Identification of market adulterants in East Indian sandalwood using DNA barcoding

Dev, Suma Arun; Muralidharan, E. M.; Sujanapal, P.; Balasundaran, M.

doi:10.1007/s13595-013-0354-0

Cited by 30 publications

(18 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…UBC834 (AG) 8 YT, UBC874 (CCT) 4 , UBC848(CA) 8 RG, UBC857 (AC) 8 YG) ISSR primers amplified. The universal SSR markers rbcL (5'-AACACCAGCTTTRAATCCAA-3') and MatK (5'-GGGTTGCTAACTCAATGGTAGAG-3') primers [22] were used to validate the quality of extracted wood DNA. In this study, basically particular universal SSR primers which were used to carry out research in barcoding of timbers, which would be useful in illegal logging and tracing the timber origin.…”

Section: Dna Analysis Through Issr and Universal Ssr (Rbcl And Trnh) Mamentioning

confidence: 99%

An Effective Wood DNA Extraction Protocol for Three Economic Important Timber Species of India

Fatima¹,

Srivastava²,

Hanur³

et al. 2018

AJPS

View full text Add to dashboard Cite

Extraction of DNA from fresh tissues is routine in studies of tropical forest species, but DNA extraction from wood is considered as difficult due to its highly degraded nature and adequate quality of genomic DNA extraction is essential for molecular studies. Very few studies have validated the potential for isolating DNA from dried wood (Heartwood and Sapwood). Wood genomic DNA extraction is difficult from mature timber (Teak (Tectona grandis f; verbanaceae), Black Rosewood (Dalbergia latifolia f; Fabaceae) Ben Teak (Lagerstroemia lanceolata f; Lytheraceae) tissues due to presence of high quantity of secondary metabolites polyphenols, tannins and terpenoids and protein inhibitors. Mostly in laboratories DNA extraction kits are available but by using kits, DNA yield is very low and it is quite expensive too. We have standardized and validated the DNA extraction through manual protocol which is applicable for Bark, Sapwood and Heartwood samples of tree species which contains huge amount of inflexible tissues and fibers. The quality of the DNA was tested by spectrophotometer, gel electrophoresis and PCR (ISSR and SSR) amplification. An avrage DNA yield for heartwood ranges from 0.186 -0.166 µg/µL and sapwood was ranges from 0.26 -0.244 µg/µL. Modification of CTAB method was by addition of polyvinylpyrrolidone (PVP) appx 0.25%, variation in Rnase concentration, proteinase treatment with different concentration and incubation time. In order to evaluate the standardized wood genomic DNA extraction protocol, we compared it with the mature leaf and core samples (heartwood and sapwood) of the same timber species. The outcome was also quantified and proved by means of polymerase chain reaction analysis by using ISSR and SSR microsatellite markers conducted with isolated pure DNAs. This modified protocol made increased yield and purity of wood total genomic DNA and facilitate the important application of forenHow to cite this paper: Fatima, T., Srivastava, A., Hanur, V.S. and Srinivasa Rao, M. (2018) An Effective Wood DNA Extraction

show abstract

Section: Dna Analysis Through Issr and Universal Ssr (Rbcl And Trnh) Mamentioning

confidence: 99%

An Effective Wood DNA Extraction Protocol for Three Economic Important Timber Species of India

Fatima¹,

Srivastava²,

Hanur³

et al. 2018

AJPS

View full text Add to dashboard Cite

show abstract

“…The DNA barcoding has been identified as a promising tool to detect timber adulterants [26][27][28][29] . Dev et al 15 has employed DNA barcoding to authenticate Santalum album L. (East Indian sandalwood) samples in the States Karnataka, Kerala and Tamil Nadu of India. However, the present study is the first comprehensive report that provides survey information to identify the adulterant species of wood.…”

Section: Discussionmentioning

confidence: 99%

“…Because of the improvement of molecular biology and usage of automated machines that reduces the labor and the requirement of skills, DNA barcoding has become inexpensive 15 , making it more suitable for rapid identification of species. Although DNA barcoding can provide unique identity and implement in many fields in an inexpensive manner, it is not famous as a tracking method of timber adulterations.…”

mentioning

confidence: 99%

“…Although DNA barcoding can provide unique identity and implement in many fields in an inexpensive manner, it is not famous as a tracking method of timber adulterations. However, DNA barcoding is proven capable of detecting timber adulterations as reported for sandalwood 15 and monitor illegal logging of endangered species 16 . However, the challenging step is the extraction of good quality DNA from a small amount of dried or processed wood.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Assessment of the applicability of wood anatomy and DNA barcoding to detect the timber adulterations in Sri Lanka

