Identification of Male Specific Molecular Markers in Date Palm Sewi Cultivar

Ageez, Amr; Madboly, E. A.

doi:10.21608/ejgc.2011.10787

Cited by 6 publications

(6 citation statements)

References 15 publications

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“…In addition RDA02 primer amplified a male specific fragment of size 1000 bp (Figure. 2 a), this in agreement with the results of previous report in date palm plant [35]. RDA21 primer amplified two male specific fragments of 1000 and 1400 bp ( Figure.…”

Section: Resultssupporting

confidence: 92%

“…2 b). In contrast [35] amplified a band with RDA21 of size 900 bp. There is a huge difference in date palm cultivars grown under environmental conditions of Sudan [2].…”

Section: Resultsmentioning

confidence: 99%

“…The current study is aims to examine the validation of some DNA molecular marker (RAPD and ISSR), which have been used previously on other cultivars. RAPD and ISSR primers used in this study have been used previously by [1], [35] and [36] for sex determination in date palm genotypes. The DNA concentration of 15 date palm accessions ranged from 0.59 to 22.25 ng/ μl and purity ranged from 1.01 to 1.87 (Table 2).…”

Section: Resultsmentioning

confidence: 99%

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Validation of RAPD and ISSR Markers Used for Sex Determination in Date Palm Grown under Sudan Conditions

Mohammed

Mohamed

2019

Advanced Research in Life Sciences

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Date palm is one of the most important economical crops in the world. Sex determination of date palm in early stage is a prerequisite for breeding and cultivation. The aim of this study is to validate RAPD and ISSR markers for sex identification of date palm genotypes grown under Sudan conditions. DNA was extracted from ten seedlings and five male and female plants using CTAB method. Eight primers, six RAPD and two ISSR primers were examined for their validation in sex determination of date palm genotypes. PCR amplification was performed using these primers. Four RAPD primers OPA02, OPJ-09, RD A02 and RD A21 were amplified male specific band with size of 1000, 1100, 1000 and 1400 pb respectively, while ISSR markers could not. The specific bands were observed clearly among all male genotypes and absent in female samples unknown samples irrespective of genotypes. Our results could be useful for sex determination of date palm sex in seedling stage and would promote date palm cultivation and production.

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Section: Resultssupporting

confidence: 92%

“…2 b). In contrast [35] amplified a band with RDA21 of size 900 bp. There is a huge difference in date palm cultivars grown under environmental conditions of Sudan [2].…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Validation of RAPD and ISSR Markers Used for Sex Determination in Date Palm Grown under Sudan Conditions

Mohammed

Mohamed

2019

Advanced Research in Life Sciences

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“…It is more practicable to make a selection in the nursery of healthy females, along with a few healthy males, to establish a seedling-date plantation. Current molecular studies (Aberlenc-Bertossi, et al, 2010;Ageez and Madboly, 2011;Bekheet and Hanafy, 2011;Cherif et al, 2012;Elmeer and Mattat, 2012;Moghaieb et al, 2010;Younis et al, 2008) are expected to provide the means to identify the gender of seedlings at an early stage and thereby simplify propagation and breeding. Early selection of young seedlings could enhance date palm breeding programs and generate experimental male and female genetic stocks (Siljak-Yakovlev et al, 1996).…”

Section: Seedling Date Palm Propagationmentioning

confidence: 99%

Seedling date palms (Phoenix dactylifera L.) as genetic resources

Johnsonbr¹,

Jain²

2013

Emir. J. Food Agric

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An accelerating worldwide trend toward planting elite cultivars is leading to genetic erosion and a narrowing of the gene pool upon which the date-palm industry is based. Large numbers of seedling dates are known in many major date-growing countries, as well as in naturalized populations in Spain and the Americas where the palm was intentionally introduced. Seedling dates growing under different climatic conditions from those of the major production areas represent potential genetic resources that should be evaluated for desirable traits. Utilizing modern biotechnology, traits such as disease and pest resistance, hardiness, tolerance of salty soils and improved fruit quality and quantity potentially can be transferred to elite cultivars to sustain and further improve fruit production. Specific examples of important seedling date palm populations in Spain, Peru and Mexico are discussed, as well as new cultivars derived from seedlings in the United States. Research on seedling date populations is recommended, along with the establishment of ex situ germplasm collections of promising specimens as living plants, cold storage of seeds or cryopreservation.

