Identification of molecular stress indicators in pikeperch Sander lucioperca correlating with rising water temperatures

Swirplies, Fabian; Wuertz, Sven; Baßmann, Björn; Orban, Axel; Schäfer, Nadine; Brunner, Ronald M.; Hadlich, Frieder; Goldammer, Tom; Rebl, Alexander

doi:10.1016/j.aquaculture.2018.11.043

Cited by 39 publications

(30 citation statements)

References 75 publications

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“…Likewise, the expression of the gene hsp90aa1 (alias hsp90-alpha, Heat Shock Protein 90 alpha) that codes for the inducible form of HSP90 and its constitutive counterpart hsp90ab1 (alias hsp90-beta, Heat Shock Protein 90 beta) were both up-regulated in WN and WH sh as compared to CT sh (although only hsp90aa1 was qPCR validated). These ndings are in line with previous studies reporting higher expression of hsp90aa1 mRNA, and minor effects on the constitutive expression of hsp90ab1, after thermal stress in sh [15,92]. HSP90AA1 is an abundant molecular chaperone and is implicated in a wide variety of cellular processes including protection of the proteome, the folding and transport of newly synthesized polypeptides, and the activation of proteolysis of misfolded proteins [88,93,94].…”

Section: Heat Shock Response Unfolded Protein Response and Endoplasmsupporting

confidence: 91%

“…Transcriptomic techniques allow for the high-throughput identi cation of genes that are sensitive to particular conditions, and can be used as biomarkers for the detection and quanti cation of stress levels and stress tolerance. In this context, the development of diagnostic biomarkers for quantifying the impact of environmental stressors on an organism's physiology and health has received increased attention by the aquaculture industry and for use in ecological surveys [89,92,151]. Our results agree with recent studies which show that the genes serpinh1, hsp90aa1 and cirbp are reliable molecular biomarkers for the detection and quanti cation of thermal stress in salmonids [89,92,152].…”

Section: Biomarker Genessupporting

confidence: 88%

“…Decreased expression of ucp2 transcript levels with increasing temperatures (15-25 °C) was previously reported in the gill and liver of pikeperch (Sander lucioperca) [92], and demonstrates that UCP2 has a thermogenic function [135]. For instance, gilthead sea bream (Sparus aurata) showed a signi cant decrease in the expression of ucp2 in the whole blood after acute (1 h) exposure to 18-20% oxygen saturation [136].…”

Section: Oxidative Stressmentioning

confidence: 58%

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The Transcriptomic Responses of Atlantic Salmon (Salmo salar) to High Temperature Stress Alone, and in Combination with Moderate Hypoxia

Beemelmanns

Zanuzzo

Xue

et al. 2020

Preprint

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Background: Increases in seawater temperatures and in the frequency and severity of hypoxic events are expected with climate change, and may become a challenge for cultured Atlantic salmon and negatively affect their growth, immunology and welfare. Thus, we examined how an incremental temperature increase alone (Warm & Normoxic-WN: 12→20°C; 1°C week-1), and in combination with moderate hypoxia (Warm & Hypoxic-WH: ~70% air saturation), impacted salmon’s hepatic transcriptome expression compared to control fish (CT: 12°C, normoxic) using 44K microarrays and qPCR. Results: Overall, we identified 2,894 differentially expressed probes (DEPs, FDR < 5%), that included 1,111 shared DEPs, while 789 and 994 DEPs were specific to WN and WH fish, respectively. Pathway analysis suggested that the cellular mechanisms affected by the two experimental conditions were quite similar, with up-regulated genes functionally associated with heat shock response, ER-stress, apoptosis and immune defence, while genes connected with general metabolic processes, proteolysis and oxidation-reduction were largely suppressed. The qPCR assessment of 41 microarray-identified genes validated that the heat shock response (hsp90aa1, serpinh1), apoptosis (casp8, jund, jak2) and immune responses (apod, c1ql2, epx) were up-regulated in WN and WH fish, while oxidative stress and hypoxia sensitive genes were down-regulated (cirbp, cyp1a1, egln2, gstt1, hif1α, prdx6, rraga, ucp2). However, the additional challenge of hypoxia resulted in more pronounced effects on heat shock and immune-related processes, including a stronger influence on the expression of 14 immune-related genes. Finally, robust correlations between the transcription of 19 genes and several phenotypic traits in WH fish suggest that changes in gene expression were related to an impaired physiological and growth performance. Conclusion: Increasing temperature to 20°C alone, and in combination with hypoxia, resulted in the up- and down-regulation of genes involved in similar important pathways in Atlantic salmon. However, the heat shock and immune responses of fish exposed to 20°C and hypoxia were more affected, and their transcriptional dysregulation was related to reduced performance. This study provides valuable information on how these two environmental challenges affect the expression of stress-, metabolic- and immune-related genes and pathways and identifies potential biomarker genes for improving our understanding of fish health and welfare.

