Identification of miRNA-mRNA Regulatory Network and Construction of Prognostic Signature in Cervical Cancer

Mei, Yong; Jiang, Pinping; Shen, Ningmei; Fu, Shilong; Zhang, Jinsong

doi:10.1089/dna.2020.5452

Cited by 11 publications

(8 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A number of studies have shown that high infiltration of M2 macrophages indicates poor prognosis [ 1 , 37 – 39 ]. In Cervical Cancer, FABP4 was considered to be correlated to immune cell infiltration [ 40 ]. To explore factors related to patient prognosis that is regulated by hub genes expression, association between hub genes expression and immune cell infiltration was analyzed.…”

Section: Discussionmentioning

confidence: 99%

Prognostic Value and Immune Infiltrates of ABCA8 and FABP4 in Stomach Adenocarcinoma

Guo

Wang

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

Background. Stomach adenocarcinoma (STAD) is a common malignancy worldwide with poor prognosis. Therefore, it is important to identify a valuable prognostic biomarker for STAD. The aim of present study was to identify novel prognostic biomarkers for STAD and evaluate the potential role of hub genes in STAD. Methods. Gene Expression Profiling Interactive Analysis (GEPIA) and Cancer RNA-Seq Nexus were performed to identify differentially expressed genes (DEGs). Subsequently, hub genes were selected by a Venn diagram, and the expression of key genes was confirmed by UALCAN database. Furthermore, survival analysis of these hub genes was performed using Oncolnc and Human Protein Atlas (HPA) database. Gene alteration status of hub genes was assessed by cBioPortal. Finally, we investigated the association between hub genes and immune cell infiltration in STAD through the Tumor Immune Estimation Resource (TIMER) and GEPIA database. Results. Three common hub genes were obtained, including 2 downregulated DEGs (ABCA8 and FABP4) and one upregulated DEG (SLC52A3). Furthermore, increased expression of ABCA8 and FABP4 and decreased expression of SLC52A3 were correlated with poor prognosis. Meanwhile, three hub genes showed genetic alterations in various datasets of STAD. Finally, our results showed that ABCA8 and FABP4 displayed a positive correlation with immune infiltration, especially in M2 macrophages. Conclusions. The results of this study suggest that ABCA8 and FABP4 may be used as prognostic biomarkers and correlated with immune infiltration in STAD.

show abstract

Section: Discussionmentioning

confidence: 99%

Prognostic Value and Immune Infiltrates of ABCA8 and FABP4 in Stomach Adenocarcinoma

Guo

Wang

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…CDK1 and TOP2A might play a critical role in controlling the genetic network related to cervical cancer incidence, progression, and metastasis [ 17 , 26 , 27 ]. Among them, some authors suggested candidate drugs for the treatment against CC [ 16 , 28 ]. Nevertheless, none of them have investigated the resistance of their suggested drugs against the independent receptors proposed by others.…”

Section: Introductionmentioning

confidence: 99%

Bioinformatics Screening of Potential Biomarkers from mRNA Expression Profiles to Discover Drug Targets and Agents for Cervical Cancer

Reza

Harun-Or-Roshid

Islam

et al. 2022

IJMS

View full text Add to dashboard Cite

Bioinformatics analysis has been playing a vital role in identifying potential genomic biomarkers more accurately from an enormous number of candidates by reducing time and cost compared to the wet-lab-based experimental procedures for disease diagnosis, prognosis, and therapies. Cervical cancer (CC) is one of the most malignant diseases seen in women worldwide. This study aimed at identifying potential key genes (KGs), highlighting their functions, signaling pathways, and candidate drugs for CC diagnosis and targeting therapies. Four publicly available microarray datasets of CC were analyzed for identifying differentially expressed genes (DEGs) by the LIMMA approach through GEO2R online tool. We identified 116 common DEGs (cDEGs) that were utilized to identify seven KGs (AURKA, BRCA1, CCNB1, CDK1, MCM2, NCAPG2, and TOP2A) by the protein–protein interaction (PPI) network analysis. The GO functional and KEGG pathway enrichment analyses of KGs revealed some important functions and signaling pathways that were significantly associated with CC infections. The interaction network analysis identified four TFs proteins and two miRNAs as the key transcriptional and post-transcriptional regulators of KGs. Considering seven KGs-based proteins, four key TFs proteins, and already published top-ranked seven KGs-based proteins (where five KGs were common with our proposed seven KGs) as drug target receptors, we performed their docking analysis with the 80 meta-drug agents that were already published by different reputed journals as CC drugs. We found Paclitaxel, Vinorelbine, Vincristine, Docetaxel, Everolimus, Temsirolimus, and Cabazitaxel as the top-ranked seven candidate drugs. Finally, we investigated the binding stability of the top-ranked three drugs (Paclitaxel, Vincristine, Vinorelbine) by using 100 ns MD-based MM-PBSA simulations with the three top-ranked proposed receptors (AURKA, CDK1, TOP2A) and observed their stable performance. Therefore, the proposed drugs might play a vital role in the treatment against CC.

show abstract

“…Previous studies have reported atypical expression levels of various miRNAs, including miR-101–3p. Reportedly, miR-101–3p demonstrates reduced expression in several cancer types such as ovarian cancer [ 20 ], cervical cancer [ 36 ] and hepatocellular carcinoma [ 29 ]. Furthermore, its therapeutic effects and molecular biological mechanisms in these tumour types have been well elucidated.…”

Section: Discussionmentioning

confidence: 99%

Nanomedicine promotes ferroptosis to inhibit tumour proliferation in vivo

Luo

Niu

et al. 2021

Redox Biology

View full text Add to dashboard Cite

miR-101–3p may play a therapeutic role in various tumours. However, its anti-tumour mechanism remains unclear, and a definitive strategy to treat tumour cells in vivo is lacking. The objective of this study was to investigate the inhibitory mechanism of miR-101–3p on tumour cells and to develop relevant nanomedicines for in vivo therapy. The expression levels of miR-101–3p and its target protein TBLR1 in tumour tissues and cells were detected, and their relationship with ferroptosis was clarified. Furthermore, the efficacy of nanocarriers in achieving in vivo therapeutic gene delivery was evaluated. Nanomedicine was further developed, with the anti-proliferative in vivo therapeutic effect validated using a subcutaneous xenograft cancer model. The expression level of miR-101–3p negatively correlated with clinical tumour size and TNM stage. miR-101–3p restores ferroptosis in tumour cells by directly targeting TBLR1, which in turn promotes apoptosis and inhibits proliferation. We developed nanomedicine that can deliver miR-101–3p to tumour cells in vivo to achieve ferroptosis recovery, as well as to inhibit in vivo tumour proliferation. The miR-101–3p/TBLR1 axis plays an important role in tumour ferroptosis. Nanopharmaceuticals that increase miR-101–3p levels may be effective therapies to inhibit tumour proliferation.

show abstract

Identification of miRNA-mRNA Regulatory Network and Construction of Prognostic Signature in Cervical Cancer

Cited by 11 publications

References 48 publications

Prognostic Value and Immune Infiltrates of ABCA8 and FABP4 in Stomach Adenocarcinoma

Prognostic Value and Immune Infiltrates of ABCA8 and FABP4 in Stomach Adenocarcinoma

Bioinformatics Screening of Potential Biomarkers from mRNA Expression Profiles to Discover Drug Targets and Agents for Cervical Cancer

Nanomedicine promotes ferroptosis to inhibit tumour proliferation in vivo

Contact Info

Product

Resources

About