Identification of monocyte-related transcriptomic signature of peripheral blood mononuclear cells in type 1 diabetes

Yin, Min; Zhang, Yan; Hao, Juan; Li, Xinyu; Yu, Haibo; Li, Xia

doi:10.1097/cm9.0000000000002142

Cited by 3 publications

(3 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a result, IFNγ⁺ treatments in both paracrine and cell contact approaches substantially decreased TLR4 levels in two sources of PBMCs. Several studies have indicated that TLR4 levels are higher in uncultured PBMCs freshly isolated from T1D patients [68,69]. However, our results displayed a decrease in TLR4 levels of PBMCs compared to HC PBMCs across all treatments.…”

Section: Discussioncontrasting

confidence: 71%

Different paracrine and cell contact effects of human amniotic fluid-derived mesenchymal stem cells on peripheral blood mononuclear cells of type 1 diabetes patients imply the regulatory role of interferon-γ in their interactions

Hoseini,

Moghimi,

Hosseini

et al. 2023

Preprint

View full text Add to dashboard Cite

Besides the involvement in the pathogenesis of type 1 diabetes mellitus (T1DM), IFNγ is used to license mesenchymal stem cells (MSCs) for displaying immunomodulatory properties. Therefore, we aimed to investigate the interaction of MSCs in two preconditioned (IFNγ⁺) and non-preconditioned (IFNγ⁻) conditions, with PBMCs from healthy control (HC) and T1DM sources in the co-culture system and hypothesized differential induction of chemokines and their receptors, and T regulatory (Treg) cells due to the function of IFNγ. Our results confirmed that MSC/IFNγ⁺ and MSC/IFNγ⁻ treatments reciprocally respond to cell contact with HC and T1DM PBMCs differently, regarding the expression of anti-inflammatory genes (IDO1, IDO2, ICAM-1), chemokine ligands (CCL3, CXCL9, CXCL10) and receptors involved in immune cell trafficking(CXCR3, CXCR6, TLR4). Our results also demonstrated significant differences between HC and T1DM PBMCs by interaction with AF-MSCs (in IFNγ⁺ and IFNγ⁻ states) through paracrine and cell contact approaches regarding the expression of some target genes, including CXCR3 and its ligands (CXCL9 and CXCL10), CXCR6, CCR5and its ligands (CCL3 and CCL4). These differences were also reflected in the proportion of Treg cells in HC and T1DM samples. Therefore, the presence of regulatory roles of IFNγ in the pathogenesis of autoimmune diabetes seems inevitable.

show abstract

Section: Discussioncontrasting

confidence: 71%

Different paracrine and cell contact effects of human amniotic fluid-derived mesenchymal stem cells on peripheral blood mononuclear cells of type 1 diabetes patients imply the regulatory role of interferon-γ in their interactions

Hoseini,

Moghimi,

Hosseini

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Previous studies have shown that CD4 T cells co-cultured with MSCs upregulate TLR4 (Selleri et al 2013) which was re ected in our ndings. Former studies have disclosed the higher levels of TLR4 in freshly isolated PBMCs from T1D patients (Zhang et al 2020; Yin et al 2022). However, our results displayed a decrease in TLR4 levels of T1D PBMCs compared to HC PBMCs across all treatments.…”

Section: Discussionmentioning

confidence: 99%

The microenvironment resulting from the co-culture of human amniotic fluid-derived mesenchymal stem cells with peripheral blood mononuclear cells differs between type 1 diabetes mellitus patients and healthy individuals

Hoseini,

Moghimi,

Hosseini

et al. 2023

Preprint

View full text Add to dashboard Cite

IFNγ is one of the main factors involved in type 1 diabetes (T1D) pathogenesis and has also been used to license mesenchymal stem cells (MSCs) for displaying immunosuppressive properties in a process termed preconditioning/priming. Our study aimed to investigate the interaction of amniotic fluid-derived MSCs (AF-MSCs) in two preconditioned (IFNγ⁺) and non-preconditioned (IFNγ⁻) conditions, with peripheral blood mononuclear cells (PBMCs) from the sources of healthy control (HC) and T1D. Accordingly, the interactions were assessed through anti-inflammatory genes, chemokines and their receptors, plus the induction of T regulatory (Treg) cells. Our results demonstrated that MSC/IFNγ⁺ and MSC/IFNγ⁻ treatments respond conversely to HC and T1D PBMCs regarding the expression of anti-inflammatory genes (IDO1, IDO2, ICAM-1), chemokine ligands (CCL3, CXCL9, CXCL10) and receptors involved in immune cell trafficking (CXCR3, CXCR6, TLR4). Our findings also confirmed the same opposite effects of HC and T1D PBMCs when interacting with IFNγ⁺ and IFNγ⁻ MSCs regarding the expression of target genes, including CXCR3 and its ligands (CXCL9 and CXCL10), CXCR6, CCR5 and its ligands (CCL3 and CCL4). These differences were also reflected in the proportion of Treg cells in HC and T1D samples, depending on whether it was assessed through paracrine or cell contact approaches. Our research indicates that the interaction between IFNγ⁺ and IFNγ⁻ MSCs and T1D PBMCs creates distinct microenvironments compared to those in HC PBMCs. This implies that the intravenous administration of MSCs into T1D patients may result in different outcomes than in healthy individuals that can be manipulated by the preconditioning of MSCs.

show abstract

“…17,18 Monocyte subsets distribution is altered in T1D patients. [19][20][21] M1 macrophages have increased glycolysis and pentose phosphate activity, but suppress the TCA cycle, resulting in metabolite accumulation, while anti-inammatory M2 macrophages have a characteristic oxidative-metabolic prole with a high level of fatty acid oxidation. [22][23][24] The circulating prole of T lymphocytes is also altered in patients with T1D, 25,26 especially in patients with vascular complications of T1D, 18 who have a transition from naive T lymphocytes to effector cells, as well as signicant changes in the levels of T lymphocytes polarization and T lymphocytes effector cytokine.…”

mentioning

confidence: 99%

Prediction of type 1 diabetes with machine learning algorithms based on FTIR spectral data in peripheral blood mononuclear cells

Rostoka,

Shvirksts,

Salna

et al. 2023

Anal. Methods

View full text Add to dashboard Cite

show abstract

Identification of monocyte-related transcriptomic signature of peripheral blood mononuclear cells in type 1 diabetes

Cited by 3 publications

References 5 publications

Different paracrine and cell contact effects of human amniotic fluid-derived mesenchymal stem cells on peripheral blood mononuclear cells of type 1 diabetes patients imply the regulatory role of interferon-γ in their interactions

Different paracrine and cell contact effects of human amniotic fluid-derived mesenchymal stem cells on peripheral blood mononuclear cells of type 1 diabetes patients imply the regulatory role of interferon-γ in their interactions

The microenvironment resulting from the co-culture of human amniotic fluid-derived mesenchymal stem cells with peripheral blood mononuclear cells differs between type 1 diabetes mellitus patients and healthy individuals

Prediction of type 1 diabetes with machine learning algorithms based on FTIR spectral data in peripheral blood mononuclear cells

Contact Info

Product

Resources

About