Identification of microsatellite markers for a worldwide distributed, highly invasive ant species Tapinoma melanocephalum (Hymenoptera: Formicidae)

Zima, Jan; Lebrasseur, Ophélie; Borovanská, Michaela; Janda, Milan

doi:10.14411/eje.2016.053

Cited by 7 publications

(6 citation statements)

References 22 publications

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“…The homozygous excess caused the markers to show signs of null alleles and depart from the HWE. This study revealed a similar result to that of Zima et al 2016 , who detected a high level of homozygosity for the Tapinoma melanocephalum microsatellite markers. The excess of homozygosity may arise from biological processes, such as selection, the founder effect, bottleneck, or inbreeding ( Goodisman and Hahn 2005 ).…”

Section: Resultssupporting

confidence: 87%

Development and Characterization of Novel Polymorphic Microsatellite Markers for Tapinoma indicum (Hymenoptera: Formicidae)

Lim

Majid

2021

Journal of Insect Science

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Tapinoma indicum (Forel) (Hymenoptera: Formicidae) is a nuisance pest in Asia countries. However, studies on T. indicum are limited, especially in the field of molecular biology, to investigate the species characteristic at the molecular level. This paper aims to provide valuable genetic markers as tools with which to study the T. indicum population. In this study, a total of 143,998 microsatellite markers were developed based on the 2.61 × 106 microsatellites isolated from T. indicum genomic DNA sequences. Fifty selected microsatellite markers were amplified with varying numbers of alleles ranging from 0 to 19. Seven out of fifty microsatellite markers were characterized for polymorphism with the Hardy–Weinberg equilibrium (HWE) and linkage disequilibrium (LD) analysis. All seven microsatellite markers demonstrated a high polymorphic information content (PIC) value ranging from 0.87 to 0.93, with a mean value of 0.90. There is no evidence of scoring errors caused by stutter peaks, no large allele dropout, and no linkage disequilibrium among the seven loci; although loci Ti-Tr04, Ti-Tr09, Ti-Te04, Ti-Te13, and Ti-Pe5 showed signs of null alleles and deviation from the HWE due to excessive homozygosity. In conclusion, a significant amount of microsatellite markers was developed from the data set of next-generation sequencing, and seven of microsatellite markers were validated as informative genetic markers that can be utilized to study the T. indicum population.

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Section: Resultssupporting

confidence: 87%

Development and Characterization of Novel Polymorphic Microsatellite Markers for Tapinoma indicum (Hymenoptera: Formicidae)

Lim

Majid

2021

Journal of Insect Science

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“…Species‐specific microsatellite primers do not exist for T . sessile ; instead, we tested 39 markers shown to amplify in closely related species (Berman et al, 2014; Butler et al, 2014; Krieger & Keller, 1999; Zheng et al, 2018; Zima et al, 2016). Forward primers were affixed with an M13 tail to enable PCR multiplexing via fluorescent labelling with 6‐FAM, VIC, PET, and NED (Boutin‐Ganache et al, 2001).…”

Section: Methodsmentioning

confidence: 99%

Consistent signatures of urban adaptation in a native, urban invader ant Tapinoma sessile

et al. 2021

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Biological invasions are becoming more prevalent due to the rise of global trade and expansion of urban areas. Ants are among the most prolific invaders with many exhibiting a multiqueen colony structure, dependent colony foundation and reduced internest aggression. Although these characteristics are generally associated with the invasions of exotic ants, they may also facilitate the spread of native ants into novel habitats. Native to diverse habitats across North America, the odorous house ant Tapinoma sessile has become abundant in urban environments throughout the United States. Natural colonies typically have a small workforce, inhabit a single nest, and are headed by a single queen, whereas urban colonies tend to be several orders of magnitude larger, inhabit multiple nests (i.e., polydomy) and are headed by multiple queens (i.e., polygyny). Here, we explore and compare the population genetic and breeding structure of T. sessile within and between urban and natural environments in several localities across its distribution range. We found the social structure of a colony to be a plastic trait in both habitats, although extreme polygyny was confined to urban habitats. Additionally, polydomous colonies were only present in urban habitats, suggesting T. sessile can only achieve supercoloniality within urbanized areas. Finally, we identified strong differentiation between urban and natural populations in each locality and continent‐wide, indicating cities may restrict gene flow and exert intense selection pressure. Overall, our study highlights urbanization's influence in charting the evolutionary course for species.

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“…We assigned colonies to species based on worker morphology (Seifert et al, 2017). We confirmed identifications by typing three workers per colony at 11 diagnostic microsatellite markers: Ant1368, Ant2794, Ant3648, Ant4155, Ant5035, Ant7249, Ant8424 and Ant9218 (Butler et al, 2014), and TM_3, TM_10 and TM_16 (Zima et al, 2016). As reference samples, we used workers from all four species in the T. nigerrimum complex (T. darioi, T. ibericum, T. nigerrium and T. magnum) that were previously identified by B. Seifert.…”

Section: Acclimation Experimentsmentioning

confidence: 96%

Increased acclimation ability accompanies a thermal niche shift of a recent invasion

Bujan

Charavel

Bates

et al. 2020

Journal of Animal Ecology

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Globalization is removing dispersal barriers for the establishment of invasive species and enabling their spread to novel climates. New thermal environments in the invaded range will be particularly challenging for ectotherms, as their metabolism directly depends on environmental temperature. However, we know little about the role climatic niche shifts play in the invasion process, and the underlining physiological mechanisms. We tested if a thermal niche shift accompanies an invasion, and if native and introduced populations differ in their ability to acclimate thermal limits. We used an alien ant species—Tapinoma magnum—which recently started to spread across Europe. Using occurrence data and accompanying climatic variables, we measured the amount of overlap between thermal niches in the native and invaded range. We then experimentally tested the acclimation ability in native and introduced populations by incubating T. magnum at 18, 25 and 30°C. We measured upper and lower critical thermal limits after 7 and 21 days. We found that T. magnum occupies a distinct thermal niche in its introduced range, which is on average 3.5°C colder than its native range. Critical thermal minimum did not differ between populations from the two ranges when colonies were maintained at 25 or 30°C, but did differ after colony acclimation at a lower temperature. We found twofold greater acclimation ability of introduced populations to lower temperatures, after prolonged incubation at 18°C. Increased acclimation ability of lower thermal limits could explain the expansion of the realized thermal niche in the invaded range, and likely contributed to the spread of this species to cooler climates. Such thermal plasticity could be an important, yet so far understudied, factor underlying the expansion of invasive insects into novel climates.

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Identification of microsatellite markers for a worldwide distributed, highly invasive ant species Tapinoma melanocephalum (Hymenoptera: Formicidae)

Cited by 7 publications

References 22 publications

Development and Characterization of Novel Polymorphic Microsatellite Markers for Tapinoma indicum (Hymenoptera: Formicidae)

Development and Characterization of Novel Polymorphic Microsatellite Markers for Tapinoma indicum (Hymenoptera: Formicidae)

Consistent signatures of urban adaptation in a native, urban invader ant Tapinoma sessile

Increased acclimation ability accompanies a thermal niche shift of a recent invasion

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