Identification of molecular markers in pectoral fin to predict artificial maturation of female European eels (Anguilla anguilla)

Dirks, Ron P.; Burgerhout, Erik; Brittijn, Sebastiaan A.; Wijze, Daniëlle L. de; Ozupek, Hulya; Tuinhof-Koelma, N.; Minegishi, Yuki; Jong-Raadsen, Susanne; Spaink, Herman P.; Thillart, Guido E.E.J.M. van den

doi:10.1016/j.ygcen.2014.06.023

Cited by 17 publications

(14 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As an example, we and others 1 – 3 have previously established genomic resources for the European eel ( Anguilla anguilla ), an iconic yet endangered fish species that remains resistant to efficient farming in aquaculture 4 , 5 . A draft genome 2 , several transcriptomes 1 , 3 – 10 , and reduced representation genome sequencing 11 have already shed light on its evolution and developmental biology 2 , 12 , 13 , endocrinological control of maturation 7 , 9 , metabolism 14 , disease mechanisms 10 , and population structure 15 , 16 , thereby supporting both breeding and conservation efforts. However, compared to established model organisms, funds for eel genomics are naturally limited, and consequently the quality of current genome assemblies of Anguilla species is modest at best by today’s standards (Table 1 ).…”

Section: Introductionmentioning

confidence: 99%

Rapid de novo assembly of the European eel genome from nanopore sequencing reads

Jansen

Liem

Jong-Raadsen

et al. 2017

Sci Rep

Self Cite

107

View full text Add to dashboard Cite

We have sequenced the genome of the endangered European eel using the MinION by Oxford Nanopore, and assembled these data using a novel algorithm specifically designed for large eukaryotic genomes. For this 860 Mbp genome, the entire computational process takes two days on a single CPU. The resulting genome assembly significantly improves on a previous draft based on short reads only, both in terms of contiguity (N50 1.2 Mbp) and structural quality. This combination of affordable nanopore sequencing and light weight assembly promises to make high-quality genomic resources accessible for many non-model plants and animals.

show abstract

Section: Introductionmentioning

confidence: 99%

Rapid de novo assembly of the European eel genome from nanopore sequencing reads

Jansen

Liem

Jong-Raadsen

et al. 2017

Sci Rep

Self Cite

107

View full text Add to dashboard Cite

show abstract

“…All RNAseq libraries (150–750 bp inserts) were sequenced on an Illumina HiSeq2500 sequencer as 2 × 50 nucleotides paired-end reads according to the manufacturer’s protocol. Image analysis and base calling were done using the Illumina pipeline [ 43 , 44 ]. The data discussed in this publication have been deposited in NCBI's Gene Expression Omnibus and are accessible through GEO Series accession number GSE102221 ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE102221 ).…”

Section: Methodsmentioning

confidence: 99%

Anguillicola crassus infection affects mRNA expression levels in gas gland tissue of European yellow and silver eel

2017

Self Cite

View full text Add to dashboard Cite

Using Illumina sequencing, we investigated transcriptional changes caused by the nematode Anguillicola crassus within yellow and silver eels by comparing swimbladder samples of uninfected yellow with infected yellow eels, and uninfected silver with infected silver eels, respectively. In yellow eel gas gland, the infection caused a modification of steady state mRNA levels of 1675 genes, most of them being upregulated. Functional annotation analysis based on GO terms was used to categorize identified genes with regard to swimbladder metabolism or response to the infection. In yellow eels, the most prominent category was ‘immune response’, including various inflammatory components, complement proteins, and immunoglobulins. The elevated expression of several glucose and monocarboxylate transporters indicated an attempt to maintain the level of glucose metabolism, even in due to the infection thickened swimbladder tissue. In silver eel swimbladder tissue, on the contrary, the mRNA levels of only 291 genes were affected. Genes in the categories ‘glucose metabolism’ and ‘ROS metabolism’ barely responded to the infection and even the reaction of the immune system was much less pronounced compared to infected yellow eels. However, in the category ‘extracellular matrix’, the mRNA levels of several mucin genes were strongly elevated, suggesting increased mucus production as a defense reaction against the parasite. The present study revealed a strong reaction to an Anguillicola crassus infection on mRNA expression levels in swimbladder tissue of yellow eels, whereas in silver eels the changes ware almost negligible. A possible explanation for this difference is that the silvering process requires so much energy that there is not much scope to cope with the additional challenge of a nematode infection. Another possible explanation could be that gas-secreting activity of the silver eel swimbladder was largely reduced, which could coincide with a reduced responsiveness to other challenges, like a nematode infection.

show abstract

“…11 The mucopolysaccharides in the mucus has a high value for immunoprotection and defense mechanism. 12,13 It has been proven that mucin has potential of antimicrobial and noxious properties, 14 as it plays an important role to protect the eel from pathogens. 15 Overall mucus layer on the surface of eel functions as a physical and biochemical barrier between eel and its environment.…”

Section: Biological Activities Of Asian Swamp Eel Skin Mucusmentioning

confidence: 99%

Review about the importance of skin mucus from asian swamp eel (Monopterus albus)

Hilles¹,

Mahmood²,

Kaderi³

et al. 2018

MOJPB

View full text Add to dashboard Cite

Identification of molecular markers in pectoral fin to predict artificial maturation of female European eels (Anguilla anguilla)

Cited by 17 publications

References 42 publications

Rapid de novo assembly of the European eel genome from nanopore sequencing reads

Rapid de novo assembly of the European eel genome from nanopore sequencing reads

Anguillicola crassus infection affects mRNA expression levels in gas gland tissue of European yellow and silver eel

Review about the importance of skin mucus from asian swamp eel (Monopterus albus)

Contact Info

Product

Resources

About