Identification of microRNA Targeting Cancer Gene of Colorectal Carcinoma in Caucasian Population

Bernard, Stefanus; Agustriawan, David

doi:10.1109/icoiact46704.2019.8938488

Cited by 6 publications

(3 citation statements)

References 10 publications

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“…Globally, CRC is the second and third leading causes of death in women and men, respectively (20). The aim of cancer therapy is to inhibit proliferation (cytostatic effects) and to induce cell death (cytotoxic effects) of cancer cells (21), to achieve an effective outcome with minimal side effects (23).…”

Section: Discussionmentioning

confidence: 99%

The Combined Effect of Dichloroacetate and 3-Bromopyruvate in Colorectal Cancer Cell Line, HT-29; The Mitochondrial Pathway Apoptosis

Nikravesh

Khodayar

Behmanesh

et al. 2021

Preprint

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Background 5-Fluorouracil (5-FU) is regarded as the first line treatment for colorectal cancer; however, its effectiveness is limited by drug resistance. The ultimate goal of cancer therapy is induction of cancer cell death to achieve an effective outcome with minimal side effects. The present work aimed to assess the anti-cancer activities of mitocans which can be considered as an effective anticancer drug due to high specificity in targeting cancer cells. Methods MTT (3–4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide) assay was performed to determine the effects of our mitocans on cell viability and cell death. Apoptosis and necrosis, caspase 3 activity, mitochondrial membrane potential and ROS production in HT29 cell lines were analyzed by ApopNexin™ FITC/PI Kit, Caspase- 3 Assay Kit, MitoTracker Green and DCFH-DA, respectively. Moreover, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression level of pro-apoptotic (Bax) and anti-apoptotic (Bcl-2) genes in HT29 cell lines. Results treatment with mitocans (3Br-P + DCA) inhibited the growth of HT29. Moreover, 3Br-P + DCA significantly induced apoptosis and necrosis, activation of caspase 3 activity, depolarize the mitochondrial membrane potential, and ROS production. At a molecular level, 3Br-P + DCA treatment remarkably down-regulated the expression of Bcl-2, while up-regulated the expression of Bax. Conclusion mitocans, in particular the combined drug, 3Br-P + DCA, could be regarded and more evaluated as a safe and effective compound for CRC treatment. Targeting hexokinase and pyruvate dehydrogenase kinase enzymes may be an option to overcome 5-FU -mediated chemo-resistant in colorectal cancer.

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Section: Discussionmentioning

confidence: 99%

The Combined Effect of Dichloroacetate and 3-Bromopyruvate in Colorectal Cancer Cell Line, HT-29; The Mitochondrial Pathway Apoptosis

Nikravesh

Khodayar

Behmanesh

et al. 2021

Preprint

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“…The methodology utilized was based on previous papers with detection of gene expressions through the steps of retrieving the data, processing the data, and creating a differential analysis from the expressions (Bernard and Agustriawan, 2019;Ivan, Patricia et al, 2021).…”

Section: Methodsmentioning

confidence: 99%

ANALISIS GEN KOMPARATIF KARSINOMA SEL SKUAMOSA PARU-PARU ANTARA INDIVIDU MEROKOK DAN TIDAK MEROKOK [Comparative Gene Analysis of Squamous Cell Lung Carcinoma Between Smoking and Non-smoking Individuals]

Shemuel,

Angelique,

Josephine

et al. 2023

Berita Biologi

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Squamous cell lung carcinoma (SCC) is a form of non-small cell lung cancer that commonly arises in the primary airway. The development of SCC is closely linked to changes in squamous cells that line the airways, primarily caused by exposure to tobacco smoke. To gain insights into SCC, bioinformatics techniques have been employed to detect biomarkers and analyze gene expression patterns, utilizing data from the Cancer Genome Atlas (TCGA) database, which was preprocessed for analysis. By employing DESeq2, a differential gene expression analysis method, identified genes showed significant variations in expression between smoking and non-smoking groups among the 11,530 genes examined. Notably, five genes, namely CT45A1, GCGR, TPTE, ABCC2, and PI16, were found to play a significant role in tumor development and were susceptible to under- or over-expression due to smoking. The majority of these genes were found to be underexpressed rather than overexpressed. These identified genes hold potential as biomarkers for tumor development and exhibit a strong correlation between smoking history and the development of SCC. However, a limitation encountered during this analysis was the unavailability of data from normal non-tumor patients, which could have facilitated a more comprehensive analysis of differential gene expression. Furthermore, this research gives a deeper implementation regarding the molecular mechanisms and genomics underlying SCC development, identifies differentially expressed genes associated with SCC and smoking history, and highlights potential biomarkers that warrant further investigation.

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“…Moreover, the resistance tendency of certain population group against malaria parasite has been observed as well [32]. Cancer is one of the most extensively studied disease in the pharmacogenomics field, in the light of the intensive computational biology tools, as tendencies for prevalence were elucidated in different races [33,34]. Thus, the progress of epigenetics research has leveraged the pharmacogenomics field with various findings of biomarkers for cancer [35].…”

Section: Pharmacogenomics and Personalized Medicinementioning

confidence: 99%

Introductory Chapter: The Emerging Corner of the Omics Studies for Rational Drug Design

Parikesit¹

2021

Drug Design - Novel Advances in the Omics Field and Applications

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Identification of microRNA Targeting Cancer Gene of Colorectal Carcinoma in Caucasian Population

Cited by 6 publications

References 10 publications

The Combined Effect of Dichloroacetate and 3-Bromopyruvate in Colorectal Cancer Cell Line, HT-29; The Mitochondrial Pathway Apoptosis

The Combined Effect of Dichloroacetate and 3-Bromopyruvate in Colorectal Cancer Cell Line, HT-29; The Mitochondrial Pathway Apoptosis

ANALISIS GEN KOMPARATIF KARSINOMA SEL SKUAMOSA PARU-PARU ANTARA INDIVIDU MEROKOK DAN TIDAK MEROKOK [Comparative Gene Analysis of Squamous Cell Lung Carcinoma Between Smoking and Non-smoking Individuals]

Introductory Chapter: The Emerging Corner of the Omics Studies for Rational Drug Design

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