Kannangara

Karunarathne

Ranaweera

et al. 2020

Sci Rep

View full text Add to dashboard Cite

the wood adulteration is a common problem and under-studied aspect in the timber industry of Sri Lanka. Hence we conducted a survey to assess the status of timber adulteration and check the applicability of morphometric parameters and DnA barcoding to detect the adulterated timber sources. We interviewed the stakeholders of the timber industry to collect information regarding timber adulterations. We measured the morphometric parameters; wood density and sizes of the xylem elements of the standard and adulterant species. for DnA barcoding, DnA was extracted from the wood of the selected standard and adulterant species and subjected to pcR using the markers, matK-trnT and atpB-rbcL. the pcR products were subjected to DnA sequencing. According to the survey, 92.5% of patrons, 73.7% of manufacturers and 96.7% of carpenters said timber adulteration is taking place in the country. the respondents said that the standard timber species; Tectona grandis, Artocarpus heterophyllus, and Swietenia macrophylla, profoundly undergo adulteration in Sri Lanka. the morphometric parameters did not discriminate the adulterant species from the standard species. the DnA barcodes matK-trnT and atpB-rbcL provided unique polymorphic DNA sequences with specific lengths for each species permitting the precise establishment of species identity and enabling the accurate detection of timber adulterations.

show abstract

“…DNA barcoding involves using a short, agreed-upon region of a genome as a unique identifier for species and provides identification of a specimen to species even if only a small fragment of plant material is available. Furthermore, the power and strength of DNA barcoding has been demonstrated in many botanical studies ranging from the discovery of unknown species, resolving taxonomic problems, species adulteration in herbal products (Srirama et al 2010;Baker et al 2012;Newmaster et al 2013), and TM market surveys (Kool et al 2012;Arun Dev et al 2014); DNA barcoding has also proven to be of great utility in scrutinizing the illegal trade of endangered plant species (Pryer et al 2010;Subedi et al 2013). However, the taxonomic value of DNA barcoding has rarely been tested on phylogenetically closely related species, for example Encephalartos.…”

Section: Discussionmentioning

confidence: 99%

Exposing the illegal trade in cycad species (Cycadophyta:Encephalartos) at two traditional medicine markets in South Africa using DNA barcoding

Williamson

Maurin

Shiba

et al. 2016

Genome

View full text Add to dashboard Cite

Species in the cycad genus Encephalartos are listed in CITES Appendix I and as Threatened or Protected Species in terms of South Africa's National Environmental Management: Biodiversity Act (NEM:BA) of 2004. Despite regulations, illegal plant harvesting for medicinal trade has continued in South Africa and resulted in declines in cycad populations and even complete loss of sub-populations. Encephalartos is traded at traditional medicine markets in South Africa in the form of bark strips and stem sections; thus, determining the species traded presents a major challenge due to a lack of characteristic plant parts. Here, a case study is presented on the use of DNA barcoding to identify cycads sold at the Faraday and Warwick traditional medicine markets in Johannesburg and Durban, respectively. Market samples were sequenced for the core DNA barcodes (rbcLa and matK) as well as two additional regions: nrITS and trnH-psbA. The barcoding database for cycads at the University of Johannesburg was utilized to assign query samples to known species. Three approaches were followed: tree-based, similarity-based, and character-based (BRONX) methods. Market samples identified were Encephalartos ferox (Near Threatened), Encephalartos lebomboensis (Endangered), Encephalartos natalensis (Near Threatened), Encephalartos senticosus (Vulnerable), and Encephalartos villosus (Least Concern). Results from this study are crucial for making appropriate assessments and decisions on how to manage these markets.Key words: cycads, core barcoding regions, muthi, nrITS, trnH-psbA. Résumé

show abstract

Identification of market adulterants in East Indian sandalwood using DNA barcoding

Cited by 30 publications

References 16 publications

An Effective Wood DNA Extraction Protocol for Three Economic Important Timber Species of India

An Effective Wood DNA Extraction Protocol for Three Economic Important Timber Species of India

Assessment of the applicability of wood anatomy and DNA barcoding to detect the timber adulterations in Sri Lanka

Exposing the illegal trade in cycad species (Cycadophyta:Encephalartos) at two traditional medicine markets in South Africa using DNA barcoding

Contact Info

Product

Resources

About