Identification of metal species by ESI-MS/MS through release of free metals from the corresponding metal-ligand complexes

Abstract

Electrospray ionization-mass spectrometry (ESI-MS) is used to analyze metal species in a variety of samples. Here, we describe an application for identifying metal species by tandem mass spectrometry (ESI-MS/MS) with the release of free metals from the corresponding metal–ligand complexes. The MS/MS data were used to elucidate the possible fragmentation pathways of different metal–deoxymugineic acid (–DMA) and metal–nicotianamine (–NA) complexes and select the product ions with highest abundance that may be useful for quantitative multiple reaction monitoring. This method can be used for identifying different metal–ligand complexes, especially for metal species whose mass spectra peaks are clustered close together. Different metal–DMA/NA complexes were simultaneously identified under different physiological pH conditions with this method. We further demonstrated the application of the technique for different plant samples and with different MS instruments.

Synthesis of Fluorescent 1,7-Dipyridyl-bis-pyrazolo[3,4-b:4′,3′-e]pyridines: Design of Reversible Chemosensors for Nanomolar Detection of Cu2+

“…The ion peak at m / z 255.0558, with an intensity of 100%, corresponds to the dication species [ 6a + Cu 2+ ] 2+ (calcd, 255.0546), and the isotopic peak pattern supports the presence of copper. 35 This experiment also allowed us to detect an ion peak at m / z 572.0994, with an intensity of 78%, which is assigned to the monocation species [ 6a + CuNO 3 + ] + (calcd, 572.0976); see Figures S9–S11 for details (Supporting Information).…”

Zinc Homeostasis and isotopic fractionation in plants: a review

“…Additionally, Zn 2+ can diffuse from one cell to another across the plasmodesmata, small openings in the cell walls that allow the cytoplasm of adjacent cells to communicate. This is a kinetically controlled process that will favour the light isotopes (Criss 1999 (Xuan et al 2006), and in the shoots of japonica rice (Tsednee et al 2016), which suggests that Zn could be transported up the xylem as Zn-PS complexes. All of these steps might contribute to the isotope ratios of roots and shoots.…”

Identification of Metallophores and Organic Ligands in the Chemosphere of the Marine Macroalga Ulva (Chlorophyta) and at Land-Sea Interfaces

“…Using this algorithm, Baars and co-workers could also determine novel metallophores in the culture medium of A. vinelandii (Baars et al, 2016). In addition, ESI-MS/MS experiments were suggested to identify metal-ligand complexes through the release of metal species from its respective complex (Tsednee et al, 2016).…”