Identification of miR-2400 gene as a novel regulator in skeletal muscle satellite cells proliferation by targeting MYOG gene

Zhang, Wei Wei; Tong, Hui; Sun, Xiao Feng; Hu, Qian; Yu, Yang; Li, Shunfeng; Yan, Yun; Li, Guang Peng

doi:10.1016/j.bbrc.2015.05.112

Cited by 21 publications

(17 citation statements)

References 33 publications

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“…It was reported that miR-2400 is a novel and speci c bovine miRNA because it is not detectable in other mammalian genomes [33]. It was reported that in skeletal muscle-derived satellite cells (MDSCs), the overexpression of miR-2400 promotes proliferation by targeting the MYOG gene [34]. In this study, we also nd the same function of miR-2400 in bovine primary myoblast proliferation.…”

Section: Discussionsupporting

confidence: 54%

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Circular RNA circMYL1 inhibit proliferation and promote differentiation of myoblasts by sponging miR-2400

Elnour

Wang

Zhansaya

et al. 2020

Preprint

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Background Circular RNAs (circRNAs) are a class of endogenous non-coding RNAs (ncRNAs) involved in regulating skeletal muscle development by sponging microRNAs (miRNAs). This study aimed to define the circMYL1 molecular mechanisms in the regulation of bovine myogenesis and to disclose its regulatory mechanism through miR-2400 interaction. Methods The potential role of circMYL1 was identified in the proliferation of bovine myoblast through mRNA and protein expression of proliferation marker genes (PCNA, CyclinD1, and CDK2), cell counting kit-8 (CCK8) assay, flow cytometry analysis, and EdU assay. Analysis of the expression of differentiation marker genes (MyoD, MyoG, and MYH2) and immunofluorescence of Myosin heavy chain (MyHC) was used to assess cell differentiation. Results The proliferation analysis revealed that circMYL1 inhibited the proliferation of bovine primary myoblast. Furthermore, the differentiation analysis demonstrated that circMYL1 promoted the differentiation of bovine primary myoblast. The luciferase screening and RNA immunoprecipitation (RIP) assays found that circMYL1 could have interaction with miR-2400. Additionally, we demonstrated that miR-2400 promoted proliferation and inhibited differentiation of bovine primary myoblast, while circMYL1 may eliminate the effects of miR-2400, as showed by rescue experiments. Conclusions Our results revealed that a novel circular RNA of circMYL1 could inhibit proliferation and promote differentiation of myoblast by sponging miR-2400.

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Section: Discussionsupporting

confidence: 54%

“…It is, therefore, a speci c bovine miRNA [33]. Recently, it was reported that the overexpression of miR-2400 promotes the proliferation of skeletal muscle-derived satellite cells (MDSCs) by targeting the MYOG gene [34]. However, the function of miR-2400 in the regulation of myoblast differentiation was not reported.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circMYL1 inhibit proliferation and promote differentiation of myoblasts by sponging miR-2400

Elnour

Wang

Zhansaya

et al. 2020

Preprint

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“…Taxis found that miR-339a is associated with cattle challenged with bovine viral diarrhea virus 49 . Studies have found that miR-2400 promotes bovine preadipocyte proliferation 50 and regulates the proliferation of skeletal muscle satellite cells by targeting the MYOG gene 51 . miR-30c has been shown to regulate cell proliferation and differentiation as well as to promote Schwann cell remyelination following peripheral nerve injury 52 .…”

Section: Discussionmentioning

confidence: 99%

“…Host circRNA genes were used to list the gene names that were submitted to DAVID software for GO analysis 50 . KEGG enrichment analysis of the host circRNA genes was performed with KOBAS software 51 . Scores with P < 0.05 were considered significant for enrichment analysis.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide analysis of circular RNAs in bovine cumulus cells treated with BMP15 and GDF9

