Identification of Microsatellite Loci Associated With Embryogenic Potential in Wheat Genotypes in Anther Culture in Vitro

Abstract

In order to identify microsatellite loci that can be used as markers in screening for responsiveness in the in vitro
 anther culture, the efficiency of androgenesis in vitro was evaluated in 24 wheat genotypes. The forms contrasting in responsiveness in the in vitro anther culture were identified, and four F2 segregating populations were obtained on their basis. The study of interlinear polymorphism by 20 microsatellite loci located on chromosomes 5A and 5B was carried out. It was found that the cleavage by the ability to embryogenesis coincides with the cleavage by the alleles of the Xgwm371 locus: the 169 bp allele is associated with the ability to embryogenesis; the 185 bp allele — with the absence of such an ability or extremely low embryoid yield in the anther culture in vitro.