Identification of METTL14 in Kidney Renal Clear Cell Carcinoma Using Bioinformatics Analysis

Abstract

The kidney renal clear cell carcinoma (KIRC) with poor prognosis is the main histological subtype of the renal cell carcinoma, accounting for 80–90% of patients. Currently, the N6-methyladenosine (m6A) epitranscriptional modification draws much attention. The m6A RNA modification, the most plentiful internal modification of mRNAs and noncoding RNAs in the majority of eukaryotes, regulates mRNAs at different levels and is involved in disease occurrence and progression. The GTExPortal and TCGAportal were applied to investigate the METTL14 mRNA expression in different tissues and KIRC stages. The Human Protein Atlas was used to verify the location of METTL14 in KIRC tissues. The main microRNAs (miRNAs) related to KIRC were analyzed using OncoLnc and starBase, while corresponding circular RNAs (circRNAs) interacting with miRNAs were predicted via circBank; then, the METTL14-miRNA-circRNA interaction network was established. The level of methyltransferase-like 14 (METTL14) mRNA was significantly lower in KIRC tissues compared with normal kidney tissues, which was relative to clinical and pathological stages. circRNAs may regulate METTL14 mRNA as miRNAs sponge to affect the KIRC progression. METTL14 mRNA is likely to regulate PTEN mRNA expression via changing its m6A RNA modification level. METTL14 mRNA expression negatively correlated with the KIRC stages and positively correlated with KIRC patients’ overall survival, which has great potential to serve as a clinical biomarker in KIRC.

Role of methyltransferase-like enzyme 3 and methyltransferase-like enzyme 14 in urological cancers

“…Synergistic effects may occur through the interaction of EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A) and METTL14, which regulates kidney cancer progression. In addition, 24 circRNAs (e.g., circ-0023414 and circ-0031772) interact with four miRNAs (miR-130a-3p, miR-106b-5p, miR-130b-3p, and miR-301a-3p), which have a negative relationship with METTL14 mRNA (Wang et al, 2019). These circRNAs may act as miRNA sponges to regulate METTL14 mRNA ( Fig.…”

Comprehensive analysis on the expression levels and prognostic values of LOX family genes in kidney renal clear cell carcinoma

“…28 In recent years, molecular biology technology and bioinformatics have rapidly developed. Some potential RCC therapeutic targets have been identified using bioinformatics, such as ALOX5, METTL14, et al 29,30 For the first time, we used multiple online databases to comprehensively investigate the expression levels of the LOX family genes in KIRC at the levels of tumor tissue mRNA, tumor tissue protein and RCC cell lines mRNA, and further explored their significance on prognosis and molecular mechanism in KIRC. LOX family genes have been shown to play key roles in the formation and remodeling of ECM, which could affect biological processes such as cell differentiation, proliferation, adhesion, morphogenesis and phenotypic expression.…”

Peer Review #1 of "Role of methyltransferase-like enzyme 3 and methyltransferase-like enzyme 14 in urological cancers (v0.1)"

“…Manuscript to be reviewed 106b-5p, miR-130b-3p, and miR-301a-3p), which have a negative relationship with METTL14 mRNA (Wang et al 2019). These circRNAs may act as miRNA sponges to regulate METTL14 mRNA ( Fig.1).…”