Identification of modifiers of the plant height in wheat using an induced dwarf mutant controlled by RhtB4c allele

Abstract

In wheat, 25 Rht genes for dwarfness are known, which include both, GA-insensitive and GA-responsive genes. The GA-insensitive Rht genes have been widely used, although, their suitability under abiotic stress conditions has been questioned. This necessitated a search for alternative GA-responsive, spontaneous and induced dwarfing genes. We earlier reported an induced dwarf mutant ('dwarf mutant-3 0 ; 44 cm), and the mutant allele was named Rht4c allele (2BL). This dwarf mutant was not suitable for cultivation due to its extra dwarf nature. Therefore, we searched for naturally occurring QTLs, which would modify the phenotype of 'dwarf-mutant-3 0 using 'mutant-assisted gene identification and characterization' (MAGIC) approach. For this purpose, the 'dwarf mutant-3 0 was crossed with a tall wheat cv. NP114 and homozygous mutant F 2 plants (* 25% of the progeny) were selected, which were phenotyped for plant height and genotyped using SSR markers. The data were utilized for QTL analysis and plant height. Six modifier QTLs were identified on chromosomes 2A, 2B and 4A. Two QTLs each on 2A and 2B were responsible for increase in plant height (described as 'enhancer modifiers'), whereas the remaining two QTLs located on 4A were responsible for reducing the plant height (described as 'suppressor modifiers'). It was hypothesized that the enhancer QTLs could be exploited for the development of semi-dwarf high yielding genotypes containing Rht4c allele. This is the first study of its kind in wheat demontsrating that the MAGIC approach could be used for identification of modifiers of the mutant phenotypes of other traits for wheat improvement.

Utilization of a Wheat50K SNP Microarray-Derived High-Density Genetic Map for QTL Mapping of Plant Height and Grain Traits in Wheat

“…The application of Rht1 (RHT-B1b) and Rht2 (RHT-D1b) in the 1960s set off a green revolution in wheat breeding. So far, 25 Rht genes have been identified in wheat [48,49]. Amongst these 25 genes, Rht1 and Rht2 are dwarfing genes that show insensitivity to gibberellins located on chromosomes 4BS and 4DS, respectively [13].…”

Overexpression of 9-cis-Epoxycarotenoid Dioxygenase Gene, IbNCED1, Negatively Regulates Plant Height in Transgenic Sweet Potato

“…The Green Revolution has promoted a significant yield increase through the development of semi-dwarf plant architecture in rice, wheat, maize, and soybean [ 21 , 44 , 45 , 46 ]. The ideal architecture for sweet potato also could promote the mechanization degree and yield of storage roots.…”

Overexpression of 9-<em>cis</em>-Epoxycarotenoid Dioxygenase Gene, <em>IbNCED1</em>, Negatively Regulates Plant Height in Transgenic Sweet Potato

“…The Green Revolution has promoted a significant yield increase through the development of semi-dwarf plant architecture in rice, wheat, maize and soybean [44][45][46][47]. The ideal architecture for sweet potato also could promote the mechanization degree and yield of storage root.…”