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Section: Heat Shock Response Unfolded Protein Response and Endoplasmsupporting

confidence: 91%

Section: Biomarker Genessupporting

confidence: 88%

Section: Oxidative Stressmentioning

confidence: 58%

See 1 more Smart Citation

The Transcriptomic Responses of Atlantic Salmon (Salmo salar) to High Temperature Stress Alone, and in Combination with Moderate Hypoxia

Beemelmanns

Zanuzzo

Xue

et al. 2020

Preprint

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“…The brain of each maraena whitefish was dissected into the hypothalamus, telencephalon, and hindbrain and sampled together with the spleen, liver, white muscle, gills, head kidney (HK), and heart. Samples were snap-frozen in liquid nitrogen and stored at −80 • C. Husbandry and sampling of pikeperch and rainbow trout have been described elsewhere [21,22].…”

Section: Fish Husbandry and Experimental Treatmentmentioning

confidence: 99%

“…Fluidigm RealTime PCR Analysis Software (v. 3.0.2, https://www.fluidigm.com/software) was used to analyze the qPCR results. To obtain the relative copy number of each transcript, a serial dilution-based standard curve (10 2 -10 6 copies) was used and the copy number was normalized with the geometric mean of three suitable reference genes (EEF1A1b, RPL9, and RLP32 for maraena whitefish; EEF1A1, ACTB, and RPS5 for rainbow trout; and EEF1A1, RPS5, and RLP32 for pikeperch) [21,[26][27][28].…”

Section: Primer Design and Quantitative Pcrmentioning

confidence: 99%

Characterization of Sialic Acid-Binding Immunoglobulin-Type Lectins in Fish Reveals Teleost-Specific Structures and Expression Patterns

Bornhöfft

Ribera

Viergutz

et al. 2020

Cells

Self Cite

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The cellular glycocalyx of vertebrates is frequently decorated with sialic acid residues. These sialylated structures are recognized by sialic acid-binding immunoglobulin-type lectins (Siglecs) of immune cells, which modulate their responsiveness. Fifteen Siglecs are known to be expressed in humans, but only four Siglecs are regularly present in fish: Siglec1, CD22, myelin-associated glycoprotein (MAG), and Siglec15. While several studies have dealt with the physiological roles of these four Siglecs in mammals, little is known about Siglecs in fish. In the present manuscript, the expression landscapes of these Siglecs were determined in the two salmonid species Oncorhynchus mykiss and Coregonus maraena and in the percid fish Sander lucioperca. This gene-expression profiling revealed that the expression of MAG is not restricted to neuronal cells but is detectable in all analyzed blood cells, including erythrocytes. The teleostean MAG contains the inhibitory motif ITIM; therefore, an additional immunomodulatory function of MAG is likely to be present in fish. Besides MAG, Siglec1, CD22, and Siglec15 were also expressed in all analyzed blood cell populations. Interestingly, the expression profiles of genes encoding Siglecs and particular associated enzymes changed in a gene- and tissue-specific manner when Coregonus maraena was exposed to handling stress. Thus, the obtained data indicate once more that stress directly affects immune-associated processes.

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Recent progress in European percid fish culture production technology—tackling bottlenecks

et al. 2019

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Identification of molecular stress indicators in pikeperch Sander lucioperca correlating with rising water temperatures

Cited by 39 publications

References 75 publications

The Transcriptomic Responses of Atlantic Salmon (Salmo salar) to High Temperature Stress Alone, and in Combination with Moderate Hypoxia

The Transcriptomic Responses of Atlantic Salmon (Salmo salar) to High Temperature Stress Alone, and in Combination with Moderate Hypoxia

Characterization of Sialic Acid-Binding Immunoglobulin-Type Lectins in Fish Reveals Teleost-Specific Structures and Expression Patterns

Recent progress in European percid fish culture production technology—tackling bottlenecks

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