Identification of metabolite traits from the current metabolomic approaches

Omena-García, Rebeca P.; Silva, Lucas de Ávila; Vital, Camilo Elber; Araújo, Wagner L.; Ramos, Humberto J.O.; Nunes‐Nesi, Adriano

doi:10.1007/s40626-019-00147-9

Cited by 3 publications

(3 citation statements)

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“…For plant breeders, linking these resistance traits to genetic markers is essential. The common approaches are QTL analysis or GWAS mapping [ 138 ]. With GWAS, natural accessions are genotyped by sequencing.…”

Section: Metabolomics Research In Plant—pest Resistance Breedingmentioning

confidence: 99%

“…These same accessions are subjected to metabolomics, which can be combined with QTL maps, yielding information on the genomic regions that certain metabolites, or mQTL, are associated with. Resistance factors have been identified by relating the gene sequence with the metabolome of the individuals [ 138 ]. Both mapping methods combined with metabolomics will identify accessions that are associated with resistance and particular metabolic traits.…”

Section: Metabolomics Research In Plant—pest Resistance Breedingmentioning

confidence: 99%

“…Both mapping methods combined with metabolomics will identify accessions that are associated with resistance and particular metabolic traits. Only by further genetic fine mapping, the responsible regulatory or biosynthetic genes can be identified [ 138 , 139 ]. In addition, these approaches could be combined with targeted genome editing tools, such as CRISPR-Cas9, which precisely target specific DNA sequences [ 140 ].…”

Section: Metabolomics Research In Plant—pest Resistance Breedingmentioning

confidence: 99%

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Unravelling Plant Responses to Stress—The Importance of Targeted and Untargeted Metabolomics

et al. 2021

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Climate change and an increasing population, present a massive global challenge with respect to environmentally sustainable nutritious food production. Crop yield enhancements, through breeding, are decreasing, whilst agricultural intensification is constrained by emerging, re-emerging, and endemic pests and pathogens, accounting for ~30% of global crop losses, as well as mounting abiotic stress pressures, due to climate change. Metabolomics approaches have previously contributed to our knowledge within the fields of molecular plant pathology and plant–insect interactions. However, these remain incredibly challenging targets, due to the vast diversity in metabolite volatility and polarity, heterogeneous mixtures of pathogen and plant cells, as well as rapid rates of metabolite turn-over. Unravelling the systematic biochemical responses of plants to various individual and combined stresses, involves monitoring signaling compounds, secondary messengers, phytohormones, and defensive and protective chemicals. This demands both targeted and untargeted metabolomics approaches, as well as a range of enzymatic assays, protein assays, and proteomic and transcriptomic technologies. In this review, we focus upon the technical and biological challenges of measuring the metabolome associated with plant stress. We illustrate the challenges, with relevant examples from bacterial and fungal molecular pathologies, plant–insect interactions, and abiotic and combined stress in the environment. We also discuss future prospects from both the perspective of key innovative metabolomic technologies and their deployment in breeding for stress resistance.

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Section: Metabolomics Research In Plant—pest Resistance Breedingmentioning

confidence: 99%

Section: Metabolomics Research In Plant—pest Resistance Breedingmentioning

confidence: 99%