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“…This studies included the uses of isozymes [7], peroxydases [8], molecular marker tool such random amplified polymorphic DNA (RAPD) [9,10], PCR-based restriction fragment length polymorphism (PCR-RFLP) approach [11], microsatellites [12], and recently; PCR-based approaches (RAPD and SCoT) [13].…”

Section: Introductionmentioning

confidence: 99%

Novel Set of Sex-Specific PCR-Based Markers Reveals New Hypothesis of Sex Differentiation in Date Palm

Mohamed¹

2015

JPS

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Abstract:The date palm (Phoenix dactylifera L.), 2n=36, is a dioecious long-lived monocotyledonous plant, with separate male and female trees. The dioecy represents the major challenge in development of breeding programs as it is impossible to distinguish tree gander till they flower approximately five to eight years after planting. Although, during the past two decades numerous attempts to develop molecular markers can discriminate among male and female trees in date palm. But, to date, sex-differentiation mechanism is still uncertain and there is no reliable way to determine the sex of date palm plants before reproductive age across all cultivars. Here, we employed an effective three novel gene-targeting marker approaches (SCoT, CDDP and ITAP) in additions to AFLP, in an attempt to develop a novel set of reliable sex-specific PCR-based markers can helping in early gender determination in Egyptian date palm trees. A set of 26 SCoT, 21 CDDP, 18 ITAP and 14 AFLP primers/primer combinations (PCs) were applied against twelve date palm genotypes belonging to three superior Egyptian date palm cultivars to identify any sex-specific markers. Four SCoT (SCoT1, SCoT24, SCoT26 and SCoT35), two CDDP (CDDP4 and CDDP6), one ITAP (ITAP-8/1) and one AFLP (AFLP-4/1) primer/PC exhibited differential fragments/bands between males and females. These differential bands were gel extracted and cloned for subsequent sequencing analysis. Three of the sequenced bands found to be contain more than one sequence. BLAST analysis results indicated that the eleven sequences generated from different gene-targeting marker systems (SCoT, CDDP and ITAP) revealed main similarity with master transcription factors, transcriptional activators/repressors and regulatory proteins involved in plant hormone signal transduction pathways, plant development and biosynthesis of secondary metabolites, playing important role in different types of abiotic and biotic stresses in date palm or oil palm. We speculate that kind of similarity is not just a coincidence. Our results reveals hypothesis that sex differentiation is a complex but well-organized process that involves endogenous and exogenous factors regulate and control the changes in gene expression, physiology, metabolism and architecture of the plant. These results represent the first case-study focusing on the applications of CDDP, ITAP and SCoT techniques as a novel gene targeting markers in sex-determination in date palm. Moreover, indicate that sex-differentiation process have to be addressed at system biology level for deep and better understanding. This developed sex-specific markers expected to be helpful in distinguish the gander in date palm at earliest stages.

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Identification of Male Specific Molecular Markers in Date Palm Sewi Cultivar

Cited by 6 publications

References 15 publications

Validation of RAPD and ISSR Markers Used for Sex Determination in Date Palm Grown under Sudan Conditions

Validation of RAPD and ISSR Markers Used for Sex Determination in Date Palm Grown under Sudan Conditions

Seedling date palms (Phoenix dactylifera L.) as genetic resources

Novel Set of Sex-Specific PCR-Based Markers Reveals New Hypothesis of Sex Differentiation in Date Palm

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