Identification of Manganese Superoxide Dismutase from Sphingobacterium sp. T2 as a Novel Bacterial Enzyme for Lignin Oxidation

Abstract

Copyright and reuse:The Warwick Research Archive Portal (WRAP) makes this work of researchers of the University of Warwick available open access under the following conditions. Copyright © and all moral rights to the version of the paper presented here belong to the individual author(s) and/or other copyright owners. To the extent reasonable and practicable the material made available in WRAP has been checked for eligibility before being made available.Copies of full items can be used for personal research or study, educational, or not-forprofit purposes without prior permission or charge. Provided that the authors, title and full bibliographic details are credited, a hyperlink and/or URL is given for the original metadata page and the content is not changed in any way. Publisher's statement:This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Chemical Biology, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see: http://dx.doi.org/10.1021/acschembio.5b00298The version presented here may differ from the published version or, version of record, if you wish to cite this item you are advised to consult the publisher's version. Please see the 'permanent WRAP url' above for details on accessing the published version and note that access may require a subscription. centre ligated by three His, one Asp and a water/hydroxide in each active site. We propose that the lignin oxidation reactivity of these enzymes is due to the production of hydroxyl radical, a highly reactive oxidant. This is the first demonstration that MnSOD is a microbial lignin-oxidising enzyme.3

Microbial community structure and dynamics in thermophilic composting viewed through metagenomics and metatranscriptomics

“…Besides their important role in cellular antioxidant defense, some of these enzymes act as microbial lignin-oxidizers, as shown by manganese-SOD from Sphingobacterium sp . T245.…”

Investigation of the Chemocatalytic and Biocatalytic Valorization of a Range of Different Lignin Preparations: The Importance of β-O-4 Content

“…T2 that can oxidise polymeric organosolv and Kraft lignin, generating multiple oxidation products. 35 we undertook a study to assemble a small collection of different lignin preparations, to characterise their structures, and to study their valorisation via both chemocatalytic and biocatalytic methods.…”

Delignification and enhanced gas release from soil containing lignocellulose by treatment with bacterial lignin degraders

“…T2 manganese superoxide dismutase (Rashid et al . ), and also comparing with commercially available lignin peroxidase from Phanerochaete chrysosporium (Sigma‐Aldrich). Doses of 0·2–1·0 mg enzyme g −1 lignocellulose were incubated with 1·0 g pine lignocellulose for 1 h, and the residual solid assayed for lignin content using the Klason method (Kirk and Obst ), and the aqueous sample tested for total phenol content using the Folin–Ciocalteau colorimetric assay (Meda et al .…”