Identification of miRNA-mRNA crosstalk in laryngeal squamous cell carcinoma

Fei, Yonghua; Guo, Ping; Wang, Fuling; Hu, Li; Yan, Lei; Li, Wei; Xun, Xuehong; Lu, Feng-xiang

doi:10.3892/mmr.2017.7123

Cited by 8 publications

(5 citation statements)

References 44 publications

(55 reference statements)

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“…Initiation and progression of cancer are often caused by an imbalance between oncogenes and tumor suppressive genes. From The Cancer Genome Atlas (TCGA) database, a number of differentially expressed miRNAs in LSCC have been identified [11]. Several miRNAs, including miR-26a and miR-153, emerged as powerful regulators in LSCC, as indicated in recent studies [12,13].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-625 inhibits cell invasion and epithelial–mesenchymal transition by targeting SOX4 in laryngeal squamous cell carcinoma

Tao

Dai

et al. 2019

Bioscience Reports

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Introduction: Laryngeal squamous cell carcinoma (LSCC) is a highly aggressive malignant cancer, but the molecular mechanisms underlying its development and progression remain largely elusive. The purpose of the present study is to investigate the expression profile and functional role of microRNA-625 (miR-625) in LSCC.Materials and methods: LSCC tissues and adjacent normal tissues were collected from 86 LSCC patients. The expression levels of miR-625 and SOX4 mRNA in tissues and cells were detected by RT-qPCR analysis. The expression levels of SOX4 and EMT-related proteins were detected by western blot analysis. In vitro cell proliferation, migration, and invasion were detected by MTT assay, colony formation assay, wound healing assay, and transwell invasion assay, respectively. Dual-luciferase reporter assay was performed to verify the binding relationship between miR-625 and the 3′-UTR of SOX4.Results: The results demonstrated that miR-625 is significantly down-regulated in clinical LSCC tissues, and its low expression may be closely associated with unfavorable clinicopathological characteristics of LSCC patients. Overexpression of miR-625 significantly suppressed the proliferation, migration, invasion, and EMT of LSCC cells. Furthermore, SOX4 was validated as a direct target of miR-625 in LSCC cells, and rescue experiments suggested that restoration of SOX4 blocked the tumor suppressive role of miR-625 in LSCC cells.Conclusions: Taken together, these findings highlighted a critical role of miR-625 in the pathogenesis of LSCC, and restoration of miR-625 could be considered as a potential therapeutic strategy against this fatal disease.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-625 inhibits cell invasion and epithelial–mesenchymal transition by targeting SOX4 in laryngeal squamous cell carcinoma

Tao

Dai

et al. 2019

Bioscience Reports

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“…9 d). ECM-receptor interaction was considered as potential role in participating in multiple tumor biology such as motility, invasion, and metastasis process of LSCC [ 32 ]. Researchers found that silencing of the WNT-5A receptors Frizzled 8 (FZD8) and RYK attenuated TGF-β-induced ECM expression [ 33 ]; future work needs to be done to analyze the expression status of RYK in different tumor stages and its role in progression of LSCC.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis reveals COPB2 and RYK associated with tumor stages of larynx squamous cell carcinoma

et al. 2022

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Background Laryngeal squamous cell carcinoma (LSCC) is one of the highly aggressive malignancy types of head and neck squamous cell carcinomas; genes involved in the development of LSCC still need exploration. Methods We downloaded expression profiles of 96 (85 in advanced stage and 11 in early stage) LSCC patients from TCGA-HNSC. Function enrichment and protein-protein interactions of genes in significant modules were conducted. Univariate and multivariate Cox regression analyses were performed to explore potential prognostic biomarkers for LSCC. The expression levels of genes at different stages were compared and visualized via boxplots. Immune infiltration was examined by the CIBERSORTx web-based tool and depicted with ggplot2. Gene set enrichment analysis (GSEA) was utilized to analyze functional enrichment terms and pathways. Immunohistochemical staining (IHC) was used to verify the expression of genes in the LSCC samples. Results We identified 25 modules, including 3 modules significantly related to tumor stages of LSCC via weighted gene co-expression network analysis (WGCNA). UIMC1, NPM1, and DCTN4 in the module ‘cyan’, TARS in the module ‘darkorange’, and COPB2 and RYK in the module ‘lightyellow’ showed statistically significant relation to overall survival. The expression of COPB2, DCTN4, RYK, TARS, and UIMC1 indicated association with the change of fraction of immune cells in LSCC patients; two genes, COPB2 and RYK, indicated different expression in various tumor stages of LSCC. Finally, COPB2 and RYK showed high-expression in tumor tissues of advanced LSCC patients. Conclusions Our study provided a potential perceptive in analyzing progression of LSCC cells and exploring prognostic genes.

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“…Notably, miRNAs influence the occurrence, development and metastasis of LSCC through complex mechanisms, and thus the discovery of new miRNAs in LSCC tissues is very important for the diagnosis and treatment of LSCC ( Liu & Ye, 2019 ). The purposes of this study were to screen for differentially expressed miRNAs and mRNAs in LSCC using molecular biology and bioinformatics techniques, to successfully predict miRNA target genes, and to construct a miRNA-mRNA regulatory network based on the relationships between miRNAs, mRNA, of miRNA target genes and differential expression ( Guan et al, 2015 ; Fei et al, 2017 ; Ma et al, 2021 ). Next, further evaluation was performed using gene ontology (GO) and Kyoto Encyclopedia for gene and genome (KEGG) path analysis.…”

Section: Introductionmentioning

confidence: 99%

Prediction of the mechanism of miRNAs in laryngeal squamous cell carcinoma based on the miRNA-mRNA regulatory network

Dai

et al. 2021

PeerJ

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In this study, a bioinformatics analysis is conducted to screen differentially expressed miRNAs and mRNAs in laryngeal squamous cell carcinoma (LSCC). Based on this information, we explored the possible roles of miRNAs in the pathogenesis of LSCC. The RNA-Seq data from 79 laryngeal cancer samples in the Gene Expression Omnibus (GEO) database were sorted. Differentially expressed miRNAs and mRNAs in LSCC are screened using the PERL programming language, and it was analysed by Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). The miRNA-mRNA regulatory network of LSCC is constructed using Cytoscape software. Then, quantitative real-time PCR (QRT- PCR), Cell Counting Kit-8 (CCK8) and flow cytometry analysis we are used to further validate key miRNAs. We identified 99 differentially expressed miRNAs and 2,758 differentially expressed mRNAs in LSCC tissues from the GEO database. Four more important miRNAs displaying a high degree of connectivity are selected, these results suggest that they play an important role in the pathogenesis of LSCC. As shown in the present study, we identified specific miRNA-mRNA networks associated with the occurrence and development of LSCC through bioinformatics analysis. We found a miRNA molecule closely related to LSCC based on miRNA-mRNA network: miR-140-3p was down-regulated in LSCC. In addition, the potential antitumor effect of miR-140-3p in LSCC was verified in the experiment, and it was proved that overexpression of miR-140-3p could inhibit the proliferation of LSCC cells and promote cell apoptosis, suggesting that miR-140-3p may be a potential tumor marker in LSCC.

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Identification of miRNA-mRNA crosstalk in laryngeal squamous cell carcinoma

Cited by 8 publications

References 44 publications

MicroRNA-625 inhibits cell invasion and epithelial–mesenchymal transition by targeting SOX4 in laryngeal squamous cell carcinoma

MicroRNA-625 inhibits cell invasion and epithelial–mesenchymal transition by targeting SOX4 in laryngeal squamous cell carcinoma

Comprehensive analysis reveals COPB2 and RYK associated with tumor stages of larynx squamous cell carcinoma

Prediction of the mechanism of miRNAs in laryngeal squamous cell carcinoma based on the miRNA-mRNA regulatory network

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