Identification of manganese-responsive microRNAs in Arabidopsis by small RNA sequencing

Abstract

Manganese (Mn) is an important micronutrient for growth and development in plants, however, excess Mn is harmful by disrupting photosynthesis system and inducing oxidative damage in leaves. MicroRNAs (miRNAs) play key roles in regulating Mn toxicity tolerance in plants. Here, we identified Mn toxicity-responsive miRNAs in Arabidopsis by using small RNA sequencing. Eighteen differentially expressed miRNAs were identified in Arabidopsis thaliana seedlings in response to Mn toxicity. These differentially expressed miRNAs are involved in regulating nutrition homeostasis, transport, stress response, and developmental processes. Our results indicated that these miRNAs play a key role in Mn toxicity response in plants.

Whispers of Nourishment: Unveiling the Role of Non-Coding RNA in Plant Nutrient Availability

“…Key targets among the Mn-responsive miRNAs were found to be leucine-rich repeat-resistant proteins, receptor kinase proteins, and calcium-dependent protein kinases [83]. Furthermore, recent research focusing on Arabidopsis has demonstrated differential expression of miRNAs linked to cell growth, nutrient balance, and ion transport under Mn stress conditions [84].…”

MicroRNA: A Dynamic Player from Signalling to Abiotic Tolerance in Plants

“…Based on the fact that miRNAs can be transferred between plants as a signal, I believe that miRNA genes capable of regulating abiotic stress in plants can be overexpressed in one plant and transferred to another plant via miRNAs to improve abiotic stress resistance in both plants, promote plant growth and development and ultimately increase food production. Alkenyl-and hydroxyalkyl-producing genes [129] In summary, miRNAs are essential for the regulation of mRNA translation in plants, and research exploring the mechanisms of miRNA downstream target gene action could provide a more theoretical basis for improving food production and security.…”

The Role of MicroRNA in Plant Response to Abiotic Stress

“…In the future researchers focus on miRNAs, where genomic information is scarce, will be of great significance in broadening the scope of species and research areas. Heavy metals-Cd MicroRNA-167 Zea mays [117] Heavy metals-Cd MicroRNA-393 Zea mays [117] Heavy metals-Cu MicroRNA-398 Grape VvCSD1 and VvCSD2 [63] Heavy metals-Al MicroRNA-160 Sugarcance [117] Heavy metals-Al MicroRNA-162 Sugarcance [117] Heavy metals-Al MicroRNA-164 Sugarcance [117] Heavy metals-Al MicroRNA-166 Sugarcance [117] Heavy metals-Al MicroRNA-167 Sugarcance [117] Nutrients-Zn MicroRNA-158 Upregulated Brassica juncea FUT1 [118] Nutrients-K MicroRNA-169 Triticum aestivum Pentose pathway [119] Nutrients-N MicroRNA-169 Downregulated Arabidopsis thaliana HAP2 [81] Nutrients-B MicroRNA-319 Upregulated Riticum aestivum MYB transcription factor [92] Nutrients-K MicroRNA-319 Downregulated Hordeum vulgare TCP [92] Nutrients-K MicroRNA-396 Downregulated Hordeum vulgare GRF [83] Nutrients-P MicroRNA-399 Downregulated Arabidopsis thaliana Ubiquitin conjugase E2 [117] Nutrients-Mn MicroRNA-781 Upregulated Arabidopsis thaliana MCM2 [117] Nutrients-Mn MicroRNA-826 Upregulated Arabidopsis thaliana Alkenyl hydroxalkyl producing 2 [117] Author Contributions: All the authors contributed to the present form of the manuscript. Z.M collected the data, drafted the manuscript; Z.M.…”