2004
DOI: 10.1152/physiolgenomics.00149.2004
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Identification of MEF2-regulated genes during muscle differentiation

Abstract: Although a great deal has been elucidated concerning the mechanisms regulating muscle differentiation, little is known about transcription factor-specific gene regulation. Our understanding of the genetic mechanisms regulating cell differentiation is quite limited. Much of what has been defined centers on regulatory signaling cascades and transcription factors. Surprisingly few studies have investigated the association of genes with specific transcription factors. To address these issues, we have utilized a me… Show more

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Cited by 22 publications
(18 citation statements)
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“…Using a custom CpG island array and a pan-MEF2 antibody, 20 genes were identified in C2C12 cells whose promoters were enriched for MEF2 binding (39). Of these, only nine overlapped with our microarray data and were found exclusively in the MEF2A gene set, suggesting that they may be direct targets of MEF2A.…”
Section: Discussionmentioning
confidence: 70%
“…Using a custom CpG island array and a pan-MEF2 antibody, 20 genes were identified in C2C12 cells whose promoters were enriched for MEF2 binding (39). Of these, only nine overlapped with our microarray data and were found exclusively in the MEF2A gene set, suggesting that they may be direct targets of MEF2A.…”
Section: Discussionmentioning
confidence: 70%
“…Identification and in Silico Analysis of dapr-In a previous study we combined chromatin immunoprecipitation and CpG microarray analysis to search for genes regulated by the muscle related transcription factor MEF2 during skeletal muscle differentiation (11). Using chromatin immunoprecipitation-PCR we confirmed MEF2 binding proximal to a number of genes, peaking 2 days after initiation of differentiation.…”
Section: Resultsmentioning
confidence: 90%
“…C, aliquots of each fraction were separated by gel electrophoresis and probed with antibodies to proteins located specifically to either the cytosol (␤-tubulin) or plasma membrane (Na/K ATPase). determine whether there were any novel genes through which expression was induced by MEF2 binding (11). Reconfirmation and in silico analysis led us to a previously unknown and unnamed gene, which we have called dapr.…”
Section: Discussionmentioning
confidence: 99%
“…54,55 Therefore, we measured MEF2-dependent transcription in C2C12 cells before and after serum starvation in the presence or absence of siRNA against CycT1. The interaction between endogenous MEF2 and P-TEFb was first confirmed by coimmunoprecipitation (Fig.…”
Section: Resultsmentioning
confidence: 99%