Identification of metabolites present in Opuntia callus and study of their antioxidant, anti-inflammatory and anti-adipogenic properties

Camarena-Rangel, Nancy Gabriela; Antunes‐Ricardo, Marilena; Gutiérrez-Uribe, Janet A.; Velarde‐Salcedo, Aída Jimena; Rosa, Ana Paulina Barba de la; Santos-Díaz, María del Socorro

doi:10.1007/s11240-020-01893-4

Cited by 8 publications

(5 citation statements)

References 47 publications

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“…The amount of work required to bring a unit positive charge from infinity to the surface without acceleration is known as the electric potential of a surface [ 30 ]. Zeta potential is related to pharmacokinetic properties in the body and the facility of nanoparticle phagocytosis in the bloodstream or cell [ 31 ]. The results of the zeta potential of each nanoparticle ( Table 2 ), NEQs, NBs, and NPs were significantly different compared to each one.…”

Section: Resultsmentioning

confidence: 99%

Novel Hybrid Inulin–Soy Protein Nanoparticles Simultaneously Loaded with (-)-Epicatechin and Quercetin and Their In Vitro Evaluation

et al. 2023

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(-)-Epicatechin and quercetin have attracted considerable attention for their potential therapeutic application in non-communicable chronic diseases. A novel hybrid inulin–soy protein nanoparticle formulation was simultaneously loaded with (-)-epicatechin and quercetin (NEQs) to improve the bioavailability of these flavonoids in the human body, and NEQs were synthesized by spray drying. After process optimization, the physicochemical and functional properties of NEQs were characterized including in vitro release, in vitro gastrointestinal digestion, and cell viability assays. Results showed that NEQs are an average size of 280.17 ± 13.42 nm and have a zeta potential of −18.267 ± 0.83 mV in the organic phase. Encapsulation efficiency of (-)-epicatechin and quercetin reached 97.04 ± 0.01 and 92.05 ± 1.95%, respectively. A 3.5% soy protein content conferred controlled release characteristics to the delivery system. Furthermore, NEQs presented inhibitory effects in Caco-2, but not in HepG-2 and HDFa cell lines. These results contribute to the design and fabrication of inulin–soy protein nanoparticles for improving the bioavailability of multiple bioactive compounds with beneficial properties.

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Section: Resultsmentioning

confidence: 99%

Novel Hybrid Inulin–Soy Protein Nanoparticles Simultaneously Loaded with (-)-Epicatechin and Quercetin and Their In Vitro Evaluation

et al. 2023

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“…Absorbance measures were performed at 550 nm according to the manufacturer’s instructions using a microplate reader (Synergy HT, Bio-Tek, Winooski, VT, USA). The results were expressed as a percentage of NO production [ 33 ], and obtained in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Optimization of the Synthesis of Natural Polymeric Nanoparticles of Inulin Loaded with Quercetin: Characterization and Cytotoxicity Effect

et al. 2022

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Quercetin is a bioactive component that is capable of having therapeutic potential in the prevention of different noncommunicable chronic diseases (NCDs). However, it presents instability in the gastrointestinal tract in addition to low bioavailability. One way to overcome the limitations of quercetin lies in using nanotechnology for the development of nanoparticles, based on biopolymers, that are capable of being ingestible. Inulin, a fructan-type polysaccharide, acts as a delivery system for the release of quercetin in a target cell, guaranteeing the stability of the molecule. Inulin-coated quercetin nanoparticles were synthesized by the spray dryer method, and four variables were evaluated, namely inulin concentration (5–10% w/v), feed temperature (40–60 °C), inlet temperature (100–200 °C) and outlet temperature (60–100 °C). The optimal conditions were obtained at 10% w/v inulin concentration, with 45 °C feed temperature, 120 °C inlet temperature and 60 °C outlet temperature, and the nanoparticle size was 289.75 ± 16.3 nm in water. Fluorescence microscopy indicated quercetin loading in the inulin nanoparticles, with an encapsulation efficiency of approximately 73.33 ± 7.86%. Inulin-coated quercetin nanoparticles presented effects of inhibition in Caco-2 and HepG2 cells, but not in HDFa cells. The experimental data showed the potential of inulin nanoparticles as transport materials for unstable molecules, in oral administration systems, for the encapsulation, protection and release of quercetin.

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“…The groups that received the Hypercaloric diet (H) and the Opuntia ficus-indica flour (HO) had greater adipose tissue mass (Epididymal and Visceral), differing from other studies whose cactaceae promoted a decrease in adipose tissue mass or size of adipocytes. Glycosides from Opuntia ficus-indica decreased the size of adipocytes in rats (Rodríguez-Rodríguez et al, 2015), the anti-adipogenic effect of Opuntia ficus-indica and other plants of the genus Opuntia was also demonstrated in 3T3-L1 cells, in which the reduction of lipid concentration in cells after treatment with cactaceae (Camarena-Rangel et al, 2020). Consumption of a high-fat diet with 4% fiber from dehydrated Opuntia ficus-indica decreased body fat and adipocyte hypertrophy in rats (Moran-Ramos et al, 2017).…”

Section: Liver Weight and Adipose Tissue Massmentioning

confidence: 99%

Morphometric parameters and adipose tissue mass of rats fed with cactaceae flour

Ferreira,

Barakat,

Almeida

et al. 2023

Acta Sci. Anim. Sci.

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The aim of this study was to evaluate the morphometric parameters and the mass of adipose tissues of rats fed with cactacea flour. The animals were divided into five groups: C (Control), H (Hypercaloric diet), HP (Hypercaloric diet with 5% Pereskia grandifolia flour), HO (Hypercaloric diet with 5% Opuntia ficus-indica flour), and HA (Hypercaloric diet with 5% Agave tequilana flour). Feed intake and apparent digestibility, capillary glycemia, liver weight and Hepato-Somatic Index were evaluated. Adipose tissue mass and the Visceral Fat Index (VFI) and Epididymal Fat Index (EFI) were determined. The data were compared using the Tukey test at 5% significance level. There was no statistical difference between the groups for body weight, BMI and Lee Index. The HP and HA groups did not differ from the C group regarding weight gain, the C group had lower apparent digestibility of the diets, the HA group presented lower blood glucose when compared to the H and HO groups, and the H group had higher liver weight. The HP and HA groups gained the lowest adipose tissue mass, VFI and EFI than the H and HO groups. Among the cactaceae studied, Pereskia grandifolia showed better effects on morphometric and adipose tissue parameters.

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Identification of metabolites present in Opuntia callus and study of their antioxidant, anti-inflammatory and anti-adipogenic properties

Cited by 8 publications

References 47 publications

Novel Hybrid Inulin–Soy Protein Nanoparticles Simultaneously Loaded with (-)-Epicatechin and Quercetin and Their In Vitro Evaluation

Novel Hybrid Inulin–Soy Protein Nanoparticles Simultaneously Loaded with (-)-Epicatechin and Quercetin and Their In Vitro Evaluation

Optimization of the Synthesis of Natural Polymeric Nanoparticles of Inulin Loaded with Quercetin: Characterization and Cytotoxicity Effect

Morphometric parameters and adipose tissue mass of rats fed with cactaceae flour

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