Identification of minor lignans, alkaloids, and phenylpropanoid glycosides in Magnolia officinalis by HPLC‒DAD‒QTOF-MS/MS

Guo, Keke; Tong, Chaoying; Fu, Qiachi; Xu, Jinju; Shi, Shuyun; Xiao, Yecheng

doi:10.1016/j.jpba.2019.03.044

Cited by 44 publications

(23 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, the barks are also widely applied in cosmetics as an important ingredient ( Li et al., 2007 ), and its flowers have been approved as a raw material in nutritional supplement. The main components of M. officinalis include lignans, alkaloids, volatile oils, and other constituents, of which magnolol, as well as honokiol are the two main active compounds ( Guo et al., 2019 ; Luo et al., 2019 ). Given its multicomponent nature, numerous pharmacological activities of M. officinalis have been reported, such as anti-tumor, anti-inflammation, anti-virus, anti-microorganism, and so on ( Yang et al., 2016 ).…”

Section: Introductionmentioning

confidence: 99%

The chromosome-scale genome of Magnolia officinalis provides insight into the evolutionary position of magnoliids

et al. 2021

View full text Add to dashboard Cite

Summary Magnolia officinalis , a representative tall aromatic tree of the Magnoliaceae family, is a medicinal plant that is widely used in diverse industries from medicine to cosmetics. We report a chromosome-scale draft genome of M. officinalis , in which ∼99.66% of the sequences were anchored onto 19 chromosomes with the scaffold N50 of 76.62 Mb. We found that a high proportion of repetitive sequences was a common feature of three Magnoliaceae with known genomic data. Magnoliids were a sister clade to eudicots-monocots, which provided more support for understanding the phylogenetic position among angiosperms. An ancient duplication event occurred in the genome of M. officinalis and was shared with Lauraceae. Based on RNA-seq analysis, we identified several key enzyme-coding gene families associated with the biosynthesis of lignans in the genome. The construction of the M. officinalis genome sequence will serve as a reference for further studies of Magnolia, as well as other Magnoliaceae.

show abstract

Section: Introductionmentioning

confidence: 99%

The chromosome-scale genome of Magnolia officinalis provides insight into the evolutionary position of magnoliids

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Compounds with a matching degree of more than 80 points and delta mass range less than ±5 ppm were screened. Referring to the relevant literature of Magnolia officinalis, 30 provisional compounds were screened; the results are shown in Table 1 [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

“…Magnolia officinalis has a long medicinal history, and its research is also deepening [ 30 ]. Literature search showed that Magnolia officinalis mainly contains phenols, alkaloids, volatile oils, and other components [ 23 ]. In traditional Chinese medicine, Magnolia officinalis is often used to treat gastrointestinal diseases.…”

Section: Discussionmentioning

confidence: 99%

Gastroprotective Effect of Ethanol Extracts from Bark of Magnolia officinalis on Ethanol-Induced Gastric Mucosal Damage in Rats

Wang

Zhang

et al. 2021

BioMed Research International

View full text Add to dashboard Cite

Background. Magnolia officinalis Rehd. and Wils. is widely used in Asian countries because of its multiple pharmacological effects. This study investigated the gastroprotective effect and mechanisms of the ethanol extracts from the bark of Magnolia officinalis (MOE) against ethanol-induced gastric mucosal damage in rats. Methods. MOE was prepared by reflux extraction with 70% ethanol, and its main compounds were analyzed by UPLC-Q-Exactive Orbitrap-MS. DPPH, ABTS, and FRAP methods were used to evaluate the antioxidant capacity of MOE in vitro. The gastroprotective effects of MOE were evaluated by the area of gastric injury, H&E (hematoxylin-eosin), and PAS (periodic acid-Schiff). The mechanism was explored by measuring the levels of cytokines and protein in the NF-κB signaling pathway. Results. 30 compounds were identified from MOE, mainly including lignans and alkaloids. MOE presented a high antioxidant activity in several oxidant in vitro systems. Gastric ulcer index and histological examination showed that MOE reduced ethanol-induced gastric mucosal injury in a dose-dependent manner. MOE pretreatment significantly restored the depleted activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) enzymes, reduced malondialdehyde (MDA), and prostaglandin E2 (PGE2) levels in the gastric tissue in rats. In addition, MOE also inhibited the activation of nuclear factor kappa B (NF-κB) pathway and decreased the production of proinflammatory cytokines. Conclusions. The gastroprotective effect of MOE was attributed to the inhibition of oxidative stress and the NF-κB inflammatory pathway. The results provided substantial evidence that MOE could be a promising phytomedicine for gastric ulcer prevention.

show abstract

“…The advantages of using HSCCC to isolate compounds from a complex mixture include high recovery, low consumption of solvents, and the non-denaturing and reversible adsorption of target compounds [14]. Consequently, HSCCC has been frequently employed as an efficient separation and preparative tool to isolate natural compounds from complicated mixtures of components.…”

Section: Resultsmentioning

confidence: 99%

Phytochemical Profile and Antidepressant Effect of Ormosia henryi Prain Leaf Ethanol Extract

Lü

Zhu

et al. 2019

IJMS

View full text Add to dashboard Cite

The Ormosia henryi Prain leaf (OHPL) is a new bioactive resource with potential antidepressant activity, but few reports have confirmed its chemical composition or antidepressant effect. To investigate the phytochemical profile of OHPL ethanol extract (OHPLE), six flavone C-glycosides and two flavone O-glycosides were purified by high-speed counter-current chromatography combined with preparative high-performance liquid chromatography (HSCCC-prep-HPLC). The eight isolated compounds were identified by NMR and MS. Forty-six flavonoids, including flavones, flavone C-glycosides, flavone O-glycosides, isoflavones, isoflavone O-glycosides, prenylflavones and polymethoxyflavones were definitively or tentatively identified from OHPLE using ultra-performance liquid chromatography/ electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS/MS) on the basis of fragment ions that are characteristic of these isolated compounds. The results of the antidepressant assay suggest that OHPLE significantly improved depression-related behaviors of chronic unpredictable mild stress (CUMS) mice. The observed changes in these mice after OHPLE treatment were an increased sucrose preference index, reduced feeding latency, prolonged tail suspension time, and upregulated expression of brain-derived neurotrophic factor (BDNF). The details of the phytochemicals and the antidepressant effect of OHPLE are reported here for the first time. This study indicates that the OHPL, enriched in flavone C-glycosides, is a new resource that might be potentially applied in the field of nutraceuticals (or functional additives) with depression-regulating functions.

show abstract

Identification of minor lignans, alkaloids, and phenylpropanoid glycosides in Magnolia officinalis by HPLC‒DAD‒QTOF-MS/MS

Cited by 44 publications

References 30 publications

The chromosome-scale genome of Magnolia officinalis provides insight into the evolutionary position of magnoliids

The chromosome-scale genome of Magnolia officinalis provides insight into the evolutionary position of magnoliids

Gastroprotective Effect of Ethanol Extracts from Bark of Magnolia officinalis on Ethanol-Induced Gastric Mucosal Damage in Rats

Phytochemical Profile and Antidepressant Effect of Ormosia henryi Prain Leaf Ethanol Extract

Contact Info

Product

Resources

About