Identification of metabolizing enzyme genes associated with xenobiotics and odorants in the predatory stink bug Arma custos based on transcriptome analysis

Li, Wenhong; Zou, Jingmiao; Xiang, Yang; Yang, Ming-Wei; Jiang, Po; Wang, Xinyi; Huang, Chunyang; He, Yan

doi:10.1016/j.heliyon.2023.e18657

Cited by 3 publications

(3 citation statements)

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“…The predatory stinkbug rely on its antennae to detect various chemical signals, and living prey typically generate movement and vibrations, which can attract the attention of predatory stinkbugs seeking suitable prey. 63 More importantly, the TTP/SPc complex showed no significant stomach or contact toxicity against the predatory stinkbug. The insensitivity of the predatory stinkbug to TTP may be due to the binding site difference between the pest and predator, and TTP has a unique chemical structure and displays excellent activity against lepidopterans.…”

Section: Discussionmentioning

confidence: 98%

Construction of a nontoxic nano-pesticide and its co-application with natural predators for perfect cooperative pest management: an innovative strategy for pesticide reduction

Wu,

Jiang,

Huang

et al. 2024

Environ. Sci.: Nano

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Section: Discussionmentioning

confidence: 98%

Construction of a nontoxic nano-pesticide and its co-application with natural predators for perfect cooperative pest management: an innovative strategy for pesticide reduction

Wu,

Jiang,

Huang

et al. 2024

Environ. Sci.: Nano

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“…The tBLASTn research were performed to predict the genes of neuropeptide precursors and their GPCR receptors from our custom A. custos transcriptome (BioProject No. PRJNA878928, heads, guts, and salivary glands from 100 fifth instar nymphs starved for about 12 h prior; three biological replicates; RNA-seq) (Li et al, 2023). Amino acid sequences of known neuropeptide precursors from H. halys (Lavore et al, 2018), N. lugens (Tanaka et al, 2014), and R. prolixus (Ons et al, 2011), and neuropeptide GPCRs from N. viridula (Lavore et al, 2018), A. lucorum (Gao et al, 2021), R. prolixus (Ons et al, 2011), and N. lugens (Tanaka et al, 2014) were used as reference queries.…”

Section: Identification Of Neuropeptides and Their Gpcrsmentioning

confidence: 99%

“…It is widely distributed in China and other Asian regions and frequently found on various trees and crops (Zou et al, 2012). A. custos has been successfully massly bred by a number of natural enemy factories in China and has been released in tobacco field to control some important pest insects, such as Spodoptera frugiperda and Helicoverpa assultam (Li et al, 2023). In-depth understanding of biology, ecology, and physiology of A. custos could help us to better utilize these biological control agents.…”

Section: Introductionmentioning

confidence: 99%

Identification of neuropeptides and their G protein‐coupled receptors in the predatory stink bug, Arma custos (Hemiptera: Pentatomidae)

Huang,

Dong,

Yang

et al. 2024

Arch Insect Biochem Physiol

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The predatory stink bug Arma custos has been selected as an effective biological control agent and has been successfully massly bred and released into fields for the control of a diverse insect pests. As a zoophytophagous generalist, A. custos relies on a complex neuropeptide signaling system to prey on distinct food and adapt to different environments. However, information about neuropeptide signaling genes in A. custos has not been reported to date. In the present study, a total of 57 neuropeptide precursor transcripts and 41 potential neuropeptide G protein‐coupled receptor (GPCR) transcripts were found mainly using our sequenced transcriptome data. Furthermore, a number of neuropeptides and their GPCR receptors that were enriched in guts and salivary glands of A. custos were identified, which might play critical roles in feeding and digestion. Our study provides basic information for an in‐depth understanding of biological and ecological characteristics of the predatory bug and would aid in the development of better pest management strategies based on the effective utilization and protection of beneficial natural enemies.

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Insecticidal activities of three recombinant venom proteins of the predatory stink bug, Arma custos

Wang,

Li,

Yang

et al. 2024

Pest Management Science

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BACKGROUNDWidespread resistance of insect pests to insecticides and transgenic crops in the field is a significant challenge for sustainable agriculture, and calls for the development of novel alternative strategies to control insect pests. One potential resource for the discovery of novel insecticidal molecules is natural toxins, particularly those derived from the venoms of insect predators.RESULTSIn this study, we identified three insecticidal proteinaceous toxins from the venom glands (VGs) of the predatory stink bug, Arma custos (Hemiptera: Asopinae). Transcriptomic analysis of A. custos VGs revealed 151 potentially secreted VG‐rich venom proteins. Three VG‐rich venom proteins (designated AcVP1 ~ 3) were produced by overexpression in Escherichia coli. Injection of the recombinant proteins into tobacco cutworm (Spodoptera litura) larvae showed that all of the three recombinant proteins caused paralysis, liquefaction and death. Injection of recombinant proteins into rice brown planthopper (Nilaparvata lugens) nymphs showed higher insecticidal activities, among which a trypsin (AcVP2) caused 100% mortality postinjection at 1.27 pmol mg−1 body weight.CONCLUSIONA natural toolkit for the discovery of insecticidal toxins from predatory insects has been revealed by the present study. © 2024 Society of Chemical Industry.

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Identification of metabolizing enzyme genes associated with xenobiotics and odorants in the predatory stink bug Arma custos based on transcriptome analysis

Cited by 3 publications

References 69 publications

Construction of a nontoxic nano-pesticide and its co-application with natural predators for perfect cooperative pest management: an innovative strategy for pesticide reduction

Construction of a nontoxic nano-pesticide and its co-application with natural predators for perfect cooperative pest management: an innovative strategy for pesticide reduction

Identification of neuropeptides and their G protein‐coupled receptors in the predatory stink bug, Arma custos (Hemiptera: Pentatomidae)

Insecticidal activities of three recombinant venom proteins of the predatory stink bug, Arma custos

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