Identification of miR-30b as an oncogene in renal cell carcinoma

Abstract

microRNAs (miRs) have been investigated as a novel class of regulators of cellular processes, including proliferation, apoptosis and metabolism. In particular, miR‑30b has been demonstrated to be deregulated in certain types of cancer, including lung, colorectal and gastric cancer. Previous studies of miR‑30b in renal clear cell carcinoma demonstrated that the expression level of miR‑30b was associated with distant metastasis. However, the function of miR‑30b in renal cell carcinoma (RCC) remained to be elucidated. In the present study, the expression of miR‑30b in 31 paired RCC tissues from four cell lines (786‑O, 769‑P, ACHN and 293T) was detected by reverse transcription‑quantitative polymerase chain reaction. In addition, the effect of miR‑30b on cell proliferation in RCC cells was also determined using MTT and Cell Counting Kit‑8 assay analyses. Furthermore, the function of miR‑30b in cell migration and invasion was determined by wound scratch and Transwell assays. Flow cytometry was also performed to quantify the effect of miR‑30b on cell apoptosis. The results of the current study indicated that miR‑30b was upregulated in RCC tissues from affected cell lines when compared with adjacent normal tissues and a normal kidney cell line, which is different to the downregulation of miR‑30b as observed in other types of cancer. miR‑30b is associated with RCC cell proliferation, invasion, migration and apoptosis, which indicated that miR‑30b acts as an oncogene in RCC. To the best of our knowledge, the present study is the first to demonstrate the upregulation of miR‑30b in RCC tissues and describe miR‑30b as an oncogene in RCC in the regulation of cell proliferation, migration, invasion and apoptosis. Further studies will define the target gene of miR‑30b in RCC and investigate the potential role of miR‑30b as a biomarker for RCC.

Down-regulated microRNA-30b-3p inhibits proliferation, invasion and migration of glioma cells via inactivation of the AKT signaling pathway by up-regulating RECK

“…More specifically, previous findings suggest that knockdown of Proteolipid protein 2 can inhibit cell proliferation, migration and invasion of glioma with decrease of MMP-2 and MMP-9 [35]. Furthermore, elevated expression of miR-30b found in renal cell carcinoma (RCC) cells was demonstrated to promote RCC cell proliferation, invasion and migration but inhibit cell apoptosis [36]. In line with our results, accumulating evidences show that RECK can inhibit tumor angiogenesis, migration, invasion and metastasis in glioma [37–39].…”

miR‐129‐5p inhibits proliferation, migration, and invasion in rectal adenocarcinoma cells through targeting E2F7

“…Other studies also found aberrantly lower‐expression of miRNAs in colorectal tissues in contrast with normal tissues (Akao, Nakagawa, & Naoe, 2006; Y. Liu et al, 2017; Mamoori et al, 2017; Mascarello, Krous, & Carpenter, 1993). Besides identifying the abnormally expressed miRNAs in cancer cells, increasing studies currently aim at investigating the mechanisms involved for miRNAs to regulate cancer progression (Huang & Lu, 2017; Jin et al, 2017; X. Zhang, Li, Tan, Yu, & Pei, 2017). It has been widely accepted that miRNAs regulate cancer progression via targeting downstream genes to regulate their expression or translation, and one of the mechanisms involved is through targeting the transcription factors to regulate cell progressions (Cao, Yu, Feng, Chen, & Liang, 2017; W. Li, Liang, et al, 2017; Y. Li, Su, Li, Chen, & Zhang, 2017; Qiu & Dou, 2017).…”

“…Other studies also found aberrantly lower-expression of miRNAs in colorectal tissues in contrast with normal tissues (Akao, Nakagawa, & Naoe, 2006;Mamoori et al, 2017;Mascarello, Krous, & Carpenter, 1993). Besides identifying the abnormally expressed miRNAs in cancer cells, increasing studies currently aim at investigating the mechanisms involved for miRNAs to regulate cancer progression (Huang & Lu, 2017;Jin et al, 2017;X. Zhang, Li, Tan, Yu, & Pei, 2017).…”

miR‐30b‐5p up‐regulation related to the dismal prognosis for patients with renal cell cancer

“…The above result suggests that miR‐30 may play a vital regulatory part during human cancer development. As discovered in our prior work, the expression of miR‐30b increases within RCC tissue samples, which may act as the oncogenic gene in RCC to regulate the proliferation of cells 13 . However, it remains unclear about whether miR‐30b‐5p can be used to be the candidate biomarker for predicting RCC prognosis.…”