smooth muscle, fat, Schwann cells, etc. It is composed of mainly laminin-111, collagen IV, heparan sulfate proteoglycan, entactin/nidogen, and various growth factors (fibroblast growth factor, transforming growth factor beta, epidermal growth factor, etc.). Most tumors of epithelial origin produce significant amounts of basement membrane matrix and interact with it particularly during metastasis. Cancer cells metastasize via degradation of the vessel basement membrane matrix to extravasate into the blood stream and colonize distant sites. This review will focus on the interaction of cancer cells and cancer stem cells with the basement membrane-like matrix and the various uses of this interaction to accelerate tumor growth in vivo and to develop in vitro assays for invasion, morphology, and dormancy. Such assays and methods have advanced our understanding of the process of cancer progression, the genes and pathways that are involved, the potential of various therapeutic agents, the effects of neighboring cells, and the role of stem cells.

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“…Type IV collagen is physiologicallyorganized into and specific for the 2-dimensional BM rather than the 3D ECM, thus Matrigel™ may be best considered as a BM rather than as an ECM product, per se. In addition, Matrigel™ contains several angiogenic-and growthpromoting factors, which may in part explain the enhanced tumor growth when Matrigel™ is used as a vehicle in nude mice tumorigenicity studies [72,73].…”

Section: In Vitro Models Of the Extracellular Matrix In Digestive Cancermentioning

confidence: 99%

The Extracellular Matrix in Digestive Cancer

Worthley

Giraud

Wang

2010

Cancer Microenvironment

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The extracellular components of the cancer microenvironment play a critical role in tumor initiation, progression and invasion. In this review we examine the normal formation and function of the basement membrane and extracellular matrix. We characterize the interactions between the matrix and the epithelium and explore the causes and consequences of the extracellular remodeling that accompanies carcinogenesis. Finally, we address the therapeutic possibilities of incorporating matrix as well as epithelial strategies in the management of digestive cancer.

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Identification of multiple active growth factors in basement membrane matrigel suggests caution in interpretation of cellular activity related to extracellular matrix components

Cited by 596 publications

References 33 publications

FGF signaling segregates biliary cell‐lineage from chick hepatoblasts cooperatively with BMP4 and ECM components in vitro

FGF signaling segregates biliary cell‐lineage from chick hepatoblasts cooperatively with BMP4 and ECM components in vitro

Multiple uses of basement membrane‐like matrix (BME/Matrigel) in vitro and in vivo with cancer cells

The Extracellular Matrix in Digestive Cancer

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