Identification of metabolite compounds from ethanolic extract of the Red Gedi Leaves (Abelmoschus manihot L. Medik) by LC-ESI-MS

Tangka, Juliet; Barung, Elisabeth Natalia; Lyrawati, Diana; Soeatmadji, Djoko Wahono; Nurdiana, Nurdiana

doi:10.52711/0974-360x.2022.00869

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…Medik, known as "gedi," was classified as a medicinal herb (1,2) . Abelmoschus manihot (L.) Medik can be a complementary treatment to reduce blood sugar and blood pressure and work as an anti-inflammatory (3) .…”

Section: The Tropical Plant Abelmoschus Manihot Lmentioning

confidence: 99%

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The inhibition of α-Glucosidase enzyme activity from Standardised Ethanol Extract of Abelmoschus manihot (L.) Medik Leaves

Prasetiyo,

Winarti,

Agustia

2023

jifi

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People have long utilised the leaves of the red gedi, Abelmoschus manihot (L.) Medik is an anti-diabetic medication. The study’s objectives were to classify chemical constituent groups, assess extract quality, and evaluate the activity of α-glucosidase enzyme inhibitors. The extraction method employed kinetic maceration with a 70% ethanol solvent, identification was accomplished through phytochemical screening, a quality determination was based on both specific and non-specific parameters, and α-glucosidase inhibition was used in an in vitro antidiabetic activity test. Flavonoids, alkaloids, saponins, steroids, and triterpenoids were found in the phytochemical screening test results. The results of the quality parameter test obtained viscous extract, blackish green color, 70.58% water soluble extract, 49.46% ethanol soluble extract, 7.82% loss on drying, 4.19% water content, total ash content 6.97%, acid insoluble ash content 0.60%, water-soluble ash content 5.64%, residual solvent 0.76%, heavy metal content Pb 1.35 mg/kg, Cd 0.06 mg/kg, plate number total ≤10 colonies/g, yeast plate number 20.1995 colonies/g and total flavonoid content 1.20%. The α-glucosidase enzyme inhibitory activity test from the extract and acarbose (positive controls) obtained IC50 values of 81.84 μg/mL and 42.6 μg/mL, respectively. In conclusion, the α-glucosidase enzyme may be inhibited by the ethanolic extract of A. manihot (L.) Medik leaves.

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Section: The Tropical Plant Abelmoschus Manihot Lmentioning

confidence: 99%

“…A. manihot (L.) Medik leaves may contain a secondary metabolite that can be used as a source for standardised herbal medicines or phytopharmaceuticals, particularly for antidiabetics (1) . The extraction of raw materials was required for the standardisation of herbal medicine.…”

mentioning

confidence: 99%

The inhibition of α-Glucosidase enzyme activity from Standardised Ethanol Extract of Abelmoschus manihot (L.) Medik Leaves

Prasetiyo,

Winarti,

Agustia

2023

jifi

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DPP-IV Inhibitory Activity of the Ethanolic Extract of Red Gedi Leaves Abelmoschus manihot L. Medic

Tangka

Barung

Lyrawati

et al. 2022

Open Access Maced J Med Sci

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BACKGROUND: At present, there are many drugs used to manage diabetes including dipeptidyl peptidase-4 (DPP-IV) inhibitors which target insulin secretion. Abelmoschus manihot L. Medic, an endemic species of Minahasa, Indonesia, has been used as an antidiabetic herbal medicine. AIM: In this study, we studied its metabolites activities, in silico and in vitro, as inhibitor for DPP-IV, thus regulating insulin secretion. RESULTS: Of 38 identified metabolites, when docked into the catalytic site DPP-IV, 10 showed good binding energy within range of the standard gliptin drugs, that is, hibiscetin, gossypentin, gossypetin - 3-glucoside, myricetin, myricetin 3-glucoside, alpha spinasterol, quercetin, syringaresinol, stigmasterol, and isoquercetin. Three of those ten metabolites showed Ki within standard drugs values, that is, gossypetin, alpha spinasterol, and stigmasterol. The profile of molecular dynamic simulation, total energy and root mean square deviation of those metabolites were all similar with the standard gliptin drugs and predicted good stability of the complexes. The subsequent in vitro assay determining DPP-IV activity of the red Gedi leaves extract demonstrated that indeed the extract inhibited DPP-IV activity with IC50 860.67 μg/mL. Further studies are ongoing to prove the antidiabetic properties of the whole as well as isolated single compounds of the extract in particular gossypetin, alpha spinasterol, and stigmasterol as DPP-IV inhibitors. CONCLUSION: Our in silico studies showed that the compounds of ethanolic extract of red Gedi leaves potentially serve as DPP-IV inhibitors. Based on computed binding affinity, Ki, total energy, RMSD, and stability, the most potent compounds of the extract to inhibit DPP-IV activity are probably gossypetin, alpha spinasterol, and stigmasterol.

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Identification of metabolite compounds from ethanolic extract of the Red Gedi Leaves (Abelmoschus manihot L. Medik) by LC-ESI-MS

Cited by 2 publications

References 15 publications

The inhibition of α-Glucosidase enzyme activity from Standardised Ethanol Extract of Abelmoschus manihot (L.) Medik Leaves

The inhibition of α-Glucosidase enzyme activity from Standardised Ethanol Extract of Abelmoschus manihot (L.) Medik Leaves

DPP-IV Inhibitory Activity of the Ethanolic Extract of Red Gedi Leaves Abelmoschus manihot L. Medic

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