Identification of microRNAs involved in sucrose accumulation in sugarcane (Saccharum species hybrid)

Banerjee, Nandita; Singh, Alok; Annadurai, A.; Thirugnanasambandam, Prathima P.; Kumar, Sanjeev

doi:10.1016/j.plgene.2022.100352

Cited by 9 publications

(3 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As vacuole has hostile environment against SI, SUC remains safe from SI function and adds additional TH in total SRP. Cytoplasmic SUC isomerized into TH (major) and IM (minor) by the action of combined modified promoters driven ThSyGII gene 47,47 . Preliminary growth of sugarcane lines in small pots under small greenhouse conditions was at lower degrees as compared to first vegetative growth from setts in the open field conditions.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of healthful novel sugar contents in genetically engineered sugarcane juice integrated with molecularly characterized ThSyGII (CEMB-SIG2)

Awan

Ali

Iqbal

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Enhancement of sugar contents and yielding healthful sugar products from sugarcane demand high profile scientific strategies. Previous efforts to foster manipulation in metabolic pathways or triggering sugar production through combating abiotic stresses fail to yield high sugar recovery in Saccharum officinarum L. Novel sucrose isomers trehalulose (TH) and isomaltulose (IM) are naturally manufactured in microbial sources. In pursuance of novel scientific methodology, codon optimized sucrose isomerase gene, Trehalulose synthase gene II(CEMB-SIG2) cloned under dual combined stem specific constitutive promoters in pCAMBIA1301 expression vector integrated with Vacuole targeted signal peptide (VTS) to concentrate gene product into the vacuole. The resultant mRNA expression obtained by Real Time PCR validated extremely increased transgene expression in sugarcane culms than leaf tissues. Overall sugar estimation from transgenic sugarcane lines was executed through refractometer. HPLC based quantifications of Trehalulose (TH) alongside different internodes of transgenic sugarcane confirmed the enhancement of boosted sugar concentrations in mature sugarcane culms. Trehalulose synthase gene II receptive sugarcane lines indicated the unprecedented impressions of duly combined constitutive stem regulated promoters. Transgenic sugarcane lines produce highest sugar recovery percentages, 14.9% as compared to control lines (8.5%). The increased sugar recovery percentage in transgenic sugarcane validated the utmost performance and expression of ThSyGII gene .High Profile Liquid chromatography based sugar contents estimation of Trehalulose (TH) and Isomaltulose (IM) yielded unprecedented improvement in the whole sugar recovery percentage as compared to control lines.⁠

show abstract

Section: Discussionmentioning

confidence: 99%

Enhancement of healthful novel sugar contents in genetically engineered sugarcane juice integrated with molecularly characterized ThSyGII (CEMB-SIG2)

Awan

Ali

Iqbal

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Diacylglycerol kinase (DGK) can promote the production of phosphatidic acid under stress, promote ABA-mediated stomatal closure, and weaken photosynthesis [32]. In recent years, few miRNAs targeting DGK have been found in plants [32]. This study revealed that an unknown miRNA (1d_104_371678191_371678211) may target DGK, indicating that the weak photosynthetic capacity of Zhoumai 18 plants under drought conditions may be directly regulated by some unknown miRNAs.…”

Section: Mirna-triggered Chlorophyll Degradation Affects Photosynthet...mentioning

confidence: 97%

“…This study revealed that an unknown miRNA (3B_11_22107614_22107634) in the wheat variety Zhoumai 18 may target the FBA-encoding gene and downregulate its expression. Diacylglycerol kinase (DGK) can promote the production of phosphatidic acid under stress, promote ABA-mediated stomatal closure, and weaken photosynthesis [32]. In recent years, few miRNAs targeting DGK have been found in plants [32].…”

Section: Mirna-triggered Chlorophyll Degradation Affects Photosynthet...mentioning

confidence: 99%

Differential Transcription Profiling Reveals the MicroRNAs Involved in Alleviating Damage to Photosynthesis under Drought Stress during the Grain Filling Stage in Wheat

Zhou,

Song,

Xue

et al. 2024

IJMS

View full text Add to dashboard Cite

To explore the possible novel microRNA (miRNA) regulatory pathways in Zhengmai 1860, a newly cultivated drought-tolerant wheat (Triticum aestivum L.) cultivar, miRNA transcriptome sequencing of the flag leaves of Zhengmai 1860, drought-sensitive variety Zhoumai 18, and drought-resistant variety Bainong 207 was performed during the grain filling stage. We also observed changes in the chloroplast ultrastructure, phytohormone levels, and antioxidant- and photosynthesis-related physiological indicators in three wheat varieties. The results showed that the flag leaves of the drought-tolerant variety Zhengmai 1860 had higher chlorophyll contents and net photosynthetic rates than those of Zhoumai 18 under drought stress during the grain filling stage; in addition, the chloroplast structure was more complete. However, there was no significant difference between Zhengmai 1860 and Bainong 207. MiRNA transcriptome analysis revealed that the differential expression of the miRNAs and mRNAs exhibited variable specificity. The KEGG pathway enrichment results indicated that most of the genes were enriched in the MAPK signaling pathway, plant hormone signal transduction, photosynthetic antennae protein, and amino acid and carbohydrate metabolism. In the drought-tolerant cultivar Zhengmai 1860, tae-miR408 was targeted to regulate the allene oxide synthase (AOS) gene, inhibit its expression, reduce the AOS content, and decrease the synthesis of jasmonic acid (JA) and abscisic acid (ABA). The results of this study suggest that Zhengmai 1860 could improve the photosynthetic performance of flag leaves by inhibiting the expression of genes involved in the JA pathway through miRNAs under drought conditions. Moreover, multiple miRNAs may target chlorophyll, antioxidant enzymes, phytohormone signal transduction, and other related pathways; thus, it is possible to provide a more theoretical basis for wheat molecular breeding.

show abstract

Uncovering Alternate Splicing Events and Premature Stop Codons Associated with Early Sucrose Accumulation in Sugarcane Using BSR-seq

Banerjee,

Kumar,

Durai

et al. 2023

Plant Mol Biol Rep

View full text Add to dashboard Cite

Identification of microRNAs involved in sucrose accumulation in sugarcane (Saccharum species hybrid)

Cited by 9 publications

References 77 publications

Enhancement of healthful novel sugar contents in genetically engineered sugarcane juice integrated with molecularly characterized ThSyGII (CEMB-SIG2)

Enhancement of healthful novel sugar contents in genetically engineered sugarcane juice integrated with molecularly characterized ThSyGII (CEMB-SIG2)

Differential Transcription Profiling Reveals the MicroRNAs Involved in Alleviating Damage to Photosynthesis under Drought Stress during the Grain Filling Stage in Wheat

Uncovering Alternate Splicing Events and Premature Stop Codons Associated with Early Sucrose Accumulation in Sugarcane Using BSR-seq

Contact Info

Product

Resources

About