Jiang

et al. 2018

Sci Rep

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Circular RNAs (circRNAs) are important members of the non-coding RNA family, and those relating to animal physiologies have been widely studied in recent years. This study aimed to explore the roles of circRNAs in the regulation of follicular development. We constructed four bovine cumulus cell cDNA libraries, including a negative control group (NC) and groups treated with BMP15, GDF9 and BMP15 + GDF9, and we sequenced the libraries on the Illumina HiSeq Xten platform. We identified 1706 circRNAs and screened for differential circRNA expression. We conducted a bioinformatics analysis of these circRNAs and screened for differential circRNAs. Functional annotation and enrichment analysis of the host genes showed that the differential circRNAs were related to locomotion, reproduction, biological adhesion, growth, rhythmic processes, biological phases and hormone secretion. According to the differential expression of circRNA between groups, there were 3 up-regulated and 6 down-regulated circRNAs in the BMP15 group as well as 12 up-regulated and 24 down-regulated circRNAs in the GDF9 group. Co-addition of both BMP15 and GDF9 resulted in 15 up-regulated and 13 down-regulated circRNAs. circ_n/a_75,circ_12691_1 and circ_n/a_303 were altered in both the BMP15 and GDF9 groups as well as in the BMP15 + GDF9 combination group. We focused on these three circRNAs because they were potentially associated with the additive effect of BMP15 and GDF9. Quantitative PCR analysis showed that the expression levels of these three circRNAs were consistent with the sequencing results. In addition, the target miRNAs of circ_n/a_75 and circ_n/a_303, miR-339a, miR-2400 and miR-30c, were down-regulated in the experimental group, which was in contrast to the circRNAs trend. These findings demonstrated that BMP15 and GDF9 may regulate the target gene through circRNA, as a miRNA sponge, in order to regulate the status of bovine cumulus cells and affect follicular development.

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“…MiR-101a was a positive regulator of goat skeletal muscle satellite cells differentiation 7 . MiR-2400 promotes bovine skeletal muscle satellite cell proliferation by targeting MYOG 8 .…”

Section: Introductionmentioning

confidence: 99%

MiR-2425-5p targets RAD9A and MYOG to regulate the proliferation and differentiation of bovine skeletal muscle-derived satellite cells

Tong

Jiang

Zhang

et al. 2017

Sci Rep

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Our group previously identified miR-2425-5p, a unique bovine miRNA; however, its biological function and regulation in muscle-derived satellite cells (MDSCs) remain unclear. Herein, stem-loop RT-PCR results showed that miR-2425-5p increased during MDSCs proliferation, but decreased during differentiation. Cell proliferation was examined using EdU assays, cyclin B1 (CCNB1) and proliferating cell nuclear antigen (PCNA) western blot (WB) and flow cytometry analysis. These results showed that miR-2425-5p mimics (miR-2425-M) enhanced MDSCs proliferation, whereas, miR-2425-5p inhibitor (miR-2425-I) had opposite effect. Conversely, cell differentiation studies by desmin (DES) immunofluorescence, myotubes formation, and myosin heavy chain 3 (MYH3) WB analyses revealed that miR-2425-M and miR-2425-I blocked and promoted MDSCs differentiation, respectively. Moreover, luciferase reporter, RT-PCR, and WB assays showed that miR-2425-5p directly targeted the 3′-UTR of RAD9 homolog A (RAD9A) and myogenin (MYOG) to regulate their expression. Rescue experiment showed RAD9A inhibited the proliferation of MDSCs through miR-2425-5p. In addition, we found that miR-2425-5p expression was regulated by its host gene NCK associated protein 5-like (NCKAP5L) rather than being transcribed independently as a separate small RNA. Collectively, these data indicate that miR-2425-5p is a novel regulator of bovine MDSCs proliferation and differentiation and provides further insight into the biological functions of miRNA in this species.

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Identification of miR-2400 gene as a novel regulator in skeletal muscle satellite cells proliferation by targeting MYOG gene

Cited by 21 publications

References 33 publications

Circular RNA circMYL1 inhibit proliferation and promote differentiation of myoblasts by sponging miR-2400

Circular RNA circMYL1 inhibit proliferation and promote differentiation of myoblasts by sponging miR-2400

Genome-wide analysis of circular RNAs in bovine cumulus cells treated with BMP15 and GDF9

MiR-2425-5p targets RAD9A and MYOG to regulate the proliferation and differentiation of bovine skeletal muscle-derived satellite